A kind of preparation method and application of ethanol extract of Cryptocodella sp.

A technology of ethanol extraction and cryptocole, which is applied in the field of marine biology, can solve the problems such as the absence of cryptocole, achieve the effects of less consumption of organic solvent, simple process and prolonging the average lifespan

Active Publication Date: 2021-03-02
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no report on the related application of Cryptocodida in the prior art, especially the report on the anti-oxidation application of the ethanol extract of Cryptocodida

Method used

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  • A kind of preparation method and application of ethanol extract of Cryptocodella sp.
  • A kind of preparation method and application of ethanol extract of Cryptocodella sp.
  • A kind of preparation method and application of ethanol extract of Cryptocodella sp.

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Embodiment 1

[0034] The preparation of the ethanol extract of embodiment 1 Cryptocodella sp.

[0035] S1. Take fresh hydatid algae, freeze at -30°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, and centrifuge at a speed of 8000r / min for 10min. The supernatant and the precipitate were separated, and the precipitate was taken for later use;

[0036] S2. Add ethanol with a volume fraction of 85% to the precipitation described in step S1 according to the solid-to-liquid ratio of wet algae and ethanol of 1 g / mL for ultrasonic extraction 3 times, the frequency of ultrasonic extraction is 40 kHz, and the temperature is 4 ° C. The time is 20min, centrifuged at the speed of 8000r / min for 10min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, the product after vacuum drying is dissolved in first-grade water, and its relative density is adjusted. The weight concentration of the algae (wet algae) is 1 gA / mL (A ...

Embodiment 2

[0038] The preparation of the ethanol extract of embodiment 2 Cryptocodella sp.

[0039] S1. Take fresh hydatid algae, freeze at -20°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 5 times, and centrifuge at a speed of 10,000r / min for 8 minutes. The supernatant and the precipitate were separated, and the precipitate was taken for later use;

[0040]S2. To the precipitation described in step S1, according to the solid-to-liquid ratio of wet algae and ethanol of 1g / mL, add ethanol with a volume fraction of 70% for ultrasonic extraction for 3 times, the frequency of ultrasonic extraction is 35kHz, and the temperature is 4°C. The time is 30min, centrifuged at the speed of 8000r / min for 10min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, the product after vacuum drying is dissolved in first-grade water, and its relative density is adjusted. The weight concentration of the algae (wet algae) is 1 gA...

Embodiment 3

[0041] The preparation of the ethanol extract of embodiment 3 Cryptocodella sp.

[0042] S1. Take fresh hydatid algae, freeze at -40°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 3 times, and centrifuge at a speed of 8000r / min for 15min. The supernatant and the precipitate were separated, and the precipitate was taken for later use;

[0043] S2. To the precipitation described in step S1, according to the solid-liquid ratio of wet algae and ethanol 1g / mL, add ethanol with a volume fraction of 90% for ultrasonic extraction 4 times, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. The time is 15min, centrifuged at 5000r / min for 15min, the supernatant and the precipitate are separated, the supernatant is taken for vacuum drying, the product after vacuum drying is dissolved in first-grade water, and its relative density is adjusted. The weight concentration of the algae (wet algae) is 1 gA / mL (A represents the wet al...

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Abstract

The invention belongs to the technical field of marine organisms, and provides a preparation method and application of ethanol extract of Cryptocodella sp. The ethanol extract of cryptocoleum provided by the invention is obtained by repeatedly freezing and thawing fresh cryptocoleum wet algae, centrifuging to take the precipitate and adding ethanol for ultrasonic extraction, and the obtained cryptocoleum ethanol extract can Significantly improve the activity of superoxide dismutase (SOD) in Caenorhabditis elegans and promote the removal of reactive oxygen species (ROS) in the body, reduce the level of reactive oxygen species (ROS) in the body, and at the same time it can alleviate the oxidative stress caused by premature aging phenomenon, has a significant antioxidant effect, and can be applied to the preparation of health food and medicines with antioxidant functions.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a preparation method and application of ethanol extract of Cryptocodella sp. Background technique [0002] Free radicals refer to atoms, molecules or groups containing unpaired electrons. Since free radicals contain unpaired electrons, they tend to pair and are unstable and highly active. Under normal circumstances, free radicals in the human body are in a dynamic balance of continuous generation and removal. Free radicals are produced too much or cleared too slowly. By attacking life macromolecules and various cells, excess free radicals will cause various damages to the body at the molecular level, cellular level, and tissue and organ level, accelerate the aging process of the body and induce Various diseases, such as free radicals cause DNA oxidative damage, the transformation of cell genetic information, leading to the occurrence of tumors; free radicals...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/105A61K36/02A61P39/06
CPCA23V2002/00A61K36/02A61K2236/333A61K2236/39A61K2236/51A61K2236/53A23L33/105A23V2200/302A23V2200/30A23V2250/202
Inventor 黄泽波卢哲宋立荣胡丽莉余程明
Owner GUANGDONG PHARMA UNIV
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