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Method for synthesizing rare earth metal compound nano cluster and application thereof

A rare earth metal, synthesis method technology, applied in pharmaceutical formulations, echo/ultrasonic imaging agents, preparations for in vivo tests, etc., can solve the problems of difficult deep tissue imaging, weak signal-to-noise ratio, etc., and achieve good targeted imaging Effect, strong targeting, broad medical application prospects

Active Publication Date: 2015-01-28
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nanoparticles have been used as luminescent probes for optical imaging, but there are many disadvantages: first, autofluorescence from organic components of tissues can lead to weak signal-to-noise ratio; second, it is difficult to achieve deep tissue imaging because of the weakened signal of inner tissues The emission wavelength of the probe should be adjusted to the condition of tissue transparency

Method used

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  • Method for synthesizing rare earth metal compound nano cluster and application thereof
  • Method for synthesizing rare earth metal compound nano cluster and application thereof
  • Method for synthesizing rare earth metal compound nano cluster and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for synthesizing rare earth metal compound nanoclusters, comprising the following steps:

[0027] 1) The mixed solutions of europium nitrate and lanthanum chloride with different concentrations such as 1 / 17, 1 / 18, 1 / 19, 1 / 20, 1 / 21, 1 / 22, 1 / 23, etc. were cultured with liver cancer cells in cell culture 37°C in box with 5% CO 2 Co-incubated for 10 hours to synthesize rare earth metal compound nanoclusters;

[0028] 2) Quantitative analysis of rare earth metal compound nanoclusters with a fluorescence spectrometer;

[0029] Liver cancer cells (HepG2) were selected as the research object. In the experimental group, hepatic cancer cells (HepG2) in the logarithmic growth phase were inoculated in a 6-well plate at a density of 1.6×105 cells / well, and after 24 hours of culture, sterilized and fresh Mixed solutions of lanthanum chloride and europium nitrate diluted in sterile DMEM medium were incubated together for 10 hours in a cell culture incubator to synthesize na...

Embodiment 2

[0031] A method for synthesizing rare earth metal compound nanoclusters, comprising the following steps:

[0032] 1) Mix the mixed solution of lanthanum chloride and 0.01mg / ml europium nitrate with a concentration of 1mg / ml and liver cancer cells in a cell incubator at 37°C, containing 5% CO 2 Co-incubated for 10 hours to synthesize rare earth metal compound nanoclusters;

[0033] 2) Qualitative and quantitative analysis of rare earth metal compound nanoclusters with fluorescence spectrometer, confocal fluorescence microscope, etc.;

[0034] Liver cancer cells (HepG2) were selected as the research object. In the experimental group, hepatic cancer cells (HepG2) in the logarithmic growth phase were inoculated in a 6-well plate at a density of 1.6×105 cells / well, and after 24 hours of culture, sterilized and fresh A sterile DMEM medium was used to dilute a mixed solution containing 0.01 mg / ml lanthanum chloride and 1 mg / ml europium nitrate.

[0035] In the control group, liver ...

Embodiment 3

[0038] A method for synthesizing nano-clusters of rare earth metal compounds, characterized in that it comprises the following steps:

[0039] 1) Mix the mixed solution of lanthanum chloride and 0.01 mg / ml europium nitrate with a concentration of 1 mg / ml and breast cancer cells in a cell culture incubator at 37 ° C, containing 5% CO 2 Co-incubated for 24 hours to synthesize rare earth metal compound nanoclusters;

[0040] 2) Qualitative and quantitative analysis of rare earth metal compound nanoclusters with fluorescence spectrometer, confocal fluorescence microscope, etc.;

[0041] Construct a breast cancer model inoculated subcutaneously in nude mice. After breast cancer cells are cultured for 24 hours, add a mixed solution of lanthanum chloride and europium nitrate that has been sterilized and diluted with fresh sterile DMEM medium, and incubate in a cell culture box for 24 hours. Rare earth metal compound nanoclusters, after ultrasonically dispersing the solution containi...

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Abstract

The invention discloses a method for synthesizing a rare earth metal compound nano cluster and application of the rare earth metal compound nano cluster in the aspects of target imaging of diseased regions such as tumor and contrast medium preparation. The rare earth metal compound nano cluster prepared by the method has a small particle size, good permeability, high fluorescent stability, high sensibility and strong targeting, so that the tumor can be accurately positioned; the rare earth metal compound nano cluster is directly synthesized by biological tumor cells and has good biocompatibility; and the method is convenient to operate and intuitive in result, synchronous analysis and detection in multiple states and multiple modes can be carried out, and the method has a wide medical application prospect.

Description

technical field [0001] The invention relates to the field of synthesis and imaging of rare earth metal compound nanoclusters, in particular to a synthesis method and application of rare earth metal compound nanoclusters. Background technique [0002] With the development of modern science and technology, imaging technology has aroused widespread interest of scientists, and has been applied to the research of in vivo imaging and has achieved remarkable results. Hayashi et al. carried out a series of experiments using a small animal tumor model established by fluorescently labeled tumor cells and a real-time fluorescence imaging system. RobertMH may observe the process of tumor metastasis through in vivo fluorescent protein labeling of tumor cells and high-resolution small animal fluorescence imaging system. However, this technique still has many limitations, especially the tissue autofluorescence produced by in vivo illumination and the weak tissue permeability under the irr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/04A61K49/08A61K49/22
Inventor 王雪梅高生平李水红
Owner SOUTHEAST UNIV
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