A kind of marine bacterium capable of antagonizing pathogenic bacteria Vibrio splendidus and its application

Abstract

The invention discloses a marine bacterium capable of antagonizing the pathogenic Vibrio brilliant and its use, characterized in that the marine bacterium is Vibrio 33 from the ocean, and was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 30, 2016 , the preservation number is CGMCC No.12561; Vibrio 33 is derived from healthy marine organisms, which was screened by the filter paper method and identified as Vibrio by 16S rRNA; The growth and infection process of Vibrio splendidus, an important pathogen of skin syndrome, can be significantly inhibited. The advantage is that the number of Vibrio splendidus can be reduced by 86.7% when the same amount of Vibrio splendidus and Vibrio 33 are added to seawater; Adding Vibrio 33 to A. japonicus in the process of infecting A. japonicus can increase the relative survival rate of A. japonicus by 50%. This bacterium is expected to play an important role in controlling the pathogenic Vibrio resp.

Description

technical field [0001] The invention relates to a marine bacterium, in particular to a marine bacterium capable of antagonizing the pathogenic bacteria Vibrio splendidus and its application. Background technique [0002] Vibrio splendidus is a common and important opportunistic pathogen in mariculture environments. When environmental conditions are unfavorable, it can infect American redfish (Helen et al. 2009), turbot (Gatesoupe 1999; Thomson 2005), shellfish (Saulnier 2010; Rosa et al. 2012; Liu et al. 2013) and japonicus (Wang Yingeng 2006; Wang Yingeng 2013; Li Qiang 2013) and other mariculture animals, causing frequent and large-scale occurrence of aquatic animal diseases. The output value of a single species of imitation japonicus is the highest, and the output value increases at a rate of 20% every year. It has become one of the important pillars of my country's aquaculture industry. However, in recent years, with the continuous expansion of sea cucumber breeding sca...

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Problems solved by technology

[0003] At present, the commonly used methods to control epidemic diseases such as "rotting skin syndrome" of sea cucumbers are generally to use chemical drugs, including long-term large-scale and large-scale application of antibiotics or chemical preparations. Although these drugs can temporarily kill or inhibit pathogenic bacteria effectively, but It will also cause problems such as the residue of antibiotics and other chemical agents in the environment, the spread of drug-resistant genes, and the emergence of drug-resistant bacteria, and cannot fundamentally control the occurrence of diseases

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