Method for extracting glycosaminoglycan from thelenota ananas

Abstract

The invention belongs to the field of extracting natural active substances by a biological technology and particularly relates to a method for extracting glycosaminoglycan from thelenota ananas. The method comprises the following steps of Step (1) pretreating the thelenota ananas, Step (2) conducting alkaline hydrolysis on a body wall of the thelenota ananas, Step (3) conducting double-enzymolysis on the body wall of the thelenota ananas, Step (4) using trichloroacetic acid to remove protein in a double-enzymolysis solution after finishing the enzymolysis, Step (5) using ethanol to precipitate glycosaminoglycan of the thelenota ananas, Step (6) drying raw glycosaminoglycan of the thelenota ananas, Step (7) using hydrogen peroxide to decolorize, Step (8) using a cellulose ion-exchange column chromatographic method to purify glycosaminoglycan of the thelenota ananas, and Step (9) drying refined glycosaminoglycan of the thelenota ananas. The method can balance a relation between yield and purity of glycosaminoglycan of the thelenota ananas, and improves the purity of glycosaminoglycan of the thelenota ananas.

Description

technical field [0001] The invention belongs to the field of extracting natural active substances in biotechnology, and in particular relates to a method for extracting glycosaminoglycan from plum blossom ginseng. Background technique [0002] Chronic hepatitis B is a serious health problem facing the world. China, Southeast Asia and Africa are areas with high incidence of HBV infection. At the same time, the incidence of HBV-related liver diseases such as primary liver cancer in these areas is also significantly higher than that in central and southern America. Low incidence area of ​​HBV infection. The treatment of chronic hepatitis B mainly includes anti-virus, immune regulation, anti-inflammatory and liver protection, anti-fibrosis and symptomatic treatment. Among them, effective anti-viral treatment and lasting suppression of HBV DNA below the routine detection range of PCR are the key to treatment. Sulfated polysaccharides have shown different degrees of antiviral, an...

AI Technical Summary

Problems solved by technology

However, the above-mentioned preparation method cannot obtain high-purity glycosaminoglycans of plum blossom ginseng. The main reason is that the chemical structures of glycosaminoglycans have significant overlap, such as molecular weight difference method, inconsistent sulfation degree, complex conception, and inconsistent microstructure. Uniformity and changes in the number of residues, etc., especially on a specific protein glycan molecule, there are differences in the type and number of glycosaminoglycan chains contained in it, which makes it difficult to obtain the purity High Prunus Glycosaminoglycans