Screening of Bacillus amyloliquefaciens and application thereof in Apostichopus japonicas culture

A technology of amylolytic spores and bacillus, which is applied in the fields of application, bacteria, and fish farming, and can solve problems such as the deterioration of the breeding environment, residues in animals, and drug-resistant strains

Active Publication Date: 2016-06-08
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the deterioration of the breeding environment and the occurrence of diseases caused by high-density farming and irregular operations have brought huge economic losses to the sea cucumber farming industry.
The traditional method of disease prevention and treatment is mainly to use antibiotics. The use of antibiotics is easy to produce drug-resistant strains, and there are residual problems in animals, which endangers food safety. Therefore, it is necessary to find alternatives to antibiotics.

Method used

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  • Screening of Bacillus amyloliquefaciens and application thereof in Apostichopus japonicas culture
  • Screening of Bacillus amyloliquefaciens and application thereof in Apostichopus japonicas culture
  • Screening of Bacillus amyloliquefaciens and application thereof in Apostichopus japonicas culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Isolation, screening, identification and safety verification of strains

[0013] 1. Sample

[0014] Intestines of healthy sea cucumbers collected from Laizhou sea cucumber farm in Yantai City.

[0015] 2. Culture medium:

[0016] 1) Bacillus isolation medium: glucose 10g, calcium phosphate 5g, ammonium sulfate 0.5g, potassium chloride 0.2g, magnesium sulfate heptahydrate 0.1g, manganese sulfate 0.0001g, ferrous sulfate 0.0001g, yeast extract 0.5g, Distilled water 1000ml, pH6.8-7.2, agar 20g, sterilized at 121°C for 30min.

[0017] 2) Improved TSB medium: tryptone soybean broth medium (TSB) 30g, sodium chloride 10g, distilled water 1000ml, pH7.4-7.6, prepare solid medium and add 15-20g of agar, sterilized at 121°C 20min.

[0018] 3) Starch hydrolysis medium: beef extract 5g, peptone 10g, sodium chloride 5g, soluble starch 2g, distilled water 1000ml, pH7.2-7.4, prepare solid medium, add 15-20g agar, sterilize at 115°C for 20min.

[0019] 4) Casein hydrolysis double-l...

Embodiment 2

[0035] Effects of Bacillus amyloliquefaciens ZYTC1-1 on the growth, immunity and disease resistance of sea cucumber juveniles

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Abstract

The invention relates to the technical field of screening and application of functional microorganisms in particular to screening of Bacillus amyloliquefaciens and application thereof in Apostichopus japonicas culture. The Bacillus amyloliquefaciens is collected on 24th December, 2015 in China Center for Type Culture Collection, abbreviated as CCTCC with the address: Wuchang Luojiashan Wuhan University Collection Center, Wuhan, Hubei, is claimed by Ludong University under CCTCC No. M2015774 and is named as Bacillus amyloliquefaciens ZYTC1-1. The Bacillus amyloliquefaciens is screened from healthy intestines of Apostichopus japonicas, has great ability to produce protease and amylase, can improve the activity of amylase in the testiness of Apostichopus japonicas, promote growth of Apostichopus japonicas and improve immunity and disease resistance of Apostichopus japonicas, and is widely applicable to the field of Apostichopus japonicas culture and aquatic feeds.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening and application, in particular to the screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber cultivation. Background technique [0002] With the increasing demand of sea cucumber at home and abroad and the international development of aquatic product trade, the sea cucumber breeding industry has developed by leaps and bounds in recent years. However, the deterioration of the breeding environment and the occurrence of diseases caused by high-density breeding and irregular operations have brought huge economic losses to the sea cucumber farming industry. The traditional way to prevent and control diseases is mainly to use antibiotics. The use of antibiotics is easy to produce drug-resistant strains, and there are residues in animals, which endangers food safety. Therefore, it is necessary to find alternatives to antibiotics. [0003] As an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01K61/00A23K50/80A23K10/18C12R1/07
CPCA01K61/00C12N1/205C12R2001/07Y02A40/81Y02A40/818
Inventor 赵彦翠袁磊孙虎山万军利王旭静王敏陈昌
Owner LUDONG UNIVERSITY
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