Minimally invasive collection probe and methods for the use thereof

a collection probe and minimally invasive technology, applied in the field of medicine, molecular biology and biochemistry, can solve the problems of difficult recognition of certain tumor cells, unreliable and subjective analysis, and many challenges, and achieve the effect of easy identification, easy reading and removal

Inactive Publication Date: 2020-10-01
BOARD OF RGT THE UNIV OF TEXAS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0027]A further embodiment provides a method of identifying a sampled tissue site and a method to communicate location of the site to the device (probe) operator. Identification of a sampled tissue site allows the operator to access the molecular information recorded at sampled tissue site at a time after sampling molecules collected from the tissue. At least three types of identification approaches are recognized. In the first approach, an exogenous material is attached to the sampled tissue site that identifies the sampled molecular information. In a second approach, the device (probe) is equipped with a tracking sensor/emitter that allows recording the location of the probe (device) and communication to an imaging device when the molecular information is sampled. In a third approach, the tissue region is modified so that the site may be easily identified after harvesting tissue molecules. In the first approach, materials that may be attached to the sampled tissue site include, for example, a suture, a surgical clip, a biocompatible polymer that adheres to the tissue, or an RFID chip that is attached to a magnetic bead that allows easy reading and remo

Problems solved by technology

While intraoperative frozen section analysis has been performed in clinical practice for decades, it presents many challenges.
Freezing artifacts occur during tissue processing and interfere with tissue structure and cell morphology, thus complicating pathologic interpretation, causing the

Method used

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  • Minimally invasive collection probe and methods for the use thereof
  • Minimally invasive collection probe and methods for the use thereof
  • Minimally invasive collection probe and methods for the use thereof

Examples

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example 1

Minimally Invasive Probe for Mass Spectrometry Design

[0102]The system developed consists of three main parts: 1) a syringe pump that is programmed to deliver a discrete solvent volume using a controlled flow rate; 2) tubing systems integrated to two-way pinch valves for controlled solvent and gas transport; 3) a probe tip which is used for direct sampling of biological tissues. The tubing systems and probe tip are also integrated into a minimally invasive surgical device such as a cannula or catheter for use in laparoscopic or endoscopic surgeries. Several iterations of the system were explored and optimized with the ultimate goal of minimizing tissue damage, maximizing tissue-analyte extraction, and maximizing solvent transmission to the mass spectrometer. FIG. 1 shows a schematic figure of one example of a minimally invasive apparatus for analyzing biological tissue. The syringe pump feeds solvent and gas into the minimally invasive probe via micro-PTFE tubing. The probe maintains...

example 2

Molecular Profiles and Analysis

[0105]The system described herein operates by directly connecting the transfer tube to the mass spectrometer inlet for transporting the analyte-containing solvents to the mass spectrometer for molecular analysis. This set up greatly simplifies operational details and precludes the use of ionization sources. After the probe interacts with the tissue, the solvent is then transported to the mass spectrometer and directly infused without the need of an additional ionization source. Since the system is fully automated so that each 10 μL solvent droplet is delivered separately to the inlet, the mass spectrometer operates without any impact on its performance. Rich molecular information is obtained in this manner, similar to what is observed from other solvent-extraction ambient ionization techniques such as desorption electrospray ionization. The ionization mechanism may be similar to inlet ionization. For inlet ionization methods, the ionization occurs in t...

example 3

System Automation for Handheld and Laparoscopic Use

[0116]Because all the materials (PDMS and PTFE) and solvent (only water) used in the minimally invasive probe design are biologically compatible, the system has a high potential to be used in laparoscopic and endoscopic surgeries for real-time analysis. More than that, due to the small dimension of the device, it can be integrated to a robotic surgical system, such as the Da Vinci surgical system, through an accessory port or one of its robotic arms. Several regions of the human body cavity can be quickly sampled during surgery with or without wash / flush steps in between each analysis, and analyzed by using a database of molecular signatures and machine learning algorithms. Therefore, the diagnosing results may be provided in real time for each sampled region. This system can be broadly used in a wide variety of oncological and other surgical interventions (such as endometriosis) for which real-time characterization and diagnosis of...

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Abstract

Method and devices are provided for assessing tissue samples from a plurality of tissue sites in a subject using molecular analysis. In certain aspects, devices of the embodiments allow for minimally invasive collection of liquid tissue samples and delivery of the samples for mass spectrometry analysis.

Description

[0001]This application is a continuation of International Application No. PCT / US2018 / 062625 filed Nov. 27, 2018, which claims the benefit of U.S. Provisional Patent Application No. 62 / 591,179, filed Nov. 27, 2017 and 62 / 640,385, filed Mar. 8, 2018, each of which are incorporated herein by reference in their entirety.[0002]This invention was made with government support under Grant No. R00 CA190783 awarded by the National Institutes of Health. The government has certain rights in the invention.BACKGROUND OF THE INVENTION1. Field of the Invention[0003]The present invention relates generally to the field of medicine, molecular biology and biochemistry. More particularly, it concerns methods and devices for assessment of tissue samples using mass spectrometry.2. Description of Related Art[0004]Clinical diagnosis is commonly performed through the evaluation of tissue samples pre-operatively, intra-operative, and post-operatively, at several other stages of the patient's treatment process...

Claims

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Application Information

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IPC IPC(8): A61B5/00G01N33/483H01J49/04H01J49/16A61B1/313A61B17/34A61B10/02A61M25/00A61B34/30
CPCG01N33/4833A61B2090/395A61B5/0075H01J49/0431A61B10/06A61B1/3132A61B34/30A61M25/0026A61B2090/3983A61B17/34H01J49/165A61B10/02G01N1/4055A61B10/0283A61B17/29A61B10/04H01J49/0404H01J49/0459H01J49/04
Inventor EBERLIN, LIVIA SCHIAVINATOMILNER, THOMASZHANG, JIALINGGIESE, NOAHKATTA, NITESH
Owner BOARD OF RGT THE UNIV OF TEXAS SYST
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