The invention discloses a stem 
grafting method for melon 
crop stock-scion identification 
signal research, and belongs to the technical field of agricultural planting. The 
grafting method comprises the following steps: cultivating a melon 
seedling A and a melon 
seedling B; beveling the melon 
seedling A to obtain a scion with a 
cut, 
cutting a stem of the melon seedling B from a 
plant to obtain an in-vitro stock with the 
cut, and 
grafting the scion and the stock to obtain a grafted stem segment; performing healing culture in a healing room; sampling a grafted stem segment interface, a non-grafted scion A and a non-grafted stock B, respectively carrying out 
transcriptome sequencing to collect differential genes of the three, and screening the melon stock-scion early identification 
signal gene through union analysis. According to the method disclosed by the invention, the influence of scion leaves and 
rootstock root systems on grafting interface signals is eliminated, only stem segments are reserved, and grafting among tissues is performed, so that the 
gene screening range is favorably reduced, early 
signal identification key genes are screened out, and a 
technical support is provided for research on grafting incompatibility of melon crops.