The invention discloses a stem
grafting method for melon
crop stock-scion identification
signal research, and belongs to the technical field of agricultural planting. The
grafting method comprises the following steps: cultivating a melon
seedling A and a melon
seedling B; beveling the melon
seedling A to obtain a scion with a
cut,
cutting a stem of the melon seedling B from a
plant to obtain an in-vitro stock with the
cut, and
grafting the scion and the stock to obtain a grafted stem segment; performing healing culture in a healing room; sampling a grafted stem segment interface, a non-grafted scion A and a non-grafted stock B, respectively carrying out
transcriptome sequencing to collect differential genes of the three, and screening the melon stock-scion early identification
signal gene through union analysis. According to the method disclosed by the invention, the influence of scion leaves and
rootstock root systems on grafting interface signals is eliminated, only stem segments are reserved, and grafting among tissues is performed, so that the
gene screening range is favorably reduced, early
signal identification key genes are screened out, and a
technical support is provided for research on grafting incompatibility of melon crops.