Method and device for producing ions used for analysis under low pressure

An ion and ion focusing technology, applied in the parts of the particle separator tube, particle separation tube, ion source/gun, etc., can solve the problems of loss and insufficient sensitivity of the ion source, achieve high sensitivity, increase the application range, improve the The effect of sensitivity

Active Publication Date: 2013-09-11
SHIMADZU SEISAKUSHO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even for neutral molecules, there are still losses at the vacuum interface, and ion source sensitivity is still not high enough

Method used

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  • Method and device for producing ions used for analysis under low pressure
  • Method and device for producing ions used for analysis under low pressure
  • Method and device for producing ions used for analysis under low pressure

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Such as figure 1 Shown is an example according to the first embodiment of the present invention, in which 1 is the vacuum cavity where the ion source is located, and the electrospray needle 2 generates electrospray after applying high voltage. The electrospray needle is a nanoliter spray tip. The direction of the spray needle is perpendicular to the transport direction of the ion funnel. After necessary desolvation measures, the charged droplets generated by the electrospray pass through the ion focusing device (the ion funnel 8, the Q-array guide 12 and the octopole rod 13 in sequence), and then enter the analyzer for analysis. The sample stage 3 and the sample holder 4 placed on it together form a sample platform. The sample stage 3 is connected to the mechanical device and is controlled to move, and under the condition of not breaking the vacuum of the cavity, the sample stage 3 and the sample holder 4 pass through the vacuum together. The introduction device 5 mov...

Embodiment 2

[0056] figure 2 This is a modified example of the first embodiment of the present invention. On the basis of the original embodiment, this modification adds a flange 14 with a small hole, the flange is located between the low-pressure spray needle and the matrix-assisted laser desorption ionization area, and the suction port of the mechanical pump is still located in the area of ​​the laser desorption ionization area. In the region, a differential pumping step more than that in Embodiment 1 is formed, so that the two ionization sources work more suitable for different pressure ranges.

Embodiment 3

[0058] image 3 This is another modified example of the first embodiment of the present invention. In this modification, the ion funnel 8 in the previous modification is moved to the cavity of the needle and used to replace the small hole 14 of the flange. At this time, the electrospray works at 10-30 torr, and the air pressure inside the vacuum chamber 15 where matrix-assisted laser desorption ionization works is 1-2 torr or even lower.

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Abstract

The invention relates to a method and device for producing ions used for analysis, in particular to an ionization method and device for producing ions through multiple modes under the lower pressure and enabling multiple ion sources to be compounded and used. According to the method and device, an electrospray ion source and other desorbing / ionization devices (such as laser desorbing ion sources) are placed in the same vacuum cavity and act on sample molecules in the vacuum cavity independently or commonly, and the irons produced by ionization are fed into an ion analyzer to be analyzed through an ion focusing device.

Description

technical field [0001] The invention relates to a method and device for generating ions for analysis, especially an ionization method and device for generating ions in multiple ways under relatively low pressure and using the multiple ion sources in combination. Background technique [0002] For mass spectrometry analysis of macromolecules (such as proteins, peptides, and DNA molecules), softer ionization methods are usually required. The ionization methods currently used in this field generally include: electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI). These two ionization methods have their own advantages and disadvantages. For example, ESI can generate multi-charged ions, and with high-resolution mass spectrometry, it can conveniently characterize macromolecules. Therefore, ESI sources have been widely used at present, but ESI generally cannot meet the requirements of high ionization at the same time. Throughput and high sensitivity; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/16
CPCH01J49/0463H01J49/14H01J49/16
Inventor 张小强孙文剑
Owner SHIMADZU SEISAKUSHO CO LTD
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