Method for artificially inducing gynogenetic flowers on large scale and application of gynogenetic flowers

Abstract

The invention discloses a method for artificially inducing gynogenetic flowers on a large scale, which comprises the following steps: firstly, selecting sexually mature male koi and female flowers for artificial spawning induction, then mixing koi sperms and flower eggs which are subjected to ultraviolet inactivation, and stirring with feather to activate the eggs, the activated ova are placed in yellow mud at the temperature of 0-1 DEG C for cold shock treatment for 20-22 min, then the activated ova are sequentially placed in water at the temperature of 4-6 DEG C, 8-10 DEG C and 15-18 DEG C for treatment for 1-2 min respectively, then the activated ova are placed in an incubation tank at the water temperature of 22-25 DEG C for incubation, and diploid gynogenetic flowers are obtained after identification and screening. According to the method, damage to the fertilized eggs caused by sharp temperature rise can be reduced, the hatching rate of the fertilized eggs and the survival rate of offspring are improved, the technical obstacle that survivable gynogenetic flowers are difficult to obtain through a conventional method is effectively overcome, a gynogenetic flower group is successfully obtained, and the blank in the flower gynogenetic technology aspect is filled up.

Description

technical field [0001] The invention belongs to the technical field of fish breeding, in particular to an artificial large-scale induced gynogenesis flower The method and gynogenesis of flowers Applications. Background technique [0002] Gynogenesis is an important genetic breeding method and an effective way to quickly establish pure lines. 1-2 generations of gynogenesis are equivalent to the continuous selection of 8-10 generations of traditional inbreeding. Through gene purification, individuals carrying harmful genes are naturally eliminated during gynogenesis, so the offspring of gynogenesis often show excellent traits such as hypoxia tolerance, strong stress resistance, and fast growth. [0003] flower , commonly known as hemp carp, hemp fish, big drum eyes, etc. flower The meat is tender, the meat yield is high, the thorns are few, and the protein and polyunsaturated fatty acid content are higher than those of other famous carp fish. It has better health care...

AI Technical Summary

Problems solved by technology

And flowers As a characteristic fish in our country, due to its sensitivity to oxygen content, its low oxygen tolerance is relatively poor in the breeding process, and a large number of deaths often occur due to insufficient oxygen supply during transportation and cultivation. Gynogenesis is difficult. Not yet gynogenetic flowers According to successful reports, there is still a lack of excellent varieties with strong low-oxygen tolerance in the whole country, so the development of innovative breeding techniques is very important for flower The creation of new varieties is of great significance

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