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Application of silver nanowire material as MALDI-TOF MS matrix in metabolite detection

A technology of silver nanowires and metabolites, applied in the field of mass spectrometry detection, can solve the problems of matrix background interference, organic small molecule matrix rupture and association, difficult analysis, etc., to improve detection sensitivity, overcome matrix background interference phenomenon, and simplify detection. effect of difficulty

Inactive Publication Date: 2020-07-17
SICHUAN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although there are many types of organic small molecule matrices used in MALDI-TOF MS, it is difficult to analyze small molecular weight (less than 10,000 Da) compounds. Severe matrix background interference phenomenon

Method used

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  • Application of silver nanowire material as MALDI-TOF MS matrix in metabolite detection
  • Application of silver nanowire material as MALDI-TOF MS matrix in metabolite detection
  • Application of silver nanowire material as MALDI-TOF MS matrix in metabolite detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 prepares silver nanowire material

[0023] 0.170g AgNO 3 and 10 mL of ethylene glycol were dissolved under magnetic stirring. Then add 0.15M PVP-40 and 0.111mM CuCl dropwise 2 2H 2 After O(170.48) mixed solution (dissolved in 10 mL ethylene glycol), the mixture was transferred to a stainless steel autoclave (50 mL capacity) and heated at 160 °C for 3 h. After naturally cooling to room temperature, centrifuge at 2500r / min for 5 minutes, wash with ethanol and deionized water three times, and disperse the product in ethanol for characterization and application.

[0024] Scanning electron microscope (SEM) and ultraviolet-visible light (UV-vis) detection are carried out to the above-mentioned silver nanowire material that makes, and detection result sees Figure 1-2 .

[0025] in, figure 1 It is a scanning electron microscope (SEM) picture of silver nanowire material; figure 2 It is the UV-Vis absorption spectrum of silver nanowire material.

[0026] De...

Embodiment 2

[0028] Example 2 Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Detection

[0029] 1. Detection of small molecular substances in serum

[0030] Experimental group: 0.5uL of mixed amino acid sample (concentration: 200nM) was added dropwise on the polished steel target plate MTP384, after air-drying, 1uL of silver nanowire material was added dropwise, the concentration of silver nanowire material was 1mg / ml, and it was completely dried After getting on the machine, mass spectrometry detection was performed on the Autoflex Max mass spectrometer (Bruker Daltonics, Bremen, Germany). During the detection process, the smartbeam-II laser was used to acquire the spectrum in the reflection positive mode of 355nm, and the laser frequency was 1000hz. 20 different points are randomly measured for each sample, and each point is randomly dotted 25 times by laser, so 500 satisfactory dots can be obtained.

[0031] Control group: the silver nanowire material was ...

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Abstract

The invention provides an application of a silver nanowire material as an MALDI-TOF MS matrix in metabolite detection. The analysis method using the nano material as the MALDI-TOF MS matrix is suitable for mass spectrometry analysis of small molecules with molecular weight of less than 1000; the detection difficulty of a small molecule sample is greatly simplified; the detection sensitivity of MALDI-TOF MS of the small molecule sample is improved; and meanwhile, elimination of mass spectrum peak interference generated by an existing matrix and enhancement processing of a mass spectrum signal of the small molecule sample can be realized.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and in particular relates to the application of a silver nanowire material as a MALDI-TOF MS matrix in the detection of metabolites. Background technique [0002] The application of metabolomics to clinical oncology research has become an area of ​​research in recent years as metabolic alterations can directly participate in transformation processes or support biological processes that enable tumor growth, and small molecule metabolites can be a unique source of cancer-specific information. hotspot. During this period, mass spectrometry detection methods are also playing an increasingly important role. [0003] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology has become a useful technique in the analysis of biomolecules such as peptides, proteins and oligosaccharides. However, although there are many types of organic smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64B82Y40/00B82Y30/00
CPCB82Y30/00B82Y40/00G01N27/64
Inventor 王云兵张华胡钦胜钟晟
Owner SICHUAN UNIV
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