Platinum nanocluster with uricase-like activity and preparation method of platinum nanocluster

A platinum nano-cluster technology, which is applied in the field of platinum nano-clusters with uricase-like activity and its preparation, can solve the problems of single catalytic performance and complex synthesis steps, and achieve high catalytic activity, simple preparation method, and easy-to-use effects

Inactive Publication Date: 2020-05-29
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research focuses on elucidating and improving the catalytic performance of nano-platinum, especially the synthesis of complex-morph

Method used

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  • Platinum nanocluster with uricase-like activity and preparation method of platinum nanocluster
  • Platinum nanocluster with uricase-like activity and preparation method of platinum nanocluster
  • Platinum nanocluster with uricase-like activity and preparation method of platinum nanocluster

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of protamine-modified platinum nanoclusters

[0022] Step 1: Add 4.25 mL of 0.4 mg / mL protamine aqueous solution to 4.25 mL of 0.6 mM chloroplatinic acid aqueous solution at a temperature of 25°C and magnetic stirring; Step 2: Pre-mix and stir the obtained mixture for 15 min, with a stirring speed of 1500 r / min, to obtain a protamine-chloroplatinic acid mixed precursor solution; the third step: slowly add 1.5 mL of 10 mM sodium borohydride solution freshly prepared in an ice-water bath to the protamine-chloroplatinic acid In the mixed precursor solution of platinum acid, the reaction was continuously stirred for 2 h. Step 4: Purify the product, store it at 4°C for future use, and obtain a clustered platinum nanozyme whose microstructure and size distribution are as follows: figure 1 with figure 2 shown.

Embodiment 2

[0024] Preparation of protamine-modified platinum nanoclusters

[0025] Step 1: Add 4.25 mL of 0.4 mg / mL protamine aqueous solution to 4.25 mL of 0.6 mM chloroplatinic acid aqueous solution at a temperature of 25°C and magnetic stirring; Step 2: The resulting mixture is premixed and stirred for 30 min, with a stirring speed of 1250 r / min, to obtain a protamine-chloroplatinic acid mixed precursor solution; the third step: slowly add 1.5 mL of 10 mM sodium borohydride solution freshly prepared in an ice-water bath to the protamine-chloroplatinic acid In the mixed precursor solution of platinum acid, the reaction was continuously stirred for 4 h. Step 4: Purify the product and store it at 4°C for future use to obtain a clustered platinum nanozyme.

Embodiment 3

[0027] Preparation of protamine-modified platinum nanoclusters

[0028] Step 1: Add 4.25 mL of 1.0 mg / mL protamine aqueous solution to 4.25 mL of 0.8 mM chloroplatinic acid aqueous solution at a temperature of 25°C and magnetic stirring; Step 2: Pre-mix and stir the obtained mixture for 15 min, with a stirring speed of 1500 r / min, to obtain a protamine-chloroplatinic acid mixed precursor solution; the third step: slowly add 1.5 mL of 10 mM sodium borohydride solution freshly prepared in an ice-water bath to the protamine-chloroplatinic acid In the mixed precursor solution of platinum acid, the reaction was continuously stirred for 2 h. Step 4: Purify the product and store it at 4°C for future use to obtain a clustered platinum nanozyme.

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Abstract

The invention discloses a preparation method of a platinum nanocluster with uricase-like activity. Chloroplatinic acid is used as a reactant metal precursor, protamine is used as a modifier to stabilize and induce platinum nanoparticles to form a multistage cluster structure. The prepared platinum nanocluster has uricase-like activity, and the activity of the platinum nanocluster is comparable with that of natural uricase; the preparation method is simple, easy to operate, one-step in synthesis and easy for batch production; reaction conditions are mild, only normal temperature and normal pressure are needed, safety, no pollution and environmental friendliness are achieved, equipment investment is small, cost is low, and reproducibility is good; the used protein is high in safety and goodin thermal stability, and has quite high nutrition and functionality; the obtained product has the characteristics of no toxicity, good biocompatibility, simple purification and storage, good stability, high catalytic activity and the like, and has an important value in treatment of human hyperuricemia related diseases (such as gout).

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a platinum nano-cluster with uricase-like activity and a preparation method thereof. Background technique [0002] In recent years, with the development of nanomaterials, some nanomaterials have gradually been found to have high-efficiency catalytic activity similar to natural proteases, and overcome the shortcomings of natural enzymes such as complex preparation, high price, and easy inactivation, and are defined as "nanozymes". . Nanozymes have high catalytic activity, low cost, easy storage, good stability, and have the characteristics of nanomaterials. They have broad application prospects in the fields of disease diagnosis, food safety, biosensing, and disease treatment. At present, a variety of nanozymes have been developed for the treatment of various diseases, including tumors, inflammation, Alzheimer's disease, Parkinson's disease, etc., but there are relatively few st...

Claims

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Application Information

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IPC IPC(8): B01J31/28B01J23/42A61K33/243A61K47/42A61P19/06
CPCB01J31/28B01J23/42B01J31/003B01J35/0013A61K33/243A61K47/42A61P19/06
Inventor 刘燕秦玉铃郭荣
Owner YANGZHOU UNIV
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