Method for extracting and purifying glycosaminoglycans (GAGs) from thelenota ananas

A technology of glycosaminoglycans and glycosamines, which is applied in the field of extraction of natural active substances in biotechnology, can solve problems such as complex conception, changes in the number of residues, and molecular weight differences, and achieve the effect of improving purity

Inactive Publication Date: 2013-03-27
QINGDAO MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned preparation method cannot obtain high-purity glycosaminoglycans of plum blossom ginseng. The main reason is that the chemical structures of glycosaminoglycans have significant overlap, such as molecular weight difference method, inconsistent sulfation degree, complex concepti...

Method used

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Effect test

Embodiment 1

[0013] The method for extracting glycosaminoglycans from plum blossom ginseng in this embodiment comprises the following steps: Step 1: Pretreatment of plum blossom ginseng; Step 2: Alkaline hydrolysis of plum blossom ginseng body wall; Step 3: Double enzyme enzymolysis of plum blossom ginseng body wall; Step 4: After the enzymatic hydrolysis, use trichloroacetic acid to remove the protein in the double-enzyme hydrolyzate; step 5: use ethanol to precipitate the glycosaminoglycan of plum flower ginseng; step 6: dry the glycosaminoglycan of plum flower ginseng; step 7: hydrogen peroxide Decolorization; step 8: purifying the glycosaminoglycans of plum blossom ginseng by cellulose ion exchange column chromatography; step 9: precipitating the glycosaminoglycans of plum blossom ginseng with potassium acetate; step 10: drying the glycosaminoglycans of plum blossom ginseng.

[0014] The specific method of each step is:

[0015] Step 1: Pretreatment of plum blossom ginseng; quickly rin...

Embodiment 2

[0029] The method for extracting glycosaminoglycans from plum blossom ginseng in this embodiment comprises the following steps: Step 1: Pretreatment of plum blossom ginseng; Step 2: Alkaline hydrolysis of plum blossom ginseng body wall; Step 3: Double enzyme enzymatic hydrolysis of plum blossom ginseng body wall; Step Four: After the enzymatic hydrolysis, use trichloroacetic acid to remove the protein in the double-enzyme hydrolyzate; step five: use ethanol to precipitate the glycosaminoglycan of plum flower ginseng; step six: dry the glycosaminoglycan of plum flower ginseng; step seven: hydrogen peroxide Decolorization; step 8: purifying the glycosaminoglycans of plum blossom ginseng by cellulose ion exchange column chromatography; step 9: precipitating the glycosaminoglycans of plum blossom ginseng with potassium acetate; step 10: drying the glycosaminoglycans of plum blossom ginseng. The specific method of each step is:

[0030] Step 1: Pretreatment of plum blossom ginseng;...

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Abstract

The invention belongs to the field of extraction of biotechnology natural active materials and particularly relates to a method for extracting and purifying GAGs from thelenota ananas. The method includes: step one, pre-processing thelenota ananas; step two, performing alkaline hydrolysis on the body wall of the thelenota ananas; step three, performing two-enzyme enzymolysis on the body wall of the thelenota ananas; step four, removing proteins in two-enzyme enzymatic hydrolysate through trichloroacetic acid after the enzymolysis; step five, precipitating the GAGs of the thelenota ananas through ethanol; step six, drying GAGs raw sugar of the thelenota ananas; step seven, purifying the GAGs of the thelenota ananas by using a cellulose ion exchange column chromatography and a gel column sequentially; and step eight, drying GAGs refined sugar of the thelenota ananas. According to the method, the relation between the yield and the purity of the GAGs of the thelenota ananas can be balanced, and the purity of the GAGs of the thelenota ananas can be improved.

Description

technical field [0001] The invention belongs to the field of extracting natural active substances in biotechnology, and in particular relates to a method for extracting and purifying glycosaminoglycans from plum blossom ginseng. Background technique [0002] Chronic hepatitis B is a serious health problem facing the world. China, Southeast Asia and Africa are areas with high incidence of HBV infection. At the same time, the incidence of HBV-related liver diseases such as primary liver cancer in these areas is also significantly higher than that in central and southern America. Low incidence area of ​​HBV infection. The treatment of chronic hepatitis B mainly includes anti-virus, immune regulation, anti-inflammatory and liver protection, anti-fibrosis and symptomatic treatment. Among them, effective anti-viral treatment and lasting suppression of HBV DNA below the routine detection range of PCR are the key to treatment. Sulfated polysaccharides have shown different degrees o...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 宣世英李雪洁林中华姜曼姜相君辛永宁
Owner QINGDAO MUNICIPAL HOSPITAL
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