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Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics

A technology of compound microecology and growth rate, which is applied in the field of feed additives and compound microecological preparations, can solve the problems of unfavorable growth and reproduction of sea cucumbers, low feed conversion rate, long growth cycle, etc., achieve fast reproduction speed, improve digestibility, The effect of high germination rate at low temperature

Active Publication Date: 2013-11-27
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The food composition of sea cucumber determines that alginate and cellulose account for a large proportion of its feed, but the ability of sea cucumber to secrete alginate and cellulase is limited, the feed conversion rate is low, and the growth cycle is long
Unfavorable to the growth and reproduction of sea cucumbers

Method used

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  • Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics
  • Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The composite microbial preparation for improving the growth rate of sea cucumber according to the present invention includes the following components: the bacterial content of Bacillus subtilis is not less than 1×10 8 Saccharomyces cerevisiae / g, the bacterial content of Saccharomyces cerevisiae is not less than 1×10 9 pcs / g, cellulase content not less than 1000U / g, pectinase content not less than 1200U / g, xylanase content not less than 1500U / g, neutral protease content not less than 12000U / g, solid The bacterial content of fermented lactic acid bacteria is not less than 200mg / g, and the carrier content is not less than 500mg / g.

[0020] The compound microecological preparation for improving the growth rate of sea cucumber described in this example includes: Bacillus subtilis 1×10 8 pcs / g, Saccharomyces cerevisiae 1×10 9 pcs / g, cellulase 1000U / g, pectinase 1200U / g, xylanase 1500U / g, neutral protease 12000U / g, solid fermented lactic acid bacteria 200mg / g, carrier 500mg...

Embodiment 2

[0022] Example 2. Experiment on the effect of the composite microecological preparation of the present invention on the growth of Apostichopus japonicus

[0023] The volume of the experimental aquarium was 45L, and the air was continuously inflated during the breeding period. The test was divided into 4 groups, the first group was added with 5g / kg enzyme preparation, and the second group was added with 10 9 CFU / kg, the third group added enzyme preparation 5g / kg + bacterial preparation 10 9 CFU / kg, the fourth group was the control group, no enzyme preparation was added. Each group had 6 repetitions, one aquarium was one repetition, and 8 sea cucumbers were stocked in each aquarium, with a size of 9.8g±0.2 / head, and the test period was 40d.

[0024] The enzyme preparations added in the first group are the components of the composite microbial preparations to remove bacterial agents, that is, they are composed of cellulase, pectinase, xylanase and neutral protease with the stat...

Embodiment 3

[0027] Example 3, the effect of the composite microecological preparation of the present invention on the growth of young ginseng

[0028] 2 Apostichopus japonicus seedling workshops were selected for the experiment of adding enzymes. The volume of each seedling pond is 20 cubic meters of water, and the water temperature of the seedling pond is between 11-13 °C. The compound probiotic preparation was added in an amount of 5 g / kg feed, and a blank control group was set. The test period is divided into 20d and 25d. In the 20d group, only the 4-mesh specification was selected as the test object. . Six replicates were set for each experiment, and the weight of each pool of young ginseng was weighed at the beginning and end of the experiment.

[0029] The experimental results are as figure 1 shown. No matter it is 4-mesh seedlings or 6-mesh seedlings, the average weight gain rate of larvae at 20-25 d was significantly increased after continuously adding 5g / kg of compound prob...

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Abstract

The invention provides a composite microecologics capable of improving growth rate of stichopus japonicus and application of the composite microecologics. The composite microecologics comprises bacillus subtilis, saccharomyces cerevisiae, cellulase, pectinase, xylanase, neutral protease, solid fermentation lactic acid bacteria and a carrier, wherein the bacterium content of the bacillus subtilis is greater than or equal to 1* 108 per g, the bacterium content of the saccharomyces cerevisiae is greater than or equal to 1* 109 per g, the content of the cellulase is greater than or equal to 1,000 U / g, the content of the pectinase is greater than or equal to 1,200 U / g, the content of the xylanase is greater than or equal to 1,500 U / g, the content of the neutral protease is greater than or equal to 12,000 U / g, the bacterium content of the solid fermentation lactic acid bacteria is greater than or equal to 200 mg / g, and the content of the carrier is greater than or equal to 500 mg / g. The composite microecologics can effectively improve the utilization efficiency of bait by stichopus japonicus and promote the growth of stichopus japonicus through adding zymin; the added fungicide can restrain reproduction of pathogenic bacteria in a water body and reduce the occurrence probability of diseases such as skin melting, head shaking, swollen mouth and viscus discharging; the composite microecologics has the advantages of safety, efficiency, specifity, no residue, no side effect and the like.

Description

technical field [0001] The invention belongs to the technical field of livestock feed and relates to a feed additive, in particular to a compound microecological preparation for improving the growth rate of sea cucumber and its application. Background technique [0002] At present, in sea cucumber cultivation, most of them use algae powder, scallop powder, etc. with a certain proportion of sea mud as the feed for sea cucumbers, and the proportion of sea mud is between 20% and 50%. In addition to sea mud, algae flour and other plant-based raw materials are dominant in feed materials, so they contain more non-starch polysaccharides, which have certain anti-nutritional effects on animal digestion and absorption. The alginase activity of sea cucumbers from larvae to adults has been at a low level, indicating that sea cucumbers have a weak ability to digest algae rich in alginic acid, such as kelp and wakame. The food composition of japonicus japonicus determines that alginate a...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/00
Inventor 梁晶晶张大伟田相利周莉芬蔡玉勇
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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