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A kind of in-situ rapid synthesis of gold and silver fluorescent nanomaterials based on biomolecules such as nucleosides and its preparation method and application

A technology of fluorescent nanomaterials and nucleosides, which is applied in the in-situ rapid synthesis of gold and silver fluorescent nanomaterials based on nucleosides and other biomolecules and its preparation and application fields, which can solve the problem of increasing the concentration of modified nucleosides and accelerating the degradation of transfer RNA, etc. problem, to achieve the effect of intuitive results, high sensitivity, and intuitive detection

Active Publication Date: 2015-09-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the human body becomes cancerous, it will accelerate the degradation of transfer RNA, resulting in an increase in the concentration of modified nucleosides in urine

Method used

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  • A kind of in-situ rapid synthesis of gold and silver fluorescent nanomaterials based on biomolecules such as nucleosides and its preparation method and application
  • A kind of in-situ rapid synthesis of gold and silver fluorescent nanomaterials based on biomolecules such as nucleosides and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method for in-situ rapid synthesis of gold-silver fluorescent nanomaterials based on biomolecules such as nucleosides, prepared by the following steps:

[0038] In the first step, an aqueous solution of cytidine, gold reagent and silver reagent of a certain concentration is prepared.

[0039] In the second step, mix cytidine nucleoside and gold reagent according to the molar ratio of nucleoside to gold ion of 16:1, add sodium citrate solution to adjust the pH value to 6-7, fully mix the three, and then heat in a water bath or Leave at room temperature.

[0040] In the third step, after incubating at 37° C. for 3 hours or heating at 80° C. for 5 minutes, add silver reagent equal to the amount of gold reagent to the mixed solution, and cool to obtain a gold-silver nano-alloy with excellent fluorescence.

[0041] The gold reagent is chloroauric acid; the silver reagent is silver nitrate solution. The obtained material is tested under ultraviolet light, and th...

Embodiment 2

[0043] In the first step, an aqueous solution of cytidine biomolecule, gold reagent and silver reagent is prepared at a certain concentration.

[0044] In the second step, mix cytidine and gold reagent at a molar ratio of 16:1, add a certain amount of sodium citrate solution to adjust the pH value to 6-7, fully mix the three, and then heat in a water bath or place at room temperature.

[0045] In the third step, after incubating at 37°C for 3 hours or heating at 80°C for 5 minutes, add a silver reagent equivalent to the gold reagent to the mixed solution, and cool to obtain a gold-silver nano-alloy with excellent fluorescence; wherein the heating time, heating temperature, cell The ratio of pyrimidine nucleoside, gold reagent, and silver reagent can be adjusted, but the fluorescent colors of the obtained materials are different.

[0046] The gold reagent is tetragold octachloride; the silver reagent is silver nitrate solution.

Embodiment 3

[0048] In the first step, an aqueous solution of a certain concentration of cytidine, gold reagent and silver reagent is prepared.

[0049] In the second step, mix cytidine and gold reagent at a ratio of 16:1, add a certain amount of sodium citrate solution to adjust the pH value to 6-7, mix the three thoroughly, and then heat in a water bath or place at room temperature.

[0050] In the third step, after incubating at 37°C for 3 hours or heating at 80°C for 5 minutes, add a silver reagent equivalent to the gold reagent to the mixed solution, and cool to obtain a gold-silver nano-alloy with excellent fluorescence; wherein the heating time, heating temperature, cell The ratio of pyrimidine nucleoside, gold reagent, and silver reagent can be adjusted, but the fluorescent colors of the obtained materials are different.

[0051] The gold reagent is gold chloride; the silver reagent is silver nitrate solution.

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Abstract

　Disclosed in the present invention are a gold / silver fluorescent nanomaterial synthesized rapidly in-situ on the basis of biomolecules such as nucleosides, and a preparation method and a use for said material. In the invention, biomolecules such as nucleosides are used as a template for the rapid in-situ production of a gold / silver nanoalloy. The nanomaterial has excellent biocompatibility and stable fluorescence properties, is able to achieve highly-sensitive and rapid testing of the bodily fluid of both healthy people and patients having illnesses such as cancer, and facilitates diagnosis in respect of relevant physiological indicators. The method of the present invention allows for the detection of nucleosides and other related bio-substances in the bodily fluids of patients such as cancer patients. The invention thereby facilitates rapid, highly-sensitive, low-cost, and directly-observable testing for physiological indicators of patients such as cancer patients. The method is easy to apply, low in toxicity, highly sensitive, and high in specificity. Real-time, dynamic clinical testing can be implemented in multiple forms and modes, giving the invention great value and a wide range of prospects for medical / clinical application.

Description

technical field [0001] The invention relates to a preparation method for in-situ rapid synthesis of gold-silver fluorescent nanomaterials based on biomolecules such as nucleosides and its application in the detection of body fluids of patients with diseases such as cancer. It specifically relates to the preparation of gold-silver nano-alloy materials with enhanced fluorescence using nucleosides and other biomolecules as templates, and the application of this method in the preparation of test kits for the detection of body fluids of patients with cancer and other diseases. Background technique [0002] As we all know, the early symptoms of cancer are not obvious, and it is difficult to be detected in time, and the current conventional histopathological diagnosis method is costly, redundant, and difficult to detect the biochemical and molecular abnormalities of diseased tissue and cells in the early stage. Fast and accurate early cancer diagnosis methods are of great significa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52G01N21/64C09K11/58
CPCG01N21/64C09K11/58B82Y20/00G01N33/52B82Y40/00G01N33/533G01N33/553G01N33/587
Inventor 王雪梅张园园姜晖革伟
Owner SOUTHEAST UNIV
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