Preparation method and application of fluorescent platinum nanoclusters for optical imaging of blood system suspension cells
A technology for suspended cells and blood systems, applied in the field of new materials, can solve the problems of difficult detection and difficult calibration of suspended cells, and achieve the effects of small size, easy operation, and high quantum fluorescence yield.
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Embodiment 1
[0017] Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water 2 PtCl 6 and polyvinylimine PEI, final H 2 PtCl 6 and PEI concentrations were 0.1 mmol / L and 2 mmol / L, respectively. After fully mixing at 25 °C for 30 min, 0.1 mmol of L-ascorbic acid was added, the solution was heated to 90 °C, reacted for 2 h and cooled naturally to obtain a light yellow solution. The above crude product was ultracentrifuged and dialyzed with a dialysis bag for 1 day to obtain the final fluorescent platinum nanoclusters, which emitted light blue fluorescence under ultraviolet light irradiation.
[0018] Fluorescent imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters obtained in the above step and add them to the sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final concentration of fluorescent platinum nanoclusters is 0.001mmol / L. Purified air (5% CO) in a sterile environment at 37 °C 2 ) under...
Embodiment 2
[0020] Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water 2 PtCl 6 and polyvinylimine PEI, final H 2 PtCl 6 and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After mixing thoroughly at -4 °C for 24 h, 0.1 mmol of L-ascorbic acid was added, the solution was heated to 90 °C, reacted for 4 days, and cooled naturally to obtain a yellow solution. The above crude product was ultracentrifuged and dialyzed with a dialysis bag for 2 days to obtain the final fluorescent platinum nanoclusters, which emitted yellow fluorescence under ultraviolet light irradiation.
[0021] Fluorescent imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters obtained in the above step and add them to the sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final concentration of fluorescent platinum nanoclusters is 0.001mmol / L. Purified air (5% CO) in a sterile environment at 37 °C 2 ) under t...
Embodiment 3
[0023] Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water 2 PtCl 6 and polyvinylimine PEI, final H 2 PtCl 6 and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After mixing thoroughly at 60 °C for 1.5 h, 0.1 mmol of L-ascorbic acid was added, the solution was heated to 90 °C, reacted for 4 days, and cooled naturally to obtain a yellow solution. The above crude product was ultracentrifuged and dialyzed with a dialysis bag for 2 days to obtain the final fluorescent platinum nanoclusters, which emitted yellow fluorescence under ultraviolet light irradiation.
[0024] Fluorescent imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters obtained in the above step and add them to the sterile RPMI-1640 culture medium (containing 10% serum) containing BV173 cells. The final concentration of fluorescent platinum nanoclusters is 0.005 mmol / L. Purified air (5% CO) in a sterile environment at 37 °C 2 ) unde...
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