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Bacillus amyloliquefaciens and application thereof

A technology of amyloliquefaciens and bacillus, applied in the field of microorganisms, can solve the problems of imitating japonicus bacterial diseases without probiotic preparations, colonization and ineffective effects, etc., achieve outstanding control effects, improve population numbers, transportation and storage convenient effect

Inactive Publication Date: 2017-12-29
FISHERIES RES INST OF FUJIAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are some beneficial microorganism products for aquatic animals on the market, but most of the beneficial microorganisms used in these products come from the environment or other higher animals, and their colonization and effect in the intestinal tract of sea cucumbers are not significant
At present, there are more and more reports on bacterial diseases of japonicus japonicus cultured in southern Fujian, but there are no related reports on the use of probiotics to prevent and control bacterial diseases of japonicus japonicus

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
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Embodiment 1

[0024] (1) Isolation and antagonistic analysis of strains:

[0025] 1) Isolation and purification of bacterial strains:

[0026] Wash the body surface of the experimental sea cucumber with 75% alcohol first. Under sterile conditions, the body cavity of the sea cucumber was cut open with scissors, the digestive tract of the sea cucumber was taken out, the outer wall of the intestine was washed with 0.9% normal saline, and the contents of the intestine were squeezed out. The intestinal contents and intestinal walls of the collected farmed and wild sea cucumbers were placed in sterile test tubes and weighed. Place them in a mortar and grind them thoroughly with 5 mL of sterile saline to obtain a homogeneous sample. Samples were diluted to 10 times with sterile saline -6 , samples of each dilution were shaken evenly with a vortex shaker. Select an appropriate concentration gradient, take 100 μL to coat the 2216E plate, set 3 replicates at each concentration, and culture at 28°...

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Abstract

The invention discloses bacillus amyloliquefaciens and an application thereof, and particularly relates to bacillus CQN-2 which is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.12420. The bacillus amyloliquefaciens disclosed by the invention is separated from healthy apostichopus japonicas intestinal tract fed in the south, strongly antagonizes bacillus of vibrio vulnificus, has an obvious antagonistic effect on pathogenic bacteria-vibrio vulnificus, and can replace an antibiotic drug for treating vibrio vulnificus.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of bacillus amyloliquefaciens and its application. Background technique [0002] Since ancient times, sea cucumber has long been famous as a delicacy on the tables of people in our country, Southeast Asia and other countries along the Pacific Ocean and the West Indian Ocean. Especially Chinese people in the world have the habit of eating sea cucumber as a "food tonic". People call sea cucumber "eight treasures of seafood". "one. Among the economic species of sea cucumbers, Apostichopus japonicus (Selenka) produced in the northern coast has the best quality and is the crown of sea cucumbers. Sea cucumber imitation has the functions of anti-tumor, improving biological immunity, anti-radiation and anti-fatigue, anti-inflammation, reducing blood viscosity, regulating blood lipid, inhibiting virus, and promoting the recovery of hematopoietic function. It i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P31/04C12R1/07
CPCA61K2035/115C12N1/205C12R2001/07
Inventor 黄瑞芳周宸杨求华陆振林琪黄玉琪何丽斌
Owner FISHERIES RES INST OF FUJIAN
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