CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit

A diagnostic kit and breast cancer technology, applied in the field of biomedicine, can solve the problems of easy misdiagnosis or missed diagnosis, low detection sensitivity and specificity, and difficulty in detecting patients with early or recurrent breast cancer.

Active Publication Date: 2008-09-03
BEIJING MOKOBIO LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the single detection of one of the above tumor markers cannot provide more information, and the detection sensitivity and specificity are relatively low. It is difficult to detect patients with early or recurrent breast cancer, and misdiagnosis or missed diagnosis is prone to occur.

Method used

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  • CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit
  • CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit
  • CA15-3, CEA, CA19-9, CA12-5, SF mammary cancer colloidal gold five joint inspection diagnostic reagent kit

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Embodiment 1

[0030] Example 1 Preparation of colloidal gold five-joint detection diagnostic kit of the present invention

[0031] 1. Preparation of monoclonal antibodies against CA15-3, CEA, CA19-9, CA12-5, SF

[0032] BALB was treated with CA15-3, CEA, CA19-9, CA12-5, SF (CEA antibody was purchased from Tianjin Tianjian Biopharmaceutical Co., Ltd., SF antibody was purchased from Medix Biochemica, CA125, CA153, and CA199 were purchased from CanAg Diagnostics AB). / c Pure line mice are immunized, and when the mouse serum produces corresponding antibodies, the splenocytes and SP2 / 0 cells can be fused. Hybridoma cells were selected by HAT, and McAb was detected by enzyme-linked immunosorbent assay (ELISA). For hybridomas positive for antibody detection, the limited dilution method should be used for cloning, and the positive cell lines should be preserved at the same time, and a large number of monoclonal antibodies should be prepared by using BALB / c pure-line mice. Affinity identification ...

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Abstract

The invention discloses a diagnosis kit for breast cancer and preparation method thereof. Five kinds of tumor markers for diagnosis of breast cancer such as CA15-3, CEA, CA19-9, CA12-5, SF are detected together at the same test paper and using immunity chromatography technique the monoclonal antibodies for CA15-3, CEA, CA19-9, CA12-5, SF are fixed on the cellulose nitrate film, at the same time the five kinds of monoclonal antibodies are marked by gold particles of different size and the said gold antibodies are mixed to produce gold affixture pad, finally the gold affixture pad is assembled with a sample treating pad to form a test paper. The said kit quickly test the five kinds of antigens in human whole blood or serum, plasm using double antibody sandwiching method, so as to conquer many defects of previously separation detecting method, only one drop of blood can detect five tumor markers and the detection result is more direct and the sensitivity and specificity of detection is greatly increased.

Description

technical field [0001] The present invention relates to a kit for diagnosing tumors, in particular to a diagnostic kit for colloidal gold five-point detection of breast cancer. The present invention also relates to the preparation method of the kit and the application of the kit in diagnosing breast cancer, belonging to biomedicine field. Background technique [0002] Breast cancer is the most common epithelial cell tumor in the world, poses a major threat to human health and survival, and is one of the most important social problems facing countries all over the world. Today, breast cancer accounts for 7%-10% of systemic malignant tumors, and its incidence has been increasing year by year, with an annual growth rate of 3% since 1980. Especially in North America, Western Europe and other developed countries, it is particularly obvious. About 1.2 million women develop breast cancer every year in the world, and 500,000 women die of breast cancer. 2006 American Cancer Societ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/558
Inventor 金鑫戴路
Owner BEIJING MOKOBIO LIFE SCI CO LTD
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