Preparation method of chicken protein-gold nanocluster and application of chicken protein-gold nanocluster in quercetin detection

A gold nanocluster and egg white technology, applied in nanotechnology, nano-optics, nanotechnology, etc., can solve the problems of low sensitivity, time-consuming, high detection cost, etc., and achieve good biocompatibility, fast and simple method, and high detection sensitivity Good results

Pending Publication Date: 2022-06-03
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional detection methods for quercetin have limitations such as high detection cost, low sensitivity, and long time-consuming, so it is urgent to develop a fast and efficient method to accurately detect quercetin in biological samples

Method used

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  • Preparation method of chicken protein-gold nanocluster and application of chicken protein-gold nanocluster in quercetin detection
  • Preparation method of chicken protein-gold nanocluster and application of chicken protein-gold nanocluster in quercetin detection
  • Preparation method of chicken protein-gold nanocluster and application of chicken protein-gold nanocluster in quercetin detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Weigh 5 mg of egg whites, add 100 μL of water to dissolve; then add 100 μL of chloroauric acid, vortex and stir for 2 minutes; in the second step, add 10 μL of sodium hydroxide solution with a concentration of 1M to the above solution, mix and adjust evenly The pH of the solution was 9, and the solution was heated in a water bath at 37°C for 6 hours to obtain chicken egg white-gold nanoclusters.

Embodiment 2

[0034] Example 2: Weigh 5 mg of chicken egg white, add 100 μL of water to dissolve; then add 100 μL of chloroauric acid, vortex and stir for 2 minutes; in the second step, add 15 μL of sodium hydroxide solution with a concentration of 1M to the above solution, mix and adjust evenly The pH of the solution was 11, and the solution was heated in a water bath at 37°C for 6 hours to obtain chicken egg white-gold nanoclusters.

Embodiment 3

[0035] Example 3: Weigh 5 mg of egg whites, add 100 μL of water to dissolve; then add 100 μL of chloroauric acid, vortex and stir for 2 minutes; in the second step, add 20 μL of sodium hydroxide solution with a concentration of 1M to the above solution, mix and adjust evenly The pH of the solution was 13, and the solution was heated in a water bath at 37°C for 6 hours to obtain chicken egg white-gold nanoclusters.

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Abstract

The invention relates to the technical field of synthesis of gold-platinum nano-clusters, in particular to a preparation method of chicken protein-gold nano-clusters and application of the chicken protein-gold nano-clusters in quercetin detection.The preparation method comprises the following specific steps that egg white serves as a template, and an egg white-gold nano-cluster stock solution is prepared under the specific pH condition; under the excitation wavelength of 365 nm, the gold nanocluster shows red fluorescence, and the maximum emission peak is located at the position of 665 nm; as an effective environment-friendly fluorescent probe, the gold nano-cluster can be used for detecting quercetin (Que) in a solution. The fluorescent gold nano-cluster has unique photophysical characteristics, is simple to prepare, low in toxicity and high in stability, the quercetin detection method is rapid and simple, the detection sensitivity is high, and the fluorescent gold nano-cluster is an ideal fluorescent nano-material applied to the fields of biology and medicine.

Description

technical field [0001] The invention relates to the technical field of gold-platinum nano-cluster synthesis, in particular to a preparation method of chicken egg white-gold nano-cluster and its application in the detection of quercetin. Background technique [0002] Chicken egg white is a high viscosity solution containing about 11% protein and glycoprotein, which contains more than forty kinds of proteins, such as ovalbumin (54%), ovotransferrin (12%), ovomucin (1.5%). %), ovomucoid (10%), etc., which have the functions of moistening the lungs and pharynx, clearing away heat and detoxifying, and enhancing immunity. Ovalbumin contains sufficient amino acid residues, such as tyrosine and cysteine, and cysteine ​​and histidine residues can act as protective agents for metal ions; tyrosine residues can act as reducing agents, in Reduction of precious metal ions under alkaline conditions. In addition, egg white is one of the ideal materials for the synthesis of metal nanoclust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/58G01N21/64B82Y20/00B82Y30/00
CPCC09K11/025C09K11/58G01N21/6402G01N21/6428B82Y20/00B82Y30/00
Inventor 付丁伊周梦艳曹蕾丁姝姝王佳茜
Owner NANTONG UNIVERSITY
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