Preparation method of chicken protein-gold nanocluster and application of chicken protein-gold nanocluster in quercetin detection
A gold nanocluster and egg white technology, applied in nanotechnology, nano-optics, nanotechnology, etc., can solve the problems of low sensitivity, time-consuming, high detection cost, etc., and achieve good biocompatibility, fast and simple method, and high detection sensitivity Good results
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Embodiment 1
[0033] Example 1: Weigh 5 mg of egg whites, add 100 μL of water to dissolve; then add 100 μL of chloroauric acid, vortex and stir for 2 minutes; in the second step, add 10 μL of sodium hydroxide solution with a concentration of 1M to the above solution, mix and adjust evenly The pH of the solution was 9, and the solution was heated in a water bath at 37°C for 6 hours to obtain chicken egg white-gold nanoclusters.
Embodiment 2
[0034] Example 2: Weigh 5 mg of chicken egg white, add 100 μL of water to dissolve; then add 100 μL of chloroauric acid, vortex and stir for 2 minutes; in the second step, add 15 μL of sodium hydroxide solution with a concentration of 1M to the above solution, mix and adjust evenly The pH of the solution was 11, and the solution was heated in a water bath at 37°C for 6 hours to obtain chicken egg white-gold nanoclusters.
Embodiment 3
[0035] Example 3: Weigh 5 mg of egg whites, add 100 μL of water to dissolve; then add 100 μL of chloroauric acid, vortex and stir for 2 minutes; in the second step, add 20 μL of sodium hydroxide solution with a concentration of 1M to the above solution, mix and adjust evenly The pH of the solution was 13, and the solution was heated in a water bath at 37°C for 6 hours to obtain chicken egg white-gold nanoclusters.
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