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Epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing and preparation method thereof

A technology of p-hydroxyphenylpropionic acid and polylysine, which is applied in the field of medical materials, can solve the problems of limited use, low mechanical strength, poor biocompatibility, etc., and achieve excellent biocompatibility and biodegradability Sexuality, prevention of wound infection, excellent inhibitory effect

Active Publication Date: 2015-08-26
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hydrogels prepared by radiation methods generally have low mechanical strength, require high equipment, and are limited in wide application.
[0008] In summary, antibacterial wound hydrogel dressings have various deficiencies in antibacterial materials and hydrogel preparation methods, such as poor biocompatibility, or poor antibacterial properties, etc.

Method used

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  • Epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing and preparation method thereof
  • Epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing and preparation method thereof
  • Epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Dissolve p-hydroxyphenylpropionic acid in a blend solvent of N,N-dimethylformamide (DMF) and deionized water (RO water). The volume ratio of DMF to RO water is 1:3. The dosage of the acid is 1g / 100mL of the blending solvent, stirring and mixing evenly. Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and activate it under ice-bath conditions for 5h. Add ε-polylysine dissolved in RO water into the activated system, and react for 15 hours at room temperature. The molar ratio of each substance is as follows: EPL (NH 2 ):HPA=1:1, EDC:HPA=1:1, EDC:NHS=1:1. The reacted solution was transferred to a dialysis bag and dialyzed in RO water for 5 days. The obtained purified solution after dialysis was freeze-dried to obtain EPL-HPA copolymer with a yield of 45%.

Embodiment 2

[0052] Dissolve p-hydroxyphenylpropionic acid in a blend solvent of N,N-dimethylformamide (DMF) and deionized water (RO water). The volume ratio of DMF to RO water is 1:3. The dosage of the acid is 1g / 100mL of the blending solvent, stirring and mixing evenly. Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and activate it under ice-bath conditions for 5h. Add polylysine dissolved in RO water into the activated system, and react for 15 hours at room temperature. The molar ratio of each substance is as follows: EPL (NH 2 ):HPA=1:3, EDC:HPA=1:1, EDC:NHS=1:1. The reacted solution was transferred to a dialysis bag and dialyzed in RO water for 5 days. The obtained purified solution after dialysis was freeze-dried to obtain EPL-HPA copolymer with a yield of 87%.

Embodiment 3

[0054] Dissolve p-hydroxyphenylpropionic acid in a blend solvent of N,N-dimethylformamide (DMF) and deionized water (RO water). The volume ratio of DMF to RO water is 1:3. The dosage of the acid is 1g / 100mL of the blending solvent, stirring and mixing evenly. Add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and activate it under ice-bath conditions for 5h. Add polylysine dissolved in RO water into the activated system, and react for 15 hours at room temperature. The molar ratio of each substance is as follows: EPL (NH 2 ):HPA=1:5, EDC:HPA=1:1, EDC:NHS=1:1. The reacted solution was transferred to a dialysis bag and dialyzed in RO water for 5 days. The obtained purified solution after dialysis was freeze-dried to obtain EPL-HPA copolymer with a yield of 63%.

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Abstract

The invention discloses a preparation method of epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing. The method comprises the following steps: dissolving p-hydroxybenzene propanoic acid in a blended solvent of an organic solvent and deionized water, and stirring and uniformly mixing; adding 1-(3-dimethylamino propyl)-3-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide, and performing activation in an ice-bath condition; adding epsilon-polylysine dissolved by deionized water into an activated system, so as to enable the epsilon-polylysine to react with the activated system at the room temperature; transferring an obtained system into a dialysis bag, so as to dialyze the system; freezing and drying dialyze purified solution, so as to obtain epsilon-polylysine-p-hydroxybenzene propanoic acid copolymer; respectively preparing a stock solution A and a stock solution B by taking PBS buffer solution as a solvent, respectively adding the stock solution A and the stock solution B into a tube A and a tube B of a double-end injector, and slowly pushing out the stock solution A and the stock solution B, so as to obtain the uniform and transparent epsilon-polylysine-p-hydroxybenzene propanoic acid antibiotic hydrogel dressing.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to an ε-polylysine-p-hydroxyphenylpropionic acid hydrogel and a preparation method thereof. Background technique [0002] It is inevitable that people cause various injuries to human skin in daily life and work. According to the statistics of the World Health Organization, in 1990, the number of deaths from various traumas in the world was about 5.1 million, and it is expected to increase to 8.4 million in 2020. According to the statistics of the Ministry of Health, in 2005, trauma was the fifth cause of death in both urban and rural areas of our country, accounting for 6.17% of the total death toll. Therefore, trauma is a kind of disease that cannot be ignored. Wound dressings are medical materials that can temporarily protect wounds, prevent pollution, and promote healing. Using wound dressings is one of the effective means of wound treatment. [0003] An ...

Claims

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Application Information

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IPC IPC(8): A61L15/26A61L15/44A61L15/42
Inventor 徐虹王瑞徐得磊迟波冯小海梁金丰
Owner NANJING UNIV OF TECH
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