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Application of disodium ethylenediamine tetraacetate as drug for treating jellyfish dermatitis

A technology for jellyfish and dermatitis, which is applied in the direction of drug combinations, medical preparations containing active ingredients, antipyretics, etc. It can solve the problems of exacerbation of the disease and poor effect, and achieve the effect of broadening the scope of application

Pending Publication Date: 2019-03-15
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no medicines for treating jellyfish dermatitis at home and abroad. Most of them follow folk methods, proven prescriptions or refer to other types of dermatitis for treatment. The effect is poor, and the condition may even be aggravated

Method used

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  • Application of disodium ethylenediamine tetraacetate as drug for treating jellyfish dermatitis
  • Application of disodium ethylenediamine tetraacetate as drug for treating jellyfish dermatitis
  • Application of disodium ethylenediamine tetraacetate as drug for treating jellyfish dermatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take the above-mentioned 10 μM EDTA disodium solution and 50 μg / mL jellyfish toxin in a volume ratio of 1:1, mix evenly, react at 37°C for 30 minutes, and use it as the mixed solution A for use;

[0034] To the plated with 100 μl 1 x 10 5 Add 100 μL of mixed solution A and 100 μL of mixed solution of jellyfish toxin and cell culture medium (control) in a volume ratio of 1:1 to a 96-well plate of human embryonic fibroblast cells (CCC-ESF-1) cells / mL and 100 μL of cell culture medium (blank), after 24 hours of reaction, remove the supernatant, add MTT after 4 hours of reaction, add stop solution, and measure A with a microplate reader within 24 hours 490nm , the reaction was set up in three parallels, and the cell viability was calculated according to the following formula.

[0035]

[0036] The experimental results show (see figure 1), when the mass ratio of disodium EDTA to jellyfish toxin (calculated as protein) was 0.074:1, the survival rates of cells were 64.23...

Embodiment 2

[0038] Take 50 μM EDTA disodium solution and 50 μg / mL jellyfish toxin and mix evenly according to the volume ratio of 1:1, and react at 37°C for 30 minutes, and use it as the mixed solution B for later use;

[0039] To the plated with 100 μl 1 x 10 5 Add 100 μL of mixed solution B and 100 μL of mixed solution of jellyfish toxin and cell culture medium (control) in a volume ratio of 1:1 to a 96-well plate of human embryonic fibroblast cells (CCC-ESF-1) cells / mL and 100 μL of cell culture medium (blank), after reacting for 24 hours, remove the supernatant, add MTT and react for 4 hours, add stop solution, and measure A with a microplate reader within 24 hours 490nm , the reaction was set up in three parallels, and the cell viability was calculated according to the following formula.

[0040]

[0041] The experimental results show (see figure 1 ), when the mass ratio of EDTA disodium to jellyfish toxin (calculated as protein) was 0.37:1, the cell survival rates were 75.41%,...

Embodiment 3

[0043] Take 50 μM EDTA disodium solution and 50 μg / mL jellyfish toxin and mix evenly according to the volume ratio of 1:1, and react at 37°C for 30 minutes, and use it as the mixed solution B for later use;

[0044] To the plated with 100 μl 1 x 10 5 Add 100 μL of mixed solution B, 100 μL of mixed solution of jellyfish toxin and cell culture medium (control) mixed at a volume ratio of 1:1 to a 96-well plate of human keratinocytes (HaCaT) cells / mL, and 100 μL of cell culture Base (blank), after 24 hours of reaction, remove the supernatant, add MTT after 4 hours of reaction, add stop solution, measure A with a microplate reader within 24 hours 490nm , the reaction was set up in three parallels, and the cell viability was calculated according to the following formula.

[0045]

[0046] The experimental results show (see figure 2 ), when the mass ratio of disodium EDTA and jellyfish toxin (calculated as protein) was 0.37:1, the survival rates of cells were 56.81%, 53.52%, 64...

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Abstract

The invention relates to the technical field of marine biology, in particular to application of disodium ethylenediamine tetraacetate (non-specific metalloprotease inhibitor) as a drug for treating jellyfish dermatitis. The invention provides application of EDTA-2Na as a drug for treating jellyfish dermatitis. According to the invention, disodium ethylenediamine tetraacetate can effectively inhibit the death of two types of cells (human keratinocyte HaCaT and human embryonic fibroblast CCC-ESF-1) and the expression amount of an inflammatory factor mRNA caused by physaliatoxin, and further canbe used as a drug for treating jellyfish dermatitis, so that the application range of EDTA-2Na is widened.

Description

Technical field: [0001] The invention relates to the technical field of marine organisms, in particular to the application of disodium edetate (non-specific metalloproteinase inhibitor) as a medicine for treating jellyfish dermatitis. Background technique: [0002] Jellyfish belong to the coelenterate phylum and are marine aquatic animals with cnidarian cells. When people touch jellyfish, the toxins in the cnidarian cells penetrate the skin at a very fast speed and cause stings. The main harmful jellyfish in my country are sand jellyfish and rosy jellyfish. In recent years, jellyfish outbreaks around the world have not only caused disorder in the marine ecosystem, but also seriously affected human production and life. Jellyfish stings have become the most common marine injury. According to estimates by the National Institutes of Health of the United States, about 150 million people are stung by jellyfish every year around the world, and many of them die. Jellyfish dermati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/198A61P29/00A61P17/00
CPCA61K31/198A61P17/00A61P29/00
Inventor 于华华李翱宇李荣锋刘松邢荣娥陈晓琳李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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