Preparation method of glutathione S-transferase-gold platinum nano-cluster and application of glutathione S-transferase-gold platinum nano-cluster in aureomycin detection

A glutathione and sulfur transfer technology, applied in nanotechnology, metal processing equipment, chemical instruments and methods, etc., can solve the problems of bacterial drug resistance, poor selectivity, low sensitivity, etc., and achieve unique photophysical characteristics, Excellent biocompatibility and good detection sensitivity

Active Publication Date: 2022-06-03
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chlortetracycline (CTC) is a broad-spectrum tetracycline antibiotic. It is widely used in animal husbandry because it can effectively inhibit the infection of Gram-positive bacteria and negative bacteria and is cheap. It can also be used as a growth-promoting agent for animals. In the feed; but due to the improper dosage, many side effects have also been produced, such as causing water and soil pollution, bacterial resistance, etc.
The traditional detection method for aureomycin has limitations such as high cost, low sensitivity, poor selectivity, and long time-consuming, so it is urgent to develop a fast, efficient and low-cost detection method

Method used

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  • Preparation method of glutathione S-transferase-gold platinum nano-cluster and application of glutathione S-transferase-gold platinum nano-cluster in aureomycin detection
  • Preparation method of glutathione S-transferase-gold platinum nano-cluster and application of glutathione S-transferase-gold platinum nano-cluster in aureomycin detection
  • Preparation method of glutathione S-transferase-gold platinum nano-cluster and application of glutathione S-transferase-gold platinum nano-cluster in aureomycin detection

Examples

Experimental program
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Effect test

Embodiment 1

[0036]Example 1: Add 50μL of 10mM chloroauric acid solution to 125μL of 20mg ml -1 Add 6.25 μL of chloroplatinic acid with a concentration of 10 mM to the above solution, and mix evenly; adjust the pH of the mixed solution to 3, and the mixed solution is heated at 80 °C. The bath was heated for 5 hours, and dialyzed in phosphate buffer for 24 hours with a dialysis bag with a molecular cut-off of 12-14 kDa to obtain glutathione sulfur transferase-gold platinum nanoclusters.

Embodiment 2

[0037] Example 2: Add 50μL of 10mM chloroauric acid solution to 125μL of 20mg ml -1 Add 6.25 μL of chloroplatinic acid with a concentration of 10 mM to the above solution and mix evenly; adjust the pH of the mixed solution to 7, and the mixed solution is heated at 80 °C. The bath was heated for 5 hours, and dialyzed in phosphate buffer with a dialysis bag with a molecular cut-off of 12-14 kDa for 24 hours to obtain the product glutathione thiotransferase-gold platinum nanoclusters.

Embodiment 3

[0038] Example 3: Add 50 μL of 10 mM chloroauric acid solution to 125 μL of 20 mg ml -1 Add 6.25 μL of 10 mM chloroplatinic acid into the above solution, and mix well; adjust the pH of the mixed solution to 11, and the mixed solution is heated at 80 °C. The bath was heated for 5 hours, and dialyzed in phosphate buffer with a dialysis bag with a molecular cut-off of 12-14 kDa for 24 hours to obtain the product glutathione thiotransferase-gold platinum nanoclusters.

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Abstract

The invention relates to the technical field of synthesis of gold-platinum nano-clusters, in particular to a preparation method of glutathione S-transferase-gold-platinum nano-clusters and application of the glutathione S-transferase-gold-platinum nano-clusters in aureomycin detection.The preparation method comprises the following specific steps that glutathione S-transferase serves as a template, a glutathione S-transferase-gold-platinum nano-cluster stock solution is prepared under the specific pH condition, and the glutathione S-transferase-gold-platinum nano-clusters are obtained; and further performing dialysis purification. Under the excitation wavelength of 375 nm, the gold-platinum nano-cluster shows red fluorescence, and two emission peaks are respectively formed at the positions of 470 nm and 656 nm; as an effective environment-friendly ratiometric fluorescent probe, the gold-platinum nano-cluster can be used for detecting chlortetracycline (CTC) in a solution. The gold-platinum nano-cluster disclosed by the invention has unique photophysical characteristics, is almost non-toxic, has excellent biocompatibility, is rapid and simple in aureomycin detection and good in detection sensitivity, and is an ideal fluorescent nano-material applied to the fields of biology and medicine.

Description

technical field [0001] The invention relates to the technical field of gold-platinum nano-cluster synthesis, in particular to a preparation method of a glutathione sulfur transferase-gold-platinum nano-cluster and its application in the detection of chlortetracycline. Background technique [0002] Glutathione S-transferase is a multifunctional enzyme that is widely distributed throughout the body and can bind to the cytoplasm or membrane, and plays an important role in living organisms. Glutathione S-transferase can catalyze a large number of chemical reactions such as nucleophilic aromatic substitution, Michael addition, etc. In vivo, it can catalyze the reduction of endogenous glutathione and exogenous compounds to generate a variety of electrophilic Substances that protect cells from chemicals such as drugs, endogenous peroxide free radicals, and toxic metabolites. Compared with normal tissues, glutathione thiotransferase is highly expressed in some cancer cells and play...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/054B22F1/10C09K11/87G01N21/64B82Y40/00
CPCB22F9/24C09K11/87G01N21/6402G01N21/6428B82Y40/00Y02A50/30
Inventor 付丁伊周梦艳曹蕾丁姝姝王佳茜
Owner NANTONG UNIVERSITY
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