37results about How to "Achieving Combined Therapy" patented technology

The invention discloses a nanometer medicine particle. The nanometer medicine particle comprises a hydrophobic polymer, a single-layer lipid molecule, an amphipathy macromolecular compound, at least one chemotherapy drug attached to the hydrophobic polymer, and at least one near-infrared photothermal conversion agent, wherein the hydrophobic polymer, the at least one chemotherapy drug attached to the hydrophobic polymer, and the at least one near-infrared photothermal conversion agent form a hydrophobic core; the single-layer lipid molecule surrounds the surface of the hydrophobic core to form an intermediate layer; and the amphipathy macromolecular compound intersperses in the intermediate layer to form a shell. The invention also provides a preparation method of the nanometer medicine particle. The nanometer medicine particle can realize the combined treatment of thermotherapy and chemotherapy and has high biocompatibility; and the preparation method is simple, convenient and easy to carry out.

The invention provides a preparation method of metal coordination supermolecular nanogel. The metal coordination supermolecular nanogel is composed of natural polysaccharide, histidine and metalloporphyrin. The preparation method of the metal coordination supermolecular nanogel comprises the following steps: (1) grafting the histidine to the natural polysaccharide by use of an EDC / DMAP condensation reaction, thereby preparing the histidine-modified natural polysaccharide; and (2) preparing the metal coordination supermolecular nanogel under the metal coordination action of the metalloporphyrin and the histidine. The preparation method is simple and convenient in steps. The nanogel prepared by the method has tumor intracellular pH sensitivity, and excellent biocompatibility and biodegradability, can be taken as a carrier material for drug delivery and controlled release, and meanwhile, is capable of realizing tumor treatment by virtue of combined chemical treatment and photodynamic therapy.

The invention discloses a preparation method of a gold nano-composite targeting drug delivery system. The preparation method comprises the following steps: sequentially adding a chloroauric acid solution and a silver nitrate solution into a hexadecyl ammonium bromide solution, mixing uniformly, then sequentially adding an ascorbic acid solution and a gold nano-particle seed solution, and centrifuging to obtain a gold nano-bar; adding ethyl orthosilicate to obtain a mesoporous silicon oxide nano-material; adding 3-aminopropyl triethylsilane to obtain aminated GNRs@mSiO2; adding a thionyl chloride solution of melphalan to obtain aminated GNRs@mSiO2-MEL; and adding HA-RGD to obtain GNRs@mSiO2-HA-RGD-MEL. According to the preparation method, gold nano-particles with an effect of photothermal therapy are effectively combined with an anti-cancer drug, namely melphalan, thereby realizing combined therapy of tumor thermotherapy and chemotherapy and increasing the therapeutic effect; and by utilizing mesoporous silicon oxide ducts for efficiently loading drugs, the drug loading capacity can be increased.

The invention relates to a preparation method of a hollow silicon-star gold core / shell nano material for wrapping adriamycin. The preparation method comprises the following steps of dissolving an HMSs (Hollow Mesoporous Silica Sphere), ultrasonically dispersing the dissolved HMSs, adding MPTMS (Mercaptopropyltrimethoxysilane) into the dispersed HMSs, introducing nitrogen into an obtained first mixture, refluxing the obtained first mixture in an oil bath, so as to obtain HMSs-SH (Hollow Mesoporous Silica Sphere-Sulfhydryl), afterwards, dissolving the HMSs-SH in water, adding an Au NPs (Aurum Nanoparticles) solution into an obtained second mixture, agitating the obtained second mixture, so as to obtain an HMSs / Au seed, dissolving the HMSs / Au seed in water, ultrasonically dispersing an obtained third mixture, adding a chloroauric acid solution into the dispersed third mixture, agitating an obtained fourth mixture, adding a silver nitrate solution and an ascorbic acid solution into the agitated fourth mixture, continuously agitating an obtained fifth mixture, so as to obtain an HMSs / Au NSs (Normal Saline Solution), dispersing the HMSs / Au NSs in water, adding an HS-PEG-RGD (Hassium-Polyethylene Glycol-Arginine-Glycine-Aspartic Acid) aqueous solution into an obtained sixth mixture, agitating an obtained seventh mixture, so as to obtain an HMSs / Au-PEG-RGD NSs, afterwards, dispersing the HMSs / Au-PEG-RGD NSs into ultrapure water, adding a DOX*HCL aqueous solution into an obtained eighth solution, agitating an obtained ninth mixture in a light-shielding manner, so that the hollow silicon-star gold core / shell nano material for wrapping the adriamycin is obtained. The nano material provided by the invention owns a specific targeting effect and excellent biocompatibility on a U87MG cell, has a synergistic enhancement effect on chemical therapy and photothermal therapy, and is wide in application prospect.

The invention provides a thermosensitive polymer-modified bivalent platinum nanocluster and a preparation method and application thereof. The bivalent platinum nanocluster has a core-shell structure,the inner core comprises zero-valent platinum with divalent platinum on the surface and the shell is a temperature-sensitive polymer. The preparation method utilizes a thermosensitive polymer ligand as a template to synthesize bivalent platinum nanoclusters with different relative contents of divalent platinum and zero-valent platinum. Through adjustment and control of the relative content of divalent platinum and zero-valent platinum, the relative interaction intensity of the photothermal effect and the chemotherapy effect is precisely adjusted and controlled so that photothermal chemotherapy-combined anti-tumor effects are optimized.

The invention relates to a bismuth-doped metal sulfide nanoflower and a preparation method thereof. The preparation method comprises the steps that a solution system containing both intermediate particles and ammonium thiomolybdic tetrahydrate is subjected to a solvothermal reaction to obtain the bismuth-doped metal sulfide nanoflower; the intermediate particles are generated by mixing an A solution and a B solution, wherein the A solution is mainly composed of BiCl3, MnCl2 4H2O and deionized water, and the B solution is mainly composed of K4[Fe(CN)6] 3H2O, citric acid and deionized water; thefinally prepared bismuth-doped metal sulfide nanoflower is of a nanoflower structure formed by winding and twisting nanosheets, wherein the nanosheets are doping type metal sulfide nanosheets obtained through mutual substitution and mosaicism of elemental bismuth, bismuth sulfide, manganese sulfide, ferrous sulfide and molybdenum sulfide on a two-dimensional structure. The preparation method is simple, the prepared nanoflower can achieve the effect that a single structure has multiple functions, and the photothermal conversion efficiency is higher.

The invention provides a nano medicine delivery system for photo-thermal chemotherapy combined therapy and a preparation method. The nano medicine delivery system is medicine carrying micelle, whereinthe medicine carrying micelle is prepared by enabling poly-propylene sulfide-b-poly(N-isopropyl acrylamide-co-N,N-dimethacrylamide) of formula (I) as shown in the specification to polymerize by self,and by coating doxorubicin (DOX) and indocyanine green (IGG) in micelle. The nano medicine delivery system is characterized in that under radiation of near-infrared light of 808nm, because of photo-thermal effects of the indocyanine green, a temperature can be increased, a hydrophilic layer on the surface of the micelle can be turned into a hydrophobic layer, and the uptake of tumor cells upon the medicine carrying micelle can be improved; meanwhile, the medicine carrying micelle can be oxidized by singlet oxygen generated from the indocyanine green in lighting, and thus medicine release canbe promoted. The nano medicine delivery system has the characteristics of dual responsiveness, combined therapy under near-infrared lighting, and good anti-tumor activity.

The invention discloses a preparation method of a calcium phosphate-rapamycin composite drug, a making method of a drug coating balloon and the drug coating balloon. Through compounding of calcium phosphate and rapamycin, the stability of a rapamycin structure can be improved, continous release of the rapamycin structure is facilitated in vivo, the adhesion capacity of rapamycin on the surface of the balloon and the infiltration capacity of rapamycin in tissue are improved, the retention time of the drug after administration is prolonged, the inhibition effect of rapamycin on cells is improved, so that the treatment effect of rapamycin on vascular restenosis diseases is enhanced, the solubility and stability of the drug can be improved by the nano-material coating, the use dosage of the drug is further reduced, and toxic and side effects are relieved or avoided; and due to the slow release effect of the nanoparticles, the peak-valley phenomenon of the blood concentration can be eliminated, a certain treatment concentration is maintained, and adverse reactions caused by instantaneous overhigh blood concentration are effectively prevented; and the nanoparticles can prolong the retention time of the drug in the circulation system and improve the treatment level of the drug.

The invention discloses a nano therapeutic agent and a preparation method and an application thereof. The nano therapeutic agent includes glucose oxidase and a hydrophobic chemotherapy drug located ina hydrophobic cavity of the glucose oxidase. The nano therapeutic agent is a compound of glucose oxidase and the hydrophobic chemotherapy drugs, wherein the glucose oxidase and the hydrophobic chemotherapy drugs are combined by hydrophobic effect; and the nano therapeutic agent can realize targeted delivery of tumor, chemotherapy and hunger-like combined therapy at the same time.

The invention discloses a preparation method of near-infrared light-enriched cysteine-modified bismuth sulfide hollow spheres and applications of the spheres to photothermal treatment and drug releasecontrol, and solves the problem that materials in the prior art have low photothermal conversion efficiency and are difficult to achieve synergistic treatment of light-heat and medicines. The methodfirstly uses polyvinylpyrrolidone as a coordination agent, and prepares a bismuth complex precursor microsphere in a glycerin / ethanol mixed solvent; and then, uses the bismuth complex microsphere as atemplate, selects L-cysteine as a sulfur source and a surface modifier, and performs hydrothermal synthesis to obtain L-Cys / Bi2S3 hollow spheres. The hollow spheres are composed of nanorods with an average particle size of 250-300 nanometers. The bismuth sulfide hollow spheres obtained by the invention have the effect of obviously enhancing near-infrared light capturing ability and photothermal conversion effect, have strong drug load and release control property, and can be used for combined treatment of tumor photothermal ablation and drug chemotherapy.

The invention discloses a photo-thermal rapid controlled-release nano-drug and a preparation method thereof. The nano-drug comprises a carrier, and an active drug and a near-infrared photo-thermal conversion material which are loaded on the carrier; the carrier is a polymer formed by connecting polycaprolactone containing furyl at one end with maleimide polyethylene glycol through a covalent bond; the preparation method comprises the following steps: preparing a carrier by taking furanylation modified polycaprolactone and maleimide polyethylene glycol as raw materials, and coating the carrier with a material with photothermal conversion capability and an active drug. The nano-drug prepared by the invention can rapidly realize rapid controlled release of the drug under near-infrared irradiation to improve the utilization efficiency of the drug, and also can realize combined treatment of chemotherapy and photothermal therapy, thereby greatly improving the treatment effect of tumors.

The invention relates to a preparation method of a drug self-delivery nano preparation activated by near-infrared light and application of the drug self-delivery nano preparation in tumor photoimmunotherapy, and belongs to the field of pharmaceutical preparations. The near-infrared light activated drug self-delivery nano preparation provided by the invention is based on two small molecule drugs approved by FDA and a dopamine shell layer, self-assembly of the novel nano preparation is realized without assistance of a delivery carrier, and a tumor vaccine capable of being intravenously injected is obtained. The photosensitizer provided by the invention can improve the defects of difficult administration and large toxicity of the existing clinical photosensitizer, improves the accumulation and deep penetration of the drug at the tumor site, and can effectively realize tumor photoimmunotherapy under the irradiation of near-infrared light. The method has high application value in treatment of malignant tumors, bacterial infection and superficial skin diseases. The preparation method of the nano preparation provided by the invention is rapid and simple, all components in the nano preparation are approved by FDA, the safety is good, large-scale standardized production is realized, and huge potential is provided for industrial production and clinical transformation of the technology.

The invention discloses an oxidation response type nano-micelle, and a preparation method and application thereof. The oxidation response type nano-micelle is an amphiphilic active oxygen response type nano-drug delivery system, which is constructed by bonding a hydrophobic anti-tumour drug adriamycin and polyethylene glycol monomethyl ether through an oxidation response thioketal bond; an active oxygen response group in the nano-micelle is one of compounds sensitive to hydroxyl radicals, singlet oxygen or active oxygen; and the active oxygen sensitive compound is a thioketal group. The nano-micelle in the invention can generate a large amount of active oxygen and stimulate thioketal bond breakage under the sonodynamic effect, so that the nano-micelle releases the anti-tumour drug adriamycin; the adriamycin directly acts on tumour tissues; furthermore, damage to surrounding healthy tissues is greatly reduced; combined treatment of sonodynamic and chemotherapy is achieved; effective enrichment and time-space controlled release of the medicine at a tumour part are realized; and an effective anti-tumour effect is achieved.

The invention discloses a preparation method of an iron-based metal organic framework composite material with an MOF-On-MOF framework, an obtained product and application, the iron-based metal organic framework composite material is synthesized by adopting an MOF-On-MOF strategy, then drugs such as doxorubicin hydrochloride are loaded in double-layer MOF-On-MOF pore cavities, and the drug-loaded iron-based metal organic framework composite material is obtained. The synthesized composite material is uniform in size, the second MOF layer not only improves the drug loading capacity, but also can be used as a gating entity, and premature drug leakage is avoided. The composite material has good pH and GSH response capability, has peroxidase-like catalytic activity under an acidic condition, can catalyze H2O2 to generate. OH, shows the potential of chemodynamic therapy for treating cancers, well expands the demand of combined treatment of cancers by using chemotherapy and chemodynamic therapy in the practical application field, and has a wide application prospect. And possibility is provided for clinical application.

The invention relates to a preparation method of a boron-containing nano targeted drug, which comprises the following steps: (1) dissolving an amphiphilic polymer in an organic solvent to obtain an organic solution; adding a boron-containing drug into an aqueous solution containing an emulsifier, uniformly mixing, dropwise adding the obtained system into the above organic solution, and stirring toobtain a w / o emulsion; (2) dropwise adding the w / o emulsion into another aqueous solution containing the emulsifier, stirring, and then removing the organic solvent to obtain a w / o / w double emulsion;and freeze-drying the w / o / w double emulsion to obtain the boron-containing nano targeted drug. The preparation process of the boron-containing nano targeted drug is simple and convenient, the synthesis condition is mild, and expanded production is facilitated. Compared with free BPA, the prepared nano targeted drug has the advantages that the hematoma ratio is remarkably increased and reaches upto 4.1: 1, the tumor treatment effect can be remarkably improved, and the nano targeted drug has very good application prospects when being independently used as an anti-tumor drug and being combinedwith paclitaxel and other anti-tumor drugs for use.

The invention provides a calcium-containing micro-precipitation liposome entrapped with a photothermal agent and a chemotherapeutic drug as well as a preparation method and application of the calcium-containing micro-precipitation liposome, and belongs to the technical field of liposomes. The calcium-containing micro-precipitation liposome entrapped with the photothermal agent and the chemotherapeutic drug comprises a calcium-containing micro-precipitation inner core and a phospholipid bilayer shell entrapped outside the calcium-containing micro-precipitation inner core, the calcium-containing micro-precipitation core comprises the photothermal agent, the chemotherapeutic drug, bovine serum albumin and a calcium-containing precipitate; the phospholipid bilayer shell comprises an inner-layer phospholipid material, an outer-layer phospholipid material and cholesterol. According to the calcium-containing micro-precipitation liposome entrapped with the photothermal agent and the chemotherapeutic drug, the half-life period of the photothermal agent is prolonged, the stability is improved, and the treatment effect of the photothermal agent on tumor cells is improved; the toxic and side effects of chemotherapeutic drugs can be obviously reduced, and the therapeutic effect on the tumor cells is improved; the photothermal-chemical combined treatment can be realized, the side effect is small, and the positioning and treatment effects on the tumor cells are excellent.

The invention discloses an antibody, polypeptide and nucleic acid combination treatment targeting carrier as well as its preparation method and application. It mainly solves the insufficient technical problems in the in vivo delivery technology of existing biomacromolecular drugs. The targeting carrier of the present invention is a biomacromolecular drug delivery nanoparticle with a targeting group that is negatively charged or neutral on the surface; The core of the targeting carrier is composed of polypeptides and nucleic acids, and the targeting carrier for combination therapy of antibodies, polypeptides and nucleic acids has a shell, and the shell is composed of irregular cationic lipid membranes and polyanions that are tightly covered and packaged successively. The polyanion wraps on the surface of the nanoparticle through electrostatic and hydrogen bonding.

The present invention provides a multifunctional nano drug delivery carrier targeting lung cancer genes and a preparation and an application thereof. The nano drug delivery carrier comprises a nano gold triangle piece, a surface of the nano gold triangle piece is sequentially connected with a complex poly(sodium 4-styrene sulfonate) and a complex poly(diallyldimethylammonium chloride) to form a nano gold triangle piece complex; and a siRNA nano drug targeting the lung cancer genes adsorbs and is linked to the nano gold triangle piece complex. The siRNA nano drug is connected with GNPs / PSS / PDADMAC, thereby obtaining the multifunctional nano drug delivery carrier having both targeting and photothermal therapy and immunotherapy.

The invention provides a graphene agilawood moxibustion patch and a preparation method thereof. The graphene agilawood moxibustion patch comprises a heating layer, a moxibustion therapy layer and a bottom layer, wherein a pasting layer is arranged at one side of the bottom layer; the moxibustion therapy layer and the heating layer are sequentially arranged at the opposite side, to the side provided with the pasting layer, of the bottom layer from inside to outside; a graphene essential oil mixture is coated at the bottom of the moxibustion therapy layer and is prepared from graphene powder andessential oil through mixing; the essential oil is prepared from the raw materials of 3 to 8 weight parts of agilawood, 1 to 5 weight parts of radix puerariae, 1 to 5 weight parts of eucommia barks,1 to 4 weight parts of liquorice roots, 1 to 5 weight parts of radix notoginseng, 1 to 5 weight parts of poria cocos, 1 to 4 weight parts of radix bupleuri flowers and 1 to 4 weight parts of radix astragali; a graphene nanometer compound electrode is arranged on the surface of the moxibustion therapy layer; a control circuit and a power supply are arranged in the heating layer. The technical problems that the stomach disease treatment effect of the graphene agilawood moxibustion patch in the prior art is poor, the control is not easy and the like are solved. The graphene agilawood moxibustionpatch provided by the invention can be used for treating diseases such as gastritis and indigestion, also has the effects of protecting the liver, and has the obvious treatment effects.

The invention discloses a method for preparing a calcium phosphate-rapamycin compound drug, a method for making a drug-coated balloon and a drug-coated balloon. The stability of the structure of rapamycin is conducive to its sustained release in the body, improving the adhesion ability of rapamycin on the balloon surface, the penetration ability of rapamycin in tissues and prolonging the residence time of the drug after administration, and improving its effect on cells. Inhibitory effect, thereby enhancing the therapeutic effect of rapamycin on vascular restenosis, the nanomaterial coating can increase the solubility and stability of the drug, thereby reducing the dosage of the drug, reducing or avoiding toxic and side effects; the slowing down of nanoparticles The release effect can eliminate the "peak-valley" phenomenon of blood drug concentration, maintain a certain therapeutic concentration, and effectively prevent adverse reactions caused by instantaneously high blood drug concentration; nanoparticles can prolong the residence time of drugs in the circulatory system and improve drug retention. level of treatment.

The invention discloses a guanidinylated nano-drug based on immune activation as well as preparation and application thereof, and belongs to the technical field of medicines. The nano-drug is a micelle type nano-particle formed by self-assembly of a guanidinylation amphiphilic polymer and a hydrophobic chemotherapeutic drug in water; a hydrophilic segment of the guanidinated amphiphilic polymer is polyethylene glycol, and a hydrophobic segment of the guanidinated amphiphilic polymer is a polymer formed by connecting a pH-responsive hydrazone bond with aminoguanidine. The nano material provided by the invention can release hydrophobic chemotherapeutic drugs in an acidic tumor environment, and tumor cells generate a large number of antigens under the action of the chemotherapeutic drugs. Subsequently, the guanidinylated nanomaterial effectively promotes uptake and cross presentation of antigens by antigen presenting cells such as dendritic cells, and further causes cellular immune response. The nano material realizes combined treatment of chemotherapy and immunotherapy, and greatly enhances the tumor treatment effect.

The invention discloses a polypeptide carrier for nucleic acid transfection and use thereof. The polypeptide carrier comprises a shell and an inner core composed of polypeptide and nucleic acid. A surface of the polypeptide carrier is provided with negatively charged or electrically neutral biomacromolecular drug delivery nanoparticles with targeting groups; the shell is formed by sequentially and tightly covering and wrapping an irregular cationic lipid membrane and polyanions; the cationic lipid membrane is provided with a cationic double-layer phospholipid membrane, the polyanions are wrapped on surfaces of the nanoparticles through electrostatic and hydrogen-bond interaction, and the cationic lipid membrane is a cationic liposome or a cationic micelle formed by cationic lipid and neutral lipid or cholesterol or amphiphilic molecules; the polyanions are selected from hyaluronic acid or hyaluronic acid derivatives; and the exposed cationic lipid membrane breaks through an endosome membrane through a membrane melting effect, and polypeptide, nucleic acid and a compound of the polypeptide and the nucleic acid are released into cytoplasm, such that combined treatment of an antibody, the polypeptide and the nucleic acid for cancer with high expression of targeted HER2 is realized.