Marine bacterium capable of antagonizing pathogenic vibrio splendidus and application of marine bacteria

Abstract

The invention discloses a marine bacterium capable of antagonizing pathogenic vibrio splendidus and an application of the marine bacterium. The marine bacterium is characterized in that the marine bacterium is a marine-derived vibrio 33 preserved in a common microorganism center of a China committee for culture collection of microorganisms on May 30, 2016, and the preservation number is CGMCC No. 12561. The vibrio 33 is derived from a healthy marine organism, screened by a filtering paper method and authenticated as a vibrio by 16S rRNA (ribosomal ribonucleic acid). The vibrio 33 obviously functions in inhibiting the growth and infection process of important pathogenic vibrio splendidus of 'skin ulcer syndrome' of apostichopus japonicus. The marine bacterium has the advantages that equivalent vibrio splendidus and vibrio 33 are added into seawater, the quantity of the vibrio splendidus can be reduced by 86.7%, the relative survival rate of the apostichopus japonicus can be increased by 50% as the vibrio 33 is added in the process of infecting the apostichopus japonicus by the vibrio splendidus, and the marine bacterium is expected to play an important role in control of the pathogenic vibrio splendidus and apostichopus japonicus diseases caused by the pathogenic vibrio splendidus.

Description

technical field [0001] The invention relates to a marine bacterium, in particular to a marine bacterium capable of antagonizing the pathogenic bacteria Vibrio splendidus and its application. Background technique [0002] Vibrio splendidus is a common and important opportunistic pathogen in mariculture environments. When environmental conditions are unfavorable, it can infect American redfish (Helen et al. 2009), turbot (Gatesoupe 1999; Thomson 2005), shellfish (Saulnier 2010; Rosa et al. 2012; Liu et al. 2013) and japonicus (Wang Yingeng 2006; Wang Yingeng 2013; Li Qiang 2013) and other mariculture animals, causing frequent and large-scale occurrence of aquatic animal diseases. The output value of a single species of imitation japonicus is the highest, and the output value increases at a rate of 20% every year. It has become one of the important pillars of my country's aquaculture industry. However, in recent years, with the continuous expansion of sea cucumber breeding sca...

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Problems solved by technology

[0003] At present, the commonly used methods to control epidemic diseases such as "rotting skin syndrome" of sea cucumbers are generally to use chemical drugs, including long-term large-scale and large-scale application of antibiotics or chemical preparations. Although these drugs can temporarily kill or inhibit pathogenic bacteria effectively, but It will also cause problems such as the residue of antibiotics and other chemical agents in the environment, the spread of drug-resistant genes, and the emergence of drug-resistant bacteria, and cannot fundamentally control the occurrence of diseases

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