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Analysis method for determining oligomerization thelenota ananas glycosaminoglycan content

A technology of ginseng glycosamine and pineapple, applied in the analysis field of determining the content of oligomeric pineapple ginseng glycosaminoglycans

Active Publication Date: 2014-06-18
SHENZHEN NEPTUNUS PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In the prior art, there is no report on the analysis and determination of oligomeric pineapple glycosaminoglycans by using high performance liquid chromatography. This paper establishes an analysis method for this product for the first time

Method used

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  • Analysis method for determining oligomerization thelenota ananas glycosaminoglycan content
  • Analysis method for determining oligomerization thelenota ananas glycosaminoglycan content
  • Analysis method for determining oligomerization thelenota ananas glycosaminoglycan content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] [embodiment 1] the selection of chromatographic column

[0029] Chromatographic conditions and system suitability: Gel chromatographic columns, Shodex Ohpak SB-804HQ, Viscotek, TOSOHTSK-gel, Agilent PL Aquagel-OH and other four gel chromatographic columns were selected respectively. Use 0.1mol / L sodium chloride as the mobile phase; the flow rate is 0.5ml / min; the column temperature is 35°C; the temperature of the differential detector is 35°C. The number of theoretical plates should not be less than 1000 based on the calculation of the D2 peak of dextran.

[0030] Determination method: Freeze-dried fengshensu preparation for injection, batch number: 20071201, take the contents, accurately weigh an appropriate amount (about 100 mg of fengshensu), put it into a 10ml measuring bottle, add 0.1mol / L sodium chloride solution Dissolve, dilute to the mark, shake well, filter, take the subsequent filtrate as the test solution; take another appropriate amount of fengshensu refer...

Embodiment 2

[0032] [embodiment 2] the selection of flow rate

[0033] Chromatographic conditions and system suitability: Shodex Ohpak SB-804HQ gel chromatography column; 0.10mol / L sodium chloride as the mobile phase; flow rates are 0.2ml / min, 0.3ml / min, 0.5ml / min, 0.7ml / min, 0.8ml / min, the column temperature is 35°C; the differential detector temperature is 35°C. The number of theoretical plates should not be less than 1000 based on the calculation of the D2 peak of dextran.

[0034] Determination method: Freeze-dried fengshensu preparation for injection, batch number: 20071201, take the contents, accurately weigh an appropriate amount (about 100 mg of fengshensu), put it into a 10ml measuring bottle, add 0.1mol / L sodium chloride solution Dissolve, dilute to the mark, shake well, filter, take the subsequent filtrate as the test solution; take another appropriate amount of fengshensu reference substance that has been dried under reduced pressure with phosphorus pentoxide to constant weig...

Embodiment 3

[0036] [Example 3] mobile phase ratio selection

[0037] Chromatographic conditions and system suitability: Shodex Ohpak SB-804HQ gel chromatography column; 0.07mol / L, 0.08mol / L, 0.10mol / L, 0.12mol / L, 0.13mol / L sodium chloride as the mobile phase; The flow rate is 0.5ml / min; the column temperature is 35°C; the differential detector temperature is 35°C. The number of theoretical plates should not be less than 1000 based on the calculation of the D2 peak of dextran.

[0038] Determination method: Freeze-dried fengshensu preparation for injection, batch number: 20071201, take the contents, accurately weigh an appropriate amount (about 100 mg of fengshensu), put it into a 10ml measuring bottle, add 0.1mol / L sodium chloride solution Dissolve, dilute to the mark, shake well, filter, take the subsequent filtrate as the test solution; take another appropriate amount of fengshensu reference substance that has been dried under reduced pressure with phosphorus pentoxide to constant weig...

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Abstract

The present invention discloses a method for determining oligomerization thelenota ananas glycosaminoglycan content. The method is characterized by comprising: (1) respectively preparing an oligomerization thelenota ananas glycosaminoglycan sample solution and a fengshensu control sample solution; (2) adopting high performance liquid chromatography to analyze the oligomerization thelenota ananas glycosaminoglycan sample solution, wherein the chromatography conditions comprise that: the Shodex OhpakSB-804HQ type gel chromatography column is adopted, the mobile phase is 0.08-0.12 mol / L NaCl, the column temperature is 34-36 DEG C, the flow rate is 0.3-0.7 ml / min, the detection wavelength is 206+ / -2 nm, and the injection volume is 20-30 mul; and (3) calculating the oligomerization thelenota ananas glycosaminoglycan content according to the high performance liquid chromatography analysis result.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an analysis method for measuring the content of oligomeric pineapple ginseng glycosaminoglycans. The present invention is based on the application number 200910110114.0 submitted by the applicant on November 6, 2009, titled "oligo-fucosylated glycosaminoglycan and its preparation method" and the application number 200910109861.2 submitted on November 25, 2009 , a further invention of the invention patent titled "Oligomeric Pineapple Glycosaminoglycan and Its Preparation Method", the full text of the patent application is incorporated in the description of the present invention in the form of references. Background technique [0002] Oligomeric pineapple glycosaminoglycan refers to a kind of glycosaminoglycan derived from the body wall or viscera of echinoderms, which has a main chain structure similar to chondroitin sulfate, but has side chain fucose sulfate substitu...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 严启新于琳梁慧曾伟珍康晖王兵冯汉林
Owner SHENZHEN NEPTUNUS PHARM CO LTD
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