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Application for analyzing micromolecules with irradiating nanometer carbon spots serving as matrix assisted laser desorption ionization (MALDI) matrix

A technology of nano carbon dots and small molecules, which is used in the analysis of materials, material analysis by electromagnetic means, measurement devices, etc., can solve problems such as instrument damage, low sensitivity, and reduced analysis effect, and achieve reduction requirements and sample compatibility. Good results

Inactive Publication Date: 2012-08-22
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Even so, the above methods still have deficiencies. For example, when desorption / ionization occurs on the porous silicon surface, the surface of the silicon crystal must first be etched to produce a nanostructured surface. Although peptides and antibodies can be directly analyzed on such a surface. Viral drugs, but since the reusability of this surface is not good, repeated treatments on the same nanostructured surface are required to obtain a reusable surface
In addition, it takes a long time to chemically modify the surface of porous silicon, and the analysis effect will be greatly reduced when the modified porous silicon is reused; for the previous carbon materials, graphite, fullerene and carbon nanotubes are poor in water solubility, and When used as a substrate, it often causes high-voltage discharges in the mass spectrometer due to its high conductivity, which can cause damage to the instrument; and graphene itself has strong adhesion on the MALDI target, and will have a strong adsorption on small molecules, so Not very sensitive when analyzing some substances

Method used

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  • Application for analyzing micromolecules with irradiating nanometer carbon spots serving as matrix assisted laser desorption ionization (MALDI) matrix
  • Application for analyzing micromolecules with irradiating nanometer carbon spots serving as matrix assisted laser desorption ionization (MALDI) matrix
  • Application for analyzing micromolecules with irradiating nanometer carbon spots serving as matrix assisted laser desorption ionization (MALDI) matrix

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1. Analysis of various small molecules (including amino acids, small peptides, β-agonists, fatty acids, polymers, etc.)

[0039] Take various prepared sample solutions (concentration of 1mM) and matrix (carbon dots) solution (concentration of 1mg / mL) and mix them uniformly at a volume ratio of 1:1, then add 1 μL of the mixed sample to the MALDI target plate, and dry in the air Enter mass spectrometry. The mass spectrometry conditions are: voltage: accelerating voltage: 19.000kv; delayed extraction voltage: 14.920kv; reflector voltage: 20.000kv; lens voltage: 7.000kv; frequency: 1.000Hz; laser energy: 75-80%; accumulation times: 90 times ; positive or negative ion mode.

[0040] In the MALDI ionization mode, the matrix can absorb the laser energy and transfer it to the analyte to ionize the analyte. In the positive ion mode, protonation and alkali metal addition peaks can be formed, and in the negative ion mode, a deprotonation peak can be formed, which can be d...

Embodiment 2

[0041] Embodiment 2, the analysis of uric acid in the urine sample

[0042] Take 2 mL of fresh urine from healthy male volunteers without any sample pretreatment, then take 1 μL and mix it with 1 μL carbon-based (particle) solution (concentration: 1 mg / mL), and finally take 1 μL of the mixed solution and add it to the MALDI target plate. After drying in air, it was subjected to mass spectrometry analysis. The mass spectrometry conditions are: voltage: accelerating voltage: 19.000kv; delayed extraction voltage: 14.920kv; reflector voltage: 20.000kv; lens voltage: 7.000kv; frequency: 1.000Hz; laser energy: 75-80%; accumulation times: 90 times ; Negative ion mode.

[0043] From Figure 7 It can be seen from the figure that in the negative ion mode, except for uric acid molecules, other small molecules have no signal peaks, which may be because the content of other substances that can peak in the negative ion mode is very low, and no mass spectrum signal is generated under the c...

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Abstract

The invention discloses application for analyzing micromolecules with irradiating nanometer carbon spots serving as a matrix assisted laser desorption ionization (MALDI) matrix. The carbon spots serve as an analysis method of the MALDI matrix and are suitable to mass spectrum analysis for the micromolecules with the m / z between 150-1000. Suitable molecule types comprise amino acid, polypeptide, beta-exhilarant, fatty acid, polymer and the like. When the method is used for detecting the molecule with the m / z between 150-1000, background peaks hardly exist to produce background interference. The method can be effectively used in the fields of organic and biomass spectrometry, spectrometry imaging, protein spectroscopy, metabonomics, biomarker discovery, environment analysis and the like.

Description

technical field [0001] The invention relates to the application of luminescent nanometer carbon dots as a MALDI matrix to analyze small molecules. Background technique [0002] Since Karas et al. and Tanaka et al. reported in 1988 that matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology can be used for effective mass spectrometry analysis of biological macromolecules, MALDI-TOF MS technology has been favored by researchers from various countries. of favor. However, due to the commonly used matrices of MALDI-TOF MS technology, such as α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), sinapinic acid (SA), 3-hydroxy-2- Picolinic acid (3-HPA), anthracenol (DI) and 3-aminoquinoline (3-AQ) will be fragmented during the analysis process and the association between molecules will be in the range of m / z<1000 Severe matrix background interference occurs in the matrix, therefore, small molecule compounds cann...

Claims

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Application Information

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IPC IPC(8): G01N27/64
Inventor 陈素明王佳宁侯剑何清聂宗秀
Owner INST OF CHEM CHINESE ACAD OF SCI
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