A kind of adipose stem cell composition for wound repair
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A technology of fat stem cells and wound repair, applied in animal cells, drug combinations, vertebrate cells, etc., can solve the problems of poor effect and poor inhibition of scar production
Active Publication Date: 2020-07-31
优赛生命科学发展有限公司
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Chinese patent CN107320768A discloses an auxiliary material for wound repair containing induced pluripotent stem cell extract, which utilizes the ability of induced pluripotent stem cell extract to induce transformation of fibroblasts, and its main effect is to promote wound repair And antibacterial, but its effect on severe skin wounds and burns is poor, especially in the repair process of severe wounds, the inhibitory effect on scar production is poor
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preparation example Construction
[0014] 1. The preparation of adipose stem cell culture products adopts the following methods:
[0015] A) Add adipose-derived mesenchymal stem cells to 5×10 4 The inoculation amount of / ml is inoculated in the medium, and described medium is the basal medium containing induction culture reagent, and described basal medium is the low-sugar DMEM medium containing 10% volume fraction fetal bovine serum, and described induction culture reagent dexamethasone, diammonium glycyrrhizinate, vitamin D 3 and retinoic acid, the amount of induction culture reagent in the culture medium is: dexamethasone (A 1 ) μmol / L, diammonium glycyrrhizinate (A 2 )μmol / L, vitamin D 3 (A 3 ) μmol / L, retinoic acid (A 4 ) 1~3μmol / L;
[0016] B) Place the culture dish inoculated with adipose-derived mesenchymal stem cells on B 1 % concentration of CO 2 , Cultivate under the condition of 37°C, replace the medium every 3 days; co-cultivate for 7 days;
[0017] C) After the culture is completed, remov...
preparation Embodiment
[0036] The composition is prepared as a liquid dressing, prepared as follows:
[0037] 1) Dissolve the adipose stem cell culture product in water, add the prescribed amount of hydroxypropyl methylcellulose to fully dissolve, and obtain liquid A;
[0038]2) Dissolve the prescribed amount of chitosan in acetic acid solution to obtain chitosan acetic acid solution, slowly add liquid A into the chitosan acetic acid solution, stir evenly, adjust the pH to 6.5~6.8 with 0.1M NaOH solution, and make up the remaining Amount of water, that is.
[0039] The formula of preparation embodiment sees the following table (unit: weight percent %)
[0040] Numbering Adipose stem cell culture product / dosage Chitosan dosage / % HPMC dosage / % 1 Example 1 / 1% 1.5 0.5 2 Example 2 / 2% 2.5 1 3 Example 3 / 1% 2 0.5 4 Example 4 / 2% 1.5 1 5 Example 5 / 2% 2.5 0.5 6 Example 6 / 1% 2 1
[0041] The composition obtained in the preparation example uses...
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Abstract
An adipose stem cell composition for wound repair, characterized in that the composition is composed of an adipose stem cell culture product with an active ingredient of 1-2% by weight and at least one pharmaceutical excipient that can be used externally. Adipose-derived stem cell culture products were prepared as follows: A) inoculated adipose-derived mesenchymal stem cells in the culture medium, B) inoculated the culture dish inoculated with adipose-derived mesenchymal stem cells in 10-15% CO 2， Culture at 37°C C) After the culture is completed, remove the medium and collect the cells, wash the cells, homogenate the cells, and centrifuge the homogenate to get the supernatant, put the supernatant in a dialysis bag, and dialyze with PBS at 4°C for 8~ After 24 hours, the solution in the dialysis bag was freeze-dried to be used as a cultured product of adipose stem cells.
Description
technical field [0001] The present invention relates to a composition for external use. Background technique [0002] Adipose-derived stem cells (ASCs) were isolated from adipose tissue for the first time in 2001, because they have the same high proliferation and self-renewal ability as bone marrow-derived stem cells (BMSCs) , a large number of ASCs can be isolated from adipose tissue, and can differentiate into mesoderm and non-mesoderm cells, making it a hot spot in the application of adult stem cells in regenerative medicine, and is widely used in skin tissue regeneration, cartilage and bone Tissue regeneration, wound healing and many other research fields. [0003] In the study of wound repair, how to suppress or even eliminate the generation of scar is a difficult problem in existing clinical medicine. Chinese patent CN107320768A discloses an auxiliary material for wound repair containing induced pluripotent stem cell extract, which utilizes the ability of induced plu...
Claims
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