Method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid

A technology of antibacterial polypeptides and working fluids, which is applied in the field of extracting antibacterial polypeptides by using the working fluid of imitated thorns
CN105368901AInactive Publication Date: 2016-03-02SHANDONG HOMEY AQUATIC DEV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG HOMEY AQUATIC DEV
Publication Date
2016-03-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid. The method comprises the steps of taking the apostichopus japonicus working fluid as a raw material and obtaining concentrated solution through electroosmosis desalting and vacuum drying; performing enzymolysis of the concentrated solution, performing centrifugation to take enzymolysis supernatant, and performing vacuum concentration and freeze drying to obtain solid powder; re-dissolving the solid powder in deionized water, performing ethanol precipitation of polysaccharide, taking precipitated dissolved liquid, adopting a gel filtration chromatography method to collect absorption peak eluent, causing the absorption peak eluent to undergo bacteriostatic activity detection, merging the absorption peak eluent which has bacteriostatic activity according to the detection, performing freeze drying, and obtaining composite antibacterial polypeptide. The method for extracting the antibacterial polypeptide is rational in process and feasible in operation, the antibacterial polypeptide extracted according to the method is reliable in source and high in safety, and the later-period research is convenient.
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Description

technical field

[0001] The invention relates to the preparation of polypeptides, in particular to a method for extracting antibacterial polypeptides by using the thorn-like working liquid. Background technique

[0002] We know that biologically active peptides are a general term for different peptides from dipeptides to complex linear and circular structures composed of 20-60 natural amino acids in different compositions and arrangements, and are multifunctional compounds derived from proteins. . Active peptides have a variety of human metabolism and physiological regulation functions, are easy to digest and absorb, have the functions of promoting immunity, hormone regulation, antibacterial, antiviral, lowering blood pressure, lowering blood fat, etc., and are extremely safe to eat. They are ideal for drug screening, vaccine preparation and food preparation. The treasure house of natural resources of additives is also the most popular research topic in the current internati...

Claims

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