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Preparation method of silver doped carbon dot based NO release-type wound dressing, and product and applications of product

A technology of wound dressing and silver doping, which is applied in the fields of medical formula, medical science, bandages, etc., and can solve problems such as high doses

Active Publication Date: 2020-10-23
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Ag + Combined with the dual antibacterial effect of CDs, but still requires a higher dose to achieve the desired antibacterial effect

Method used

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  • Preparation method of silver doped carbon dot based NO release-type wound dressing, and product and applications of product
  • Preparation method of silver doped carbon dot based NO release-type wound dressing, and product and applications of product
  • Preparation method of silver doped carbon dot based NO release-type wound dressing, and product and applications of product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of NO-releasing wound dressing based on silver-doped carbon dots

[0039] Step 1: Weigh 1mg of CA, add deionized water, stir at 500rpm for 15min at 25°C, and set the volume to 100mL for mixing.

[0040] Step 2. Transfer 30 mL of the stirred solution into a polytetrafluoroethylene (Teflon)-lined autoclave and heat at 170° C. for 5 hours.

[0041] In step 3, after the reactor is naturally cooled to room temperature, a carbon dot solution is obtained.

[0042] Step 4, 100 μL of carbon dot solution was added dropwise to 800 μL of 10 mM AgNO with stirring at 500 rpm 3 The solution was reacted for two minutes, and then the mixture solution was reacted at 90° C. for 60 minutes to obtain a purple-red Ag / CDs composite solution.

[0043] Step 5, dialyze the above solution for 6 hours with a dialysis bag with a molecular weight cut-off of 8KD-14KD, and then add 200 μL of L-arginine solution (manufacturer: McLean, model: L800637) to the solution obtained after the dia...

Embodiment 2

[0046] Preparation of NO-releasing wound dressing based on silver-doped carbon dots

[0047] Step 1: Weigh 1mg of CA, add deionized water, stir at 500rpm for 15min at 25°C, and set the volume to 100mL for mixing.

[0048] Step 2. Transfer 30 mL of the stirred solution into a polytetrafluoroethylene (Teflon)-lined autoclave and heat at 170° C. for 5 hours.

[0049] In step 3, after the reactor is naturally cooled to room temperature, a carbon dot solution is obtained.

[0050] Step 4, 100 μL of carbon dot solution was added dropwise to 800 μL of 10 mM AgNO with stirring at 500 rpm 3 The solution was reacted for two minutes, and then the mixture solution was reacted at 90° C. for 30 minutes to obtain a purple-red Ag / CDs composite solution.

[0051] Step 5, dialyze the above solution for 6 hours with a dialysis bag with a molecular weight cut-off of 8KD-14KD, and then add 200 μL of L-arginine solution (manufacturer: McLean, model: L800637) to the solution obtained after the dia...

Embodiment 3

[0053] Preparation of NO-releasing wound dressing based on silver-doped carbon dots

[0054] Step 1: Weigh 1mg of CA, add deionized water, stir at 500rpm for 15min at 25°C, and set the volume to 100mL for mixing.

[0055] Step 2. Transfer 30 mL of the stirred solution into a polytetrafluoroethylene (Teflon)-lined autoclave and heat at 170° C. for 5 hours.

[0056] In step 3, after the reactor is naturally cooled to room temperature, a carbon dot solution is obtained.

[0057] Step 4, under stirring at 500rpm, 100 μL of carbon dot solution was added dropwise to 800 μL concentration of (10 mM AgNO 3 The solution was reacted for two minutes, and then the mixture solution was reacted at 90° C. for 90 minutes to obtain a purple-red Ag / CDs composite solution.

[0058] Step 5, dialyze the above solution for 6 hours with a dialysis bag with a molecular weight cut-off of 8KD-14KD, and then add 200 μL of L-arginine solution (manufacturer: McLean, model: L800637) to the solution obtain...

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Abstract

The invention discloses a preparation method of a silver doped carbon dot based NO release-type wound dressing, and a product and applications of the product. The preparation method includes as follows: placing a chlorhexidine acetate aqueous solution into a reactor, and performing reaction under high temperature; after the reactor is naturally cooled to room temperature, taking out the carbon dotsolution, dropwise adding the obtained solution into an AgNO3 solution, and performing reaction under the high temperature; obtaining a purplish red Ag / CDs composite solution after reaction, and adding L-arginine to stir after dialysis; and performing further dialysis, and performing freeze drying to obtain the silver doped carbon dot based NO release-type wound dressing. Compared with other wound dressings, the final dressing sample can react with H2O2 and functionally release NO; the wound dressing has multiple antibacterial mechanisms, so that antibacterial effects can be greatly enhanced;through bacteriostatic zone experiments, efficient antibacterial activity can be shown; and thus, the wound dressing can be widely applied to the disinfection and antibiosis of wounds, and also can reduce the risks of increased bacterial resistance.

Description

technical field [0001] The invention belongs to the technical field of antibacterial carbon nanomaterials, and in particular relates to a preparation method of an NO-releasing wound dressing based on silver-doped carbon dots and its product and application. Background technique [0002] During wound repair after exposure to any injury, bacterial infection occurs in the tissue, and a severe inflammatory response always results in unsuccessful wound healing. Fighting bacterial infections with effective antibacterial wound dressings can minimize the inflammatory process. With the continuous emergence of drug-resistant bacteria, traditional antibacterial drugs are facing more and more severe challenges in hygiene prevention and disease treatment, and people's demand for high-efficiency antibacterial materials is becoming increasingly urgent. [0003] Carbon dots (CDs) are a general term for a class of low-cost carbon nanomaterials, which were first reported in 2004 and have uni...

Claims

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Application Information

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IPC IPC(8): A61L15/18A61L15/46A61L15/20A61L15/42
CPCA61L15/18A61L15/46A61L15/20A61L15/42A61L2300/104A61L2300/206A61L2300/404A61L2300/45A61L2300/11A61L2400/12A61L2300/114
Inventor 周宁琳沈健张盼孙宝宏楚晓红王玉丽张启成王志轩吴凡
Owner NANJING NORMAL UNIVERSITY
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