19results about "Specific reaction combinations" patented technology

Apparatus is provided and an IDA method is modified to detect and separately dissociate alkali-metal adducts of a compound. An ion source device ionizes one or more compounds of a sample, producing an ion beam. A mass filter selects a mass range of precursor ions from the ion beam, a mass analyzer measures intensities and m / z values of the precursor ions, and one or more of the precursor ions are selected for a peak list. For each pair of precursor ions of the peak list, if an m / z difference between the pair corresponds to an m / z difference between an alkali metal ion and another alkali metal ion or a proton, an ExD device is used to dissociate one precursor ion or both precursor ions of the pair using the processor. A CID device is used to dissociate all other precursor ions of the peak list.

A mass spectrometry method comprises: choosing an RF drive frequency that best optimizes isolation of a precursor ion species of interest by a quadrupole mass filter (QMF); isolating the precursor ion species by passing ions through the QMF while the chosen RF drive frequency is applied thereto; fragmenting the precursor ion species, thereby generating a plurality of first-generation fragment ion species; returning the plurality of first-generation fragment ion species to an inlet end of the QMF; choosing a second quadrupole RF drive frequency that best optimizes isolation of a first-generation fragment ion species of interest by the QMF; isolating the first-generation fragment ion species of interest by passing the fragment ions through the QMF while the second chosen RF drive frequency is applied thereto; fragmenting the chosen first-generation fragment ion species of interest, thereby generating a plurality of second-generation fragment ion species; and mass analyzing the second-generation fragment ion species.

A method for determining a combined amount of β-tocopherol and γ-tocopherol in a sample by tandem mass spectrometry includes subjecting the sample to ionization under conditions suitable to produce one or more ions from the β-tocopherol and γ-tocopherol detectable by mass spectrometry; and determining the amount of one or more of the ions by tandem mass spectrometry. Tandem mass spectrometry methods of this disclosure involve fragmenting β-tocopherol and γ-tocopherol ions having a mass to charge ratio of about 416.35±0.80 into one or more fragment ions including a fragment ion having a mass to charge ratio of about 151.00±0.80, wherein the amount of the one or more ions determined is related to the combined amount of β-tocopherol and γ-tocopherol in the sample.

The present invention relates to a method for determining an analyte in a mass spectrometer (MS) apparatus comprising first and second mass filters, the method comprising: (i) filtering an analyte ion species in a first mass filter; (ii) optionally fragmenting at least a portion of the ions obtained by filtering in step (i) in a collision cell, wherein the collision energy of the fragmentation is selected to be lower than a predetermined collision energy that causes the fragmentation of the analyte ion species; (iii) filtering the analyte ion species filtered in step (i) in a second mass filter; and (iv) detecting the analyte ion species filtered in step (iii) to determine the analyte. Furthermore, the present invention relates to apparatus, systems, and uses related to the method.

The invention relates to a method for analyzing a sample comprising one or more molecules of interest by a mass spectrometer, comprising ionizing a multitude of molecules and / or fragments thereof comprised within a sample to obtain a multitude of molecule ions (source ions), and performing mass spectrometry (MS) analysis comprising a. analyzing the molecule ions and / or fragments thereof in a MS1 measurement, thereby acquiring ion mass spectral data of at least a fraction of the molecule ions and / or fragments thereof (source ions) comprising their mass to charge ratio (m / z), b. / c. isolating a first fraction and a second of the molecule ions (precursor ions) having a m / z within a first m / z range (mz1; isolation window) and a second m / z range (mz2; isolation window), using a mass filter device, wherein the first m / z range is selected based on the ion mass spectral data acquired in step a. or one or more iterations of step a. and / or e., and wherein the second m / z range (mz2) overlaps with the first m / z range (mz1), d. fragmenting at least a fraction of the molecule ions (precursor ions) isolated in steps b. and c., separately, to obtain a multitude of biomolecule fragment ions from each of the respective fragmented molecule ions (precursor ions), and e. analyzing the multitude of biomolecule fragment ions in one or more MS2 measurement, thereby acquiring ion mass spectral data of the analyzed biomolecule fragment ions.

Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution / high accuracy mass spectrometric techniques.

Disclosed herein is a method of analyzing a sample by mass spectrometry. A precursor is acquired from the sample. At least one mass spectrum associated with the precursor is generated based on performing a first level of analysis on the precursor. At least one motif in the precursor is identified based on performing a real-time error-tolerant search of the at least one mass spectrum using stored mass spectral reference data. Based on identifying the at least one motif in the precursor, at least one second level of analysis on the at least one motif is initiated to determine an identity of the precursor.

The precursor ion is reacted with ammonia molecules or ammonia radicals to generate product ions, the product ions are separated according to a mass-to-charge ratio, and detected. It is estimated that whether or not an aldehyde group is included in a molecular structure of the precursor ion based on a difference between a mass-to-charge ratio of one of the detected product ion and a mass-to-charge ratio of the precursor ion. In addition, provided is a mass spectrometer (1) including: a reaction chamber (132) to which the precursor ions are introduced; an ammonia supply part (5) configured to supply ammonia molecules or ammonia radicals to the reaction chamber; and separation and detection parts (142, 143, 144, and 145) configured to separate product ions generated from the precursor ion by reaction with the ammonia molecules or ammonia radicals according to a mass-to-charge ratio, and to detect the product ions.

A mass spectrometer including: a reaction chamber into which a precursor ion derived from a sample molecule is introduced; a collision gas supply part configured to supply collision gas to the reaction chamber; a radical supply part configured to supply hydrogen radicals, oxygen radicals, nitrogen radicals, or hydroxyl radicals to the reaction chamber; a dissociation operation control part configured to control operations of the collision gas supply part and the radical supply part to generate the product ions by collision-induced dissociation and radical attachment dissociation of the precursor ion inside the reaction chamber, an ion detection part configured to mass-separate and detect ions ejected from the reaction chamber, and a spectrum data generation part configured to generate spectrum data based on a detection result by the ion detection part.

Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution / high accuracy mass spectrometric techniques.

Method for operating an analytical instrument comprising a first ion storage and a second ion storage downstream of the first ion storage, wherein the method comprises: Determine whether a target accumulation time for the second ion storage is greater than a threshold accumulation time; If it is determined that the target accumulation time is less than the threshold accumulation time: accumulation of ions within the second ion storage using an accumulation time based on the target accumulation time; and If it is determined that the target accumulation time is greater than the threshold accumulation time: Accumulate ions within the first ion storage using a first accumulation time based on a difference between the target accumulation time and the threshold accumulation time, transfer the ions accumulated in the first ion storage to the second ion storage, and accumulate further ions within the second ion storage using a second accumulation time based on the threshold accumulation time.

A method of calibrating a mass spectrometer comprises generating calibration ions from an ion source that are transported from the ion source to a mass analyser of the mass spectrometer via an ion optics device. A characteristic voltage is applied to the ion optics device to control the transit of ions into or out of the ion optics device. The applied characteristic voltage results in an amount of unintentional dissociation of the calibration ions. An MS1 analysis of the calibration ions is performed using the mass analyser to obtain an MS1 spectrum. The amount of unintentional dissociation of the calibration ions in the MS1 spectrum is determined. The characteristic voltage is calibrated based on the amount of unintentional dissociation of the calibration ions to provide a target amount of unintentional dissociation during an MS1 analysis.

To prevent occurrence of unnecessary time between two cycle measurement sections temporally adjacent to each other in a mass spectrometer.SOLUTION: A plurality of measurement sections Sa1-Sa5 are set on a retention time axis on the basis of a plurality of compound peak observation periods. The starting time and the ending time of each of the measurement sections Sa1-Sa5 are corrected so as to prevent occurrence of a blank period (remainder time) r1-r5 between two measurement sections temporally adjacent to each other, and thereby the actual starting time and the actual ending time of each of the measurement sections Sb1-Sb5 are determined. Specifically, the actual starting time of the i-th cycle measurement section is adjusted to the actual ending time of the i-1-th cycle measurement section.SELECTED DRAWING: Figure 5

A method of operating a time-of-flight (TOF) mass analyser is disclosed, comprising injecting first and second ions into an ion path, causing the first ions to travel from an ion reflector 42b to a de

A method for determining a combined amount of β-tocopherol and γ-tocopherol in a sample by tandem mass spectrometry includes subjecting the sample to ionization under conditions suitable to produce one or more ions from the β-tocopherol and γ-tocopherol detectable by mass spectrometry; and determining the amount of one or more of said ions by tandem mass spectrometry, wherein tandem mass spectrometry comprises fragmenting β-tocopherol and γ-tocopherol ions having a mass to charge ratio of about 416.35±0.80 into one or more fragment ions comprising a fragment ion having a mass to charge ratio of about 151.00±0.80, wherein the amount of the one or more ions determined is related to the combined amount of β-tocopherol and γ-tocopherol in the sample.

The invention relates to a method for analyzing a sample comprising one or more molecules of interest by a mass spectrometer, comprising ionizing a multitude of molecules and / or fragments thereof comprised within a sample to obtain a multitude of molecule ions (source ions), and performing mass spectrometry (MS) analysis comprising a. analyzing the molecule ions and / or fragments thereof in a MS1 measurement, thereby acquiring ion mass spectral data of at least a fraction of the molecule ions and / or fragments thereof (source ions) comprising their mass to charge ratio (m / z), b. / c. isolating a first fraction and a second of the molecule ions (precursor ions) having a m / z within a first m / z range (mz1; isolation window) and a second m / z range (mz2; isolation window), using a mass filter device, wherein the first m / z range is selected based on the ion mass spectral data acquired in step a. or one or more iterations of step a. and / or e., and wherein the second m / z range (mz2) overlaps with the first m / z range (mz1), d. fragmenting at least a fraction of the molecule ions (precursor ions) isolated in steps b. and c., separately, to obtain a multitude of biomolecule fragment ions from each of the respective fragmented molecule ions (precursor ions), and e. analyzing the multitude of biomolecule fragment ions in one or more MS2 measurement, thereby acquiring ion mass spectral data of the analyzed biomolecule fragment ions.

A method of analysing ions is disclosed comprising: (i) subjecting ions of an analyte molecule to different activation levels at different times so as to cause the ions to have different mobilities at said different times, wherein the activation level is varied in a plurality of cycles, and wherein the activation level is varied between said different levels during each of the cycles. The method uses an ion mobility separator or scanned ion mobility filter to determine the mobilities of the ions for said different activation levels; and correlates the determined mobilities with their respective activation levels so as to thereby obtain a fingerprint for the analyte molecule.