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A kind of preparation method of gold-silver nanocluster protected by glutathione thiotransferase and its application in the detection of oxytetracycline

A technology of glutathione and silver nanoclusters, applied in chemical instruments and methods, measuring devices, material excitation analysis, etc., can solve the problems of accumulation, water and soil pollution, low selectivity, etc., and achieve good detection sensitivity, low toxicity, Quick and easy results

Active Publication Date: 2021-08-27
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Oxytetracycline is a commonly used broad-spectrum antibacterial drug, but it has the disadvantages of accumulating in bone and causing water and soil pollution. However, the traditional detection method is time-consuming and has low selectivity. Therefore, a convenient, fast and efficient detection method is urgently needed. Glycosylsulfurtransferase protects cells from toxic chemicals, but until now there was no direct way to confirm its interaction with tetracyclines, making it crucial to develop new technologies such as fluorescent sensing

Method used

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  • A kind of preparation method of gold-silver nanocluster protected by glutathione thiotransferase and its application in the detection of oxytetracycline
  • A kind of preparation method of gold-silver nanocluster protected by glutathione thiotransferase and its application in the detection of oxytetracycline
  • A kind of preparation method of gold-silver nanocluster protected by glutathione thiotransferase and its application in the detection of oxytetracycline

Examples

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Effect test

Embodiment 1

[0040] Example 1: Optimization of the ratio of gold ions to silver ions; 120 μL of 10 mM chloroauric acid aqueous solution, 500 μL of 10.0 mg / ml glutathione transferase aqueous solution and 320 μL of ultrapure aqueous solution were mixed and stirred. After 10 s, adjust the pH to 11 by adding 40 μL of 1 M NaOH solution. The solution changed from light yellow to colorless within 1 minute, then 30 μL of 10 mM silver nitrate aqueous solution was added, rapidly heated to 70° C., and reacted for 1 hour.

Embodiment 2

[0041]Example 2: Optimization of the ratio of gold ions to silver ions; 120 μL of 10 mM chloroauric acid aqueous solution, 500 μL of 10.0 mg / ml glutathione transferase aqueous solution and 320 μL of ultrapure aqueous solution were mixed and stirred. After 10 s, adjust the pH to 11 by adding 40 μL of 1 M NaOH solution. The solution changed from light yellow to colorless within 1 minute, and then 20 μL of 10 mM silver nitrate aqueous solution was added, rapidly heated to 70° C., and reacted for 1 hour.

Embodiment 3

[0042] Example 3: Optimization of the ratio of gold ions to silver ions; 120 μL of 10 mM chloroauric acid aqueous solution, 500 μL of 10.0 mg / ml glutathione transferase aqueous solution and 320 μL of ultrapure aqueous solution were mixed and stirred. After 10 s, adjust the pH to 11 by adding 40 μL of 1 M NaOH solution. The solution changed from light yellow to colorless within 1 minute, then 15 μL of 10 mM silver nitrate aqueous solution was added, rapidly heated to 70° C., and reacted for 1 hour.

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Abstract

The present invention relates to the technical field of synthesis of gold-silver nanoclusters, in particular to a method for preparing gold-silver nanoclusters protected by glutathione sulfur transferase and its application in the detection of oxytetracycline. The steps are as follows: Glutathione sulfur transferase is used as a template, and glutathione sulfur transferase-gold-silver nanocluster stock solution is prepared by heating under specific temperature and pH conditions, and further purified by dialysis. Under the excitation wavelength of 395nm, the gold-silver nanoclusters emit red light at 653nm; with rhodamine B as the standard reference, the fluorescence quantum yield is 15.9%. The gold-silver nanocluster prepared by the present invention can be used as an effective environment-friendly fluorescent probe to detect oxytetracycline in solution and living cells, and has unique photophysical properties, ultra-small size, low toxicity and excellent Biocompatibility; the detection method of oxytetracycline is fast and simple, and the detection sensitivity is good. It is an ideal fluorescent nanomaterial used in the fields of biology and medicine.

Description

technical field [0001] The invention relates to the technical field of gold-silver nano-cluster synthesis, in particular to a preparation method of a glutathione-sulfur transferase-protected gold-silver nano-cluster and its application in the detection of oxytetracycline. Background technique [0002] Glutathione sulfur transferase is a family of multifunctional enzymes that can bind to the cytoplasm or cell membrane. It exists in all organisms. Soluble glutathione sulfur transferase is widely distributed throughout the body and plays a role in liver, kidney, brain, etc. play an important role. It catalyzes the reduction of glutathione to generate a variety of electrophilic substances, thereby protecting cells from toxic chemicals such as drugs, endogenous peroxide radicals and toxic metabolites, many endogenous compounds such as prostaglandins And the metabolism of steroids is also related to the glutathione conjugation reaction. Therefore, the main biological function of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06C09K2211/188G01N21/643G01N2021/6417
Inventor 付丁伊李焕钱屹丁姝姝曹蕾
Owner NANTONG UNIVERSITY
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