Preparation method of casein-protected gold and silver nanocluster and application of casein-protected gold and silver nanocluster in aureomycin detection

Abstract

The invention relates to the technical field of synthesis of gold and silver nanoclusters, in particular to a preparation method of a casein-protected gold and silver nanocluster and application of the casein-protected gold and silver nanocluster in aureomycin detection. The preparation method comprises the following steps: step 1, weighing casein, adding 750 mu L of water for dissolving, then adding 50 mu L of a sodium hydroxide solution with the concentration of 3M to adjust the pH of the solution, and heating in a water bath at the temperature of 37-70 DEG C for 20 minutes to obtain a solution A; step 2, adding chloroauric acid into the solution A obtained in the step 1, carrying out uniform mixing, then adding a silver nitrate solution, carrying out uniform mixing, and heating in the water bath at the temperature of 37-70 DEG C for 0-4 hours to obtain a solution B; and storing the solution B obtained in the step 2 at the temperature of 4 DEG C to obtain the casein-protected gold and silver nanocluster. The fluorescent gold and silver nanocluster prepared by the preparation method has unique photophysical characteristics, the preparation method is simple, the stability is high, the aureomycin detection method is rapid and convenient, the detection sensitivity is high, the detection limit is low, and the casein-protected gold and silver nanocluster is an ideal fluorescent nano material applied to the fields of biology and medicine.

Description

technical field [0001] The invention relates to the technical field of gold-silver nano-cluster synthesis, in particular to a preparation method of casein-protected gold-silver nano-clusters and its application in the detection of chlortetracycline. Background technique [0002] Casein, which accounts for about 80% of milk proteins, is an amphiphilic copolymer containing phosphorus and having both hydrophilic and hydrophobic amino acid residues. At present, it is mainly used as food raw material or microbial culture medium, and it is also one of the sources of amino acids and calcium and phosphorus for young children. Casein has various physiological functions such as preventing mineral loss, preventing osteoporosis and rickets, treating iron-deficiency anemia, magnesium-deficiency neuritis, etc. absorb. Casein contains a variety of amino acids, especially abundant proline residues. Based on the reported use of proline to synthesize metal nanoclusters, we believe that case...

AI Technical Summary

Problems solved by technology

[0004] Chlortetracycline is a common broad-spectrum tetracycline antibiotic, which can inhibit Gram-positive and Gram-negative bacteria. It can treat typhoid fever, pullorum and other diseases of livestock and poultry. It is also used as a growth-promoting agent in animal feed However, if the dosage is improper or used frequently for a long time, the residues in the animal body will cause serious side effects, such as soil and water pollution, bacterial resistance, and threaten human health

However, the traditional detection method for aureomycin has limitations such as poor stability, low sensitivity, and long time consumption. Therefore, it is urgent to develop a fast and efficient detection method.

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