31 results about "Fluid tissues" patented technology

A device positionable in a cavity of a bodily organ (e.g., a heart) may discriminate between fluid (e.g., blood) and non-fluid tissue (e.g., wall of heart) to provide information or a mapping indicative of a position and/or orientation of the device in the cavity. Discrimination may be based on flow, or some other characteristic, for example electrical permittivity or force. The device may selectively ablate portions of the non-fluid tissue based on the information or mapping. The device may detect characteristics (e.g., electrical potentials) indicative of whether ablation was successful. The device may include a plurality of transducers, intravascularly guided in an unexpanded configuration and positioned proximate the non-fluid tissue in an expanded configuration. Expansion mechanism may include helical member(s) or inflatable member(s).

The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multi-use biomolecule lysate. This method allows for simultaneous extraction, isolation, solublization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.

The invention provides methods for diagnosing diseases such as cancer and other conditions using biological samples. Liquid Tissue samples prepared from histopathologically prepared tissue obtained from a subject surprisingly can be used to identify and, optionally, to quantify analytes that are diagnostic of the presence of a disease, condition or syndrome in the subject.

The invention provides methods for multiplex analysis of biological samples of formalin-fixed tissue samples. The invention provides for a method to achieve a multiplexed, multi-staged plurality of Liquid Tissue preparations simultaneously from a single histopathologically processed biological sample, where the protocol for each Liquid Tissue preparation imparts a distinctive set of biochemical effects on biomolecules procured from histopathologically processed biological samples and which when each of the preparations is analyzed can render additive and complementary data about the same histopathologically processed biological sample.

An apparatus adapted to position a functional device tip, such as a sensor, in a body site having both fluid and non-fluid tissue components, in a manner that substantially mitigates the impingement of non-fluid tissue impingement on the device tip. One such apparatus can be used to both remove fluid from a tissue site, such as a site exhibiting tissue swelling, and to determine tissue pressure, using a single catheter. An apparatus can provide a pressure sensor that is adapted to be positioned and used within the tissue site itself, without substantial impingement by non-fluid tissue, and optionally also provides the ability to concurrently remove and/or deliver fluids or components thereof to or from the tissue site.

Method and devices are provided for assessing tissue samples from a plurality of tissue sites in a subject using molecular analysis. In certain aspects, devices of the embodiments allow for the collection of liquid tissue samples and delivery of the samples for mass spectrometry analysis.

Each sensor in one or more pairs of sensors is associated with a particular tissue, for example a first tissue location and a second tissue location respectively. Tissue at a particular tissue location may be solid, for example, muscle tissue, fat tissue, etc., or fluid, for example, blood, fluids associated with edema, etc. The area between the two tissue locations associated with a pair of sensors may comprise solid tissue, fluid tissue, an empty chamber, or combinations thereof. For each pair of sensors in the at least one pair of sensors, a first impedance measurement between the pair of sensors and associated with a first frequency is determined. For each pair of sensors in the at least one pair of sensors, a second impedance measurement between the pair of sensors and associated with a second frequency is determined. A comparison of a ratio of the first impedance measurement at a point in time to the second impedance measurement at a corresponding point in time may be made to determine a volume-related value associated with an area located between the first tissue location and the second tissue location.

A system for use in treating a tissue site, e.g., a site exhibiting or at risk for developing compartment syndrome, the system including a monitor for use in controlling the function and operation of one or more apparatuses providing insertable catheters. The system includes at least one apparatus having a catheter portion that includes both a suitably protected, functional device tip, adapted to be positioned and used within the tissue site, and one or more hollow fiber membranes, e.g., an array of such membranes, adapted to be positioned within the tissue site in order to simultaneously collect fluid therefrom. The catheter portion is adapted to be safely inserted and positioned within the tissue site, in a manner that permits it to function there while substantially minimizing direct impingement of the non-fluid tissue on the functional device tip surface.

The following description provides a biotissue sampling apparatus comprising a housing, a firing button configured on a rear-end of the housing; a tube with an aspirating hole configured to inject medicine in a tissue according to a longitudinal direction of a lower end and extended from a front-end of a housing; and a needle assembly that may move from the tube to a biopsy region by a button thereby cut a solid tissue and store or aspire a fluid tissue.

The invention discloses a fast collecting device of liquid tissue in operations. The fast collecting device of the liquid tissue in the operations comprises a collecting bottle and a collecting bottle cap, wherein the collecting bottle is provided with scales, the collecting bottle cap is matched with an opening of the collecting bottle and a sealing washer is placed inside the collecting bottle cap. A long duct and a short duct penetrating through the bottle cap are placed on the collecting bottle cap. The upper ends of two ducts are respectively connected with a main connecting pipe through a tee ball valve to jointly constitute the fast collecting device of the liquid tissue in the operations. The fast collecting device of the liquid tissue in the operations is simple in structure and convenient to use, is used for collecting liquid tissue of parts such as the skull, the chest, the abdominal cavity of a patient in the operation process or blood recovery, and is fast and safe.

Method and devices are provided for assessing tissue samples from a plurality of tissue sites in a subject using molecular analysis. In certain aspects, devices of the embodiments allow for minimally invasive collection of liquid tissue samples and delivery of the samples for mass spectrometry analysis.

The invention discloses a method for separating deoxynucleotide from salmon seminal fluid, which comprises the following separation steps: S1, preparing fresh or frozen salmon seminal fluid tissue bodies, putting the tissue bodies into a glass homogenizer, adding a quantitative cell lysis buffer solution, homogenizing until no tissue clots exist, then transferring the tissue bodies into a centrifugal tube, adding protease K, uniformly mixing the tissue bodies and the protease K, and carrying out centrifugal separation; and S2, performing water bath in a constant-temperature water bath kettle for a fixed time, and intermittently oscillating the centrifugal tube for a plurality of times to preliminarily obtain mixed liquid. According to the method, the deoxynucleotide component in the salmon semen can be separated through the steps of oscillation, separation and the like by adopting a dissolution and centrifugal separation purification mode and adding quantitative protease K, a TE buffer solution, absolute ethyl alcohol and the like, so that the deoxynucleotide separated by the separation method is relatively high in purity and relatively high in purity. Compared with the traditional separation method, the method is simpler and clearer, is very convenient for personnel to operate, can efficiently and simply complete the separation work of deoxynucleotide, and has higher popularization value.

Peptides from the tyrosine-protein kinase receptor UFO protein (AXL) are provided that are particularly advantageous for quantifying the AXL protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed and include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein digest is prepared from the biological sample and the AXL protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described.

The invention discloses a flow deflector for uniform distribution of a plate-fin heat exchanger. According to the invention, a flow deflector inlet and a flow deflector outlet are formed in a main body of the flow deflector, a deflector is arranged at the flow deflector inlet, and communicated with a liquid storage cavity, the liquid storage cavity is communicated with one side of a liquid distributing cavity through a rectifying plate, and the flow deflector outlet is formed in the other side of the liquid distributing cavity. The flow deflector disclosed by the invention is suitable for the plate-fin heat exchanger, the deflector in the flow deflector can distribute fluid which flows into the heat exchanger, the liquid storage cavity can temporarily store and buffer the fluid, the liquid distributing cavity can uniformly distribute the fluid to the heat exchanger, and the fluid is distributed by depending on the pressure to avoid the occurrence of large pressure in the middle and small pressure on both sides when the fluid enters into the heat exchanger to realize uniform distribution of fluid tissues in the heat exchanger so as to improve the heat exchange efficiency.

The invention provides an electric aspirator used for operating room nursing. Rotating wheels are arranged in a shell, and the rear surface of the shell is fixedly connected with a storage barrels; the interior of each rotating wheel is fixedly provided with a transmission shaft; the inner end of each transmission shaft is provided with an intermediate shaft; the inner end of each intermediate shaft is fixedly provided with a square plate; a chuck is arranged at opposite ends of each transmission shaft and the corresponding intermediate shaft; and an adjusting rod is installed on the outer surface of each chuck in a sliding manner. When liquid tissues in flexible bags gradually increase, the flexible bags push push plates to move towards the inner side so as to extrude bottom ends of the adjusting rods; on the principle of lever, after the top end of the adjusting rod drives the adjacent chuck to be clamped, the intermediate shafts and the transmission shafts rotate together, and accordingly, the rotating wheels are driven to rotate by a partial angle in the shell; the internal solid tissues are swung by sloping hoppers into the storage barrels, so that solid-liquid separation is carried out on aspirates in an operation to obtain tissues in a bottle, thus avoiding the problem of secondary sorting operation.

The invention belongs to the technical field of biology, and particularly relates to a method for extracting DNA with pleuroperitoneal fluid tissue and a kit. The method for extracting DNA with pleuroperitoneal fluid tissue includes the following steps of pleuroperitoneal fluid pretreatment, cell lysis, nucleic acid adsorption, magnetic separation, protein removal and nucleic acid release from magnetic beads. The kit is prepared through the method. By the adoption of the method and the kit, the high-quality DNA can be efficiently and rapidly obtained, the demands, including PCR amplification,quantitative and qualitative analysis, sequencing and others, of molecular biology experiments can be met, application value is realized for diagnosis of related diseases generated by pleuroperitonealfluid, and a new conception is provided for preparation of DNA in pleuroperitoneal fluid tissue.

The present disclosure relates to methods and compositions comprising dissociated buccal mucosa tissue that are useful for treating a wound. Kits for preparing the liquid suspension of dissociated buccal mucosa tissue and treating the wound are also disclosed.