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Method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid

A technology of antibacterial polypeptides and working fluids, which is applied in the field of extracting antibacterial polypeptides by using the working fluid of imitated thorns

Inactive Publication Date: 2016-03-02
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the extraction of antibacterial polypeptides by using the artificial japonicus working solution

Method used

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  • Method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid
  • Method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid
  • Method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid

Examples

Experimental program
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Embodiment 1

[0039] A method for extracting antibacterial polypeptides by using a thorn-like working liquid, which undergoes the following process steps:

[0040] (1) Raw material selection and processing

[0041] Select fresh imitation sea cucumber, dissect and remove the intestines, put it in boiling water and cook for 20 minutes, remove the body and obtain the imitation sea cucumber working solution as raw material, cool it rapidly to 6°C, control the speed of the tube-type machine at 6000r / min, centrifuge 20min, remove the insoluble matter in the working liquid of imitation thorn to obtain the supernatant;

[0042] (2) Desalination

[0043] The supernatant obtained in step (1) was desalted by dialysis with an electric drive device, the voltage was controlled at 24V, and the flow rate was controlled at 30L / h to obtain a desalted solution;

[0044] (3) Vacuum concentration

[0045] Concentrate the desalted solution obtained in step (2) to 1 / 15 of the volume of the original solution un...

Embodiment 2

[0064] A method for extracting antibacterial polypeptides by using a thorn-like working liquid, which undergoes the following process steps:

[0065] (1) Raw material selection and processing

[0066] Select fresh imitation sea cucumber, dissect and remove the intestines, put it in boiling water and cook for 30 minutes, take out the body and obtain the imitation sea cucumber working solution as raw material, cool it rapidly to 4°C, and centrifuge at a controlled speed of 4000r / min for 30 minutes in a tubular centrifuge , remove the insoluble matter in the working liquid of imitation thorn to obtain the supernatant;

[0067] (2) Desalination

[0068] The supernatant obtained in step (1) is desalted by dialysis with an electric drive device, the voltage is controlled to 30V, and the flow rate is controlled to 70L / h to obtain a desalted solution;

[0069] (3) Vacuum concentration

[0070] Concentrate the desalted solution obtained in step (2) to 1 / 20 of the volume of the origi...

Embodiment 3

[0085] A method for extracting antibacterial polypeptides by using a thorn-like working liquid, which undergoes the following process steps:

[0086] (1) Raw material selection and processing

[0087] Select fresh imitation sea cucumber, dissect and remove the intestines, put it in boiling water and cook for 10 minutes, take out the body and obtain the imitation sea cucumber working solution as raw material, cool it rapidly to 10°C, and centrifuge at a controlled speed of 4000r / min for 20 minutes in a tubular centrifuge ,, remove the insoluble matter in the working liquid of imitation thorn to obtain the supernatant;

[0088] (2) Desalination

[0089] The supernatant obtained in step (1) was desalted by dialysis with an electric drive device, the voltage was controlled to 16V, and the flow rate was controlled to 10L / h to obtain a desalted solution;

[0090] (3) Vacuum concentration

[0091] Concentrate the desalted solution obtained in step (2) to 1 / 10 of the volume of the ...

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Abstract

The invention discloses a method for extracting antibacterial polypeptide by utilizing apostichopus japonicus working fluid. The method comprises the steps of taking the apostichopus japonicus working fluid as a raw material and obtaining concentrated solution through electroosmosis desalting and vacuum drying; performing enzymolysis of the concentrated solution, performing centrifugation to take enzymolysis supernatant, and performing vacuum concentration and freeze drying to obtain solid powder; re-dissolving the solid powder in deionized water, performing ethanol precipitation of polysaccharide, taking precipitated dissolved liquid, adopting a gel filtration chromatography method to collect absorption peak eluent, causing the absorption peak eluent to undergo bacteriostatic activity detection, merging the absorption peak eluent which has bacteriostatic activity according to the detection, performing freeze drying, and obtaining composite antibacterial polypeptide. The method for extracting the antibacterial polypeptide is rational in process and feasible in operation, the antibacterial polypeptide extracted according to the method is reliable in source and high in safety, and the later-period research is convenient.

Description

technical field [0001] The invention relates to the preparation of polypeptides, in particular to a method for extracting antibacterial polypeptides by using the thorn-like working liquid. Background technique [0002] We know that biologically active peptides are a general term for different peptides from dipeptides to complex linear and circular structures composed of 20-60 natural amino acids in different compositions and arrangements, and are multifunctional compounds derived from proteins. . Active peptides have a variety of human metabolism and physiological regulation functions, are easy to digest and absorb, have the functions of promoting immunity, hormone regulation, antibacterial, antiviral, lowering blood pressure, lowering blood fat, etc., and are extremely safe to eat. They are ideal for drug screening, vaccine preparation and food preparation. The treasure house of natural resources of additives is also the most popular research topic in the current internati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 贾爱荣刘昌衡孙永军刘新袁文鹏张绵松夏雪奎鞠文明唐晓波乔若瑾胡炜史亚萍赵博张永刚
Owner SHANDONG HOMEY AQUATIC DEV
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