142 results about "Mass separation" patented technology

This invention is based on size and mass separation of suspended particles, including biological matter, which are made to flow in a spiral channel. On the spiral sections, the inward directed transverse pressure field from fluid shear competes with the outward directed centrifugal force to allow for separation of particles. At high velocity, centrifugal force dominates and particles move outward. At low velocities, transverse pressure dominates and the particles move inward. The magnitudes of the two opposing forces depend on flow velocity, particle size, radius of curvature of the spiral section, channel dimensions, and viscosity of the fluid. At the end of the spiral channel, a parallel array of outlets collects separated particles. For any particle size, the required channel dimension is determined by estimating the transit time to reach the side-wall. This time is a function of flow velocity, channel width, viscosity, and radius of curvature. Larger particles may reach the channel wall earlier than the smaller particles which need more time to reach the side wall. Thus a spiral channel may be envisioned by placing multiple outlets along the channel. This technique is inherently scalable over a large size range from sub-millimeter down to 1 μm.

Ion manipulation systems include ion repulsion by an RF field penetrating through a mesh. Another comprises trapping ions in a symmetric RF field around a mesh. The system uses macroscopic parts, or readily available fine meshes, or miniaturized devices made by MEMS, or flexible PCB methods. One application is ion transfer from gaseous ion sources with focusing at intermediate and elevated gas pressures. Another application is the formation of pulsed ion packets for TOF MS within trap array. Such trapping is preferably accompanied by pulsed switching of RF field and by gas pulses, preferably formed by pulsed vapor desorption. Ion guidance, ion flow manipulation, trapping, preparation of pulsed ion packets, confining ions during fragmentation or exposure to ion to particle reactions and for mass separation are disclosed. Ion chromatography employs an ion passage within a gas flow and through a set of multiple traps with a mass dependent well depth.

The content of the invention comprises a concept of multi-beam ion pre-selection from a single sample, coordinated mobility (against the gas flow) separation, cooling ions in supersonic gas flow and mass separation of thus low divergent ions by single or plural compact high-resolution orthogonal time-of-flight mass spectrometers both linear or reflectron type with controlled collision-induced dissociation (CID) and multi-channel data recording for the optimization of sample use in the analysis, and obtaining as much useful information about the sample as possible in a reasonably short time.

A mass analysis of a standard sample having a known mass-to-charge ratio is carried out by performing a mass scan at a first-stage quadrupole (13) over a predetermined mass range, under the condition that a collision induced dissociation (CID) gas is introduced into a collision cell (14) and a voltage applied to a third-stage quadrupole (17) is set so that no substantial mass separation occurs in this quadrupole. Various kinds of product ions originating from a precursor ion selected by the first-stage quadrupole (13) arrive at and are detected by a detector (18) without being mass separated. Accordingly, based on the detection data, a data processor (25) can obtain a relationship between the voltage applied to the first-stage quadrupole (13) and the mass-to-charge ratio of the selected ions, with a time delay in the collision cell (14) reflected in that relationship. This relationship is stored in a calibration data memory (26), to be utilized in a neutral loss scan measurement or the like. By using this relationship, a mass shift due to the time delay in the collision cell (14) can be cancelled, so that the product ions can be detected with high sensitivity over the entire mass range. Furthermore, a mass spectrum having an accurate mass axis can be created.

A method of analysing ions is disclosed comprising performing an initial multi-dimensional survey scan comprising separating parent ions according to a first physico-chemical property (e.g. ion mobility) and then separating the parent ions according to a second physico-chemical property (e.g. mass to charge ratio). A plurality of parent ions of interest are then determined from the initial multi-dimensional survey scan. Once parent ions of interest have been determined, the plurality of parent ions of interest are sequentially selected based upon the first and second physico-chemical properties during a single cycle of separation. The parent ions of interest may then be fragmented and corresponding fragment ions may then be mass analysed.

While applying a square wave voltage to the ion electrode (21) so that ions already captured in the ion trap (20) do not disperse, the frequency of the square wave voltage is temporarily increased at the timing when the ions generated in response to the short time irradiation of a laser light reach the ion inlet (25). This decreases the Mathieu parameter q_z, and the potential well becomes shallow, which makes it easy for ions to enter the ion trap (20). Although the ions that have been already captured become more likely to disperse, the frequency of the square wave voltage is decreased before they deviate from the stable orbit. Thus, the dispersion of the ions can also be avoided. Accordingly, while the number of captured ions is not decreased, new ions are further added, and thereby the amount of ions can be increased. By performing a mass separation and detection after that, the signal intensity in one mass analysis can be increased. Thereby, the number of repetition of the mass analysis for summing up the mass profiles can be decreased, and the signal intensity can be increased while decreasing the measuring time.

A novel method and apparatus for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS). The FTICR-MS apparatus has a pre-ICR mass separation and filtering device capable of receiving ionized molecules with a plurality of mass to charge (M/Z) sub-ranges. The pre-ICR mass separation and filtering device divides the ionized molecules into a plurality of smaller packets, each of the smaller packets is within one of the M/Z sub-ranges. A magnet in the FTICR-MS apparatus provides a controlled magnetic field. A plurality of ion cyclotron resonance (ICR) cells are arranged in series in the controlled magnetic field and operate independently. An ion trapping device connects the pre-ICR mass separation and filtering device, and stores one of the plurality of smaller packets, prior to sending it to one of the plurality of ICR cells.

An ion beam apparatus comprises an ion source 2 which extracts an ion beam 4, a mass separation electromagnet 6 which separates an ion beam 4 of desired mass from the ion beam 4 extracted from the ion source 2, a scanner 12 which scans the injected ion beam 4 with a given scan center as center within a given scan surface, an electrostatic deflector 30 which deflects the ion beam through 90° so that an ion beam of desired energy in said ion beam travels in a direction perpendicular to said scan surface within a circular-arc-shaped deflection zone centered on the scan center, and a scanning mechanism 54 which retains a target 50 and which mechanically, reciprocally moves the target 54 in a direction in which the target crosses the ion beam passed from the electrostatic deflector 30 at a given angle.

A gas chromatography/mass spectrometry system includes two sample introduction parts, a gas chromatography part having two columns, a column oven for housing the two columns in a parallel configuration, an interface part through which an outlet end of each of the two columns is inserted, and a mass spectrometry part. The mass spectrometry part has an ionization chamber to which the outlet ends of the columns inserted through the interface part are connected, a mass separation part, an ion detector, and a vacuum chamber for housing the ionization chamber, the mass separation part, and the detector. Because the outlet ends of the parallel columns are both connected to the ionization chamber, the carrier gas flow and line velocity are the same as though there is one column, thereby avoiding line stagnation. Since analyses can be performed selectively with a single system, results having high reliability and precision can be obtained.

An aerosol TOF MS of the present invention is based on the use of quadrupole lenses with angular gradient of the electrostatic field. On the entrance side, the TOF MS contains an ion-optic system that is used for focusing, aligning, and time-modulating the ionized flow of particles and a deflector modulator that provides alternating deflections of the flow of particles between two positions for aligning the flow with two inlet openings into the TOF MS. As a result, two independently analyzed discrete flows of particles pass through the ion mass separation chamber of the TOF MS without interference with each other. The charged particles fly in direct and return paths along helical trajectories which extend the length of the flight time. The time of the collision and the magnitude of the collision pulse will contain information about the M/Z ratio for the particles being registered. Multiplication of a single flow of particles into a plurality of independent and spatially separated flows propagating in one chamber increases efficiency of the TOF MS and makes it possible to use it in continuous and high-duty applications with the duty cycle as high as 98%, which is unattainable with any known device of this class.

The invention discloses an umbilical cord mesenchymal stem cell (MSC) separation culture and amplification method. The umbilical cord MSC separation culture and amplification method comprises the steps of (1) clearing, (2) treatment, (3) culture, and (4) inoculation and passage. The umbilical cord MSC method obtained by adopting a tissue mass separation method is simple, the cost is low, cell activity is good, purity is high, the final harvested cell concentration is high, the obtained MSC method is simple, MSCs can be quickly and efficiently separated, the culture time is shortened, after culture is conducted for 4-5 days, it can be seen that the cells climb out from tissue masses, the final harvested cell concentration is high, and the entire umbilical cord MSC culture process can be standardized, programmed and normalized.

This invention relates to a system and process for recovering and recycling valuable target recycle materials such lost circulation material and weighting agents from drilling fluid used in hydrocarbon drilling operations. The system and process includes use of a binary fluid that is separate from the drilling fluid and has a designed density that allows separation of the valuable target recycle materials from drill cuttings. The solids are separated from the drilling fluid in a primary separation step and then mixed with the binary fluid in a density separation or enhanced mass separation device. The binary fluid density allows the separation device to distinguish the target recycle material from drilling solids and recycles the target material to the drilling operations for re-use in drilling fluid. The binary fluid is recovered and refreshed to maintain a generally continuous process of targeted recycling.

A mass spectrometer has an ionizing portion for ionizing a sample, a mass spectrometric portion for subjecting a sample ion to mass separation and detecting the sample ion and a control portion for controlling operation of a total of the apparatus. The mass spectrometer is provided with a colliding portion provided at an ion path until a sample ion generated by the ionizing portion is introduced to amass spectrometric portion and a gas introducing portion for introducing one kind or more of gases selected from two kinds or more of gases into the colliding portion. By carrying out analysis by using the apparatus, highly accurate mass spectrometry can be carried out.

The present invention provides a method for manufacturing an SOI substrate, to improve planarity of a surface of a single crystal semiconductor layer after separation by favorably separating a single crystal semiconductor substrate even in the case where a non-mass-separation type ion irradiation method is used, and to improve planarity of a surface of a single crystal semiconductor layer after separation as well as to improve throughput. The method includes the steps of irradiating a single crystal semiconductor substrate with accelerated ions by an ion doping method while the single crystal semiconductor substrate is cooled to form an embrittled region in the single crystal semiconductor substrate; bonding the single crystal semiconductor substrate and a base substrate with an insulating layer interposed therebetween; and separating the single crystal semiconductor substrate along the embrittled region to form a single crystal semiconductor layer over the base substrate with the insulating layer interposed therebetween.

The invention relates to a single spore isolation and purification method of verticillium dahliae. The method mainly comprises the following steps: an agar medium cylinder is taken from a water agar plate by the use of a glass capillary with diameter being 1mm and is picked onto a sterilized glass slide with a sterilization needle; a verticillium dahliae strain is activated in a PDA solid medium and the activated strain is cultured in a Czapek-Dox liquid medium; spore concentration is counted under a microscope; and the spore concentration is adjusted to 102-106 cfu to prepare a spore suspension; 1 microlitre of the spore suspension is sucked up and dripped onto the surface of the water agar cylinder on the glass slide; the glass slide is moved into a culture dish for constant temperature incubation at 25 DEG C; the glass slide is detected under the microscope, and the cylinder containing a single conidiospore which has germinated germ tubes is transferred onto a PDA plate to be cultured at 25 DEG C so as to finish single spore isolation. The method is simple to operate, can be implemented by general operation staff and is suitable for mass separation. By the method, separation success rate is high. The method is also suitable for production of other fungi of microconidia.

The present invention employs a mass analyzer comprised of a pair of permanent magnets to select a desired species from multiple species within a ribbon type ion beam. These permanent magnets provide a substantially uniform magnetic field of adequate magnitude in a small region not attainable with electromagnets that applies a specific force in a desired direction. The force is applied to passing particles of a ribbon ion beam and causes paths of the particles to alter according to their respective mass. As a result, a selected species can be obtained from a beam by the force causing rejected species and/or contaminants to fail passing through the mass analyzer (e.g., by impacting the magnets themselves and/or another barrier present in the analyzer). As a result of the mass analyzer, dopant/species sources that generate multiple species can be employed instead of sources that only supply a single dopant/species.

An object of the present invention is to provide a mass spectrometer, a method of mass spectrometry, and a program for mass spectrometry for narrowing the range in which the mass-to-charge ratio is scanned without the ion peak of the fragment ion becoming out of the range. In order to achieve the above object, a mass spectrometer including a control unit, a display unit provided with an user interface, an ionization chamber, a dissociation chamber, a mass separator, and a detector is provided.