Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of fluorescent platinum nanoclusters for optical imaging of blood system suspension cells

A technology for suspended cells and blood systems, applied in the field of new materials, can solve the problems of difficult detection and difficult calibration of suspended cells, and achieve the effects of small size, easy operation, and high quantum fluorescence yield.

Active Publication Date: 2018-11-20
ZHONGYUAN ENGINEERING COLLEGE
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention proposes a preparation method and application of fluorescent platinum nanoclusters for optical imaging of suspended cells in the blood system, which solves the technical problems of difficult calibration and detection of suspended cells in the prior art. The invention discloses in detail a simple operation, Environmentally friendly, preparation method of platinum nanoclusters with excellent fluorescence properties, and expand the application range of fluorescent nanoplatinum clusters as markers in bioimaging, not only can calibrate adherent cells, but also can achieve optical imaging of suspended cells in the blood system, Realize early identification and diagnosis of blood system diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of fluorescent platinum nanoclusters for optical imaging of blood system suspension cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water 2 PtCl 6 and polyvinylimine PEI, final H 2 PtCl 6 and PEI concentrations were 0.1 mmol / L and 2 mmol / L, respectively. After fully mixing at 25 °C for 30 min, 0.1 mmol of L-ascorbic acid was added, the solution was heated to 90 °C, reacted for 2 h and cooled naturally to obtain a light yellow solution. The above crude product was ultracentrifuged and dialyzed with a dialysis bag for 1 day to obtain the final fluorescent platinum nanoclusters, which emitted light blue fluorescence under ultraviolet light irradiation.

[0018] Fluorescent imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters obtained in the above step and add them to the sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final concentration of fluorescent platinum nanoclusters is 0.001mmol / L. Purified air (5% CO) in a sterile environment at 37 °C 2 ) under...

Embodiment 2

[0020] Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water 2 PtCl 6 and polyvinylimine PEI, final H 2 PtCl 6 and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After mixing thoroughly at -4 °C for 24 h, 0.1 mmol of L-ascorbic acid was added, the solution was heated to 90 °C, reacted for 4 days, and cooled naturally to obtain a yellow solution. The above crude product was ultracentrifuged and dialyzed with a dialysis bag for 2 days to obtain the final fluorescent platinum nanoclusters, which emitted yellow fluorescence under ultraviolet light irradiation.

[0021] Fluorescent imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters obtained in the above step and add them to the sterile RPMI-1640 culture medium (containing 10% serum) containing K562 cells. The final concentration of fluorescent platinum nanoclusters is 0.001mmol / L. Purified air (5% CO) in a sterile environment at 37 °C 2 ) under t...

Embodiment 3

[0023] Preparation method of fluorescent platinum nanoclusters: add H in 3.0 mL water 2 PtCl 6 and polyvinylimine PEI, final H 2 PtCl 6 and PEI concentrations were 0.6 mmol / L and 2 mmol / L, respectively. After mixing thoroughly at 60 °C for 1.5 h, 0.1 mmol of L-ascorbic acid was added, the solution was heated to 90 °C, reacted for 4 days, and cooled naturally to obtain a yellow solution. The above crude product was ultracentrifuged and dialyzed with a dialysis bag for 2 days to obtain the final fluorescent platinum nanoclusters, which emitted yellow fluorescence under ultraviolet light irradiation.

[0024] Fluorescent imaging of suspended cells in the blood system: Take the fluorescent platinum nanoclusters obtained in the above step and add them to the sterile RPMI-1640 culture medium (containing 10% serum) containing BV173 cells. The final concentration of fluorescent platinum nanoclusters is 0.005 mmol / L. Purified air (5% CO) in a sterile environment at 37 °C 2 ) unde...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of new materials, and especially relates to a preparation method and an application of fluorescent platinum nanoclusters for optical imaging of blood system suspension cells. The preparation method comprises the following steps: achieving cooperation of a platinum ion precursor aqueous solution and an amine polymer; performing full complexing by an amine group; adding a reducing agent at a high temperature to airtightly stir; preparing a crude product of the fluorescent platinum nanoclusters; and finally obtaining a water-soluble fluorescent platinum nanocluster after ultracentrifugation and dialysis A prepared fluorescent platinum nanocluster with an ultra-small size can be applied to the optical imaging of the blood system suspension cells. The invention provides the preparation method of the platinum nanoclusters which has the characteristics of simple operation, environmental protection, and excellent fluorescence performance. An adherent cell system can be calibrated; and the preparation method extends the platinum nanoclusters as fluorescent markers for the first time into the optical imaging of the blood system suspension cells, and is of great significance for early identification and diagnosis of blood system diseases.

Description

technical field [0001] The invention belongs to the technical field of new materials, in particular to a preparation method and application of fluorescent platinum nano-clusters for optical imaging of blood system suspension cells. Background technique [0002] In recent decades, the incidence of blood system diseases such as aplastic anemia and myeloproliferative diseases has increased year by year, and the harm they have brought has become increasingly serious. For example, for leukemia, there is no particularly effective treatment method except bone marrow transplantation. Therefore, it is of great significance to study the early identification and diagnosis of hematopoietic neoplasms. Noble metal nanoclusters, because the scale is close to the Fermi wavelength of electrons, the quantum confinement effect causes the original continuous electronic energy state to split into discrete electronic energy states, showing a completely different state from bulk materials and nan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/87C09K11/06B82Y30/00B82Y40/00G01N21/64
CPCB82Y30/00B82Y40/00C09K11/06C09K11/87C09K2211/185G01N21/6486
Inventor 黄鑫罗毅岳献阳王少博于保康
Owner ZHONGYUAN ENGINEERING COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com