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Mixed enzyme production process by solid fermentation of fruits and vegetables and microorganisms

A solid-state fermentation and production process technology, applied in the field of fermentation engineering, can solve problems such as difficulty in entering products, difficulty in controlling the growth of harmful microorganisms, and potential safety hazards.

Inactive Publication Date: 2015-08-19
杭州德润全健康产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymes obtained in this way have the following four problems. First, the enzymes in fruits and vegetables are difficult to enter into the final product. The amount of dissolution depends on the size of the fruit and vegetable pieces.
Second, the enzymes in fruits and vegetables can easily become "food" for fermenting microorganisms, resulting in the loss of fruit and vegetable enzymes
Third, the natural fermentation method is difficult to control the growth of harmful microorganisms
Final product poses a safety hazard
Fourth, the vegetable residue after fermentation is bound to have a certain impact on the environment
But the report that adopts solid-state fermentation to obtain ferment is also seldom at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Choose 500 grams each of non-rotten apples and oranges, clean the apples, and peel the oranges. Place in a juice extractor to extract juice to obtain 789 grams of fruit juice. And 202 grams of oranges and apple pomace, in the oranges and apple pomace, add 50 grams of bran, 100 grams of brown rice flour, and 50 grams of soybean powder. Bacteria 30min. After the temperature dropped to room temperature, each inoculated 15ml of red yeast rice liquid seeds, fermented at 32°C for 3 days, then transferred to 24°C for 20 days, poured out the fermented materials, mixed in apple and orange juice, and then vacuum freeze-dried to obtain Fruit and vegetable and red yeast solid-state fermentation mixed enzyme.

Embodiment 2

[0038] Choose 200 grams each of non-rotten carrots, bitter gourds, oranges, pineapples, and cherries. Wash the bitter gourds and cherries, remove the pits, and peel the oranges and pineapples. Place it in a juice extractor to squeeze the juice to obtain 719 grams of fruit juice. Add 252 grams of fruit and vegetable dregs, add 50 grams of bran, 30 grams of brown rice flour, 30 grams of lily powder, and 30 grams of soybean powder. After mixing evenly, put them into 3 fermentation bottles, and sterilize at 121°C for 30 minutes. After the temperature drops to room temperature, inoculate 17ml of mucormycetes liquid species, ferment at 35°C for 10 days, pour out the fermented material, and mix in apple and orange juice. Then vacuum freeze-drying to obtain fruit and vegetable and mucor solid-state fermentation mixed enzyme.

Embodiment 3

[0040] Choose 200 grams each of non-rotten apples, carrots, bitter gourds, lemons, papayas, and broccoli. Clean the apples and peel the oranges. Place it in a juice extractor to squeeze the juice to obtain 846 grams of fruit and vegetable juice and 339 grams of fruit and vegetable residue. Add 50 grams of bran, 30 grams of brown rice flour, and 50 grams of oat flour to the pomace, mix well, divide into 4 fermentation bottles, and sterilize at 121°C for 40 minutes. After the temperature drops to room temperature, inoculate 12ml of Aspergillus oryzae liquid seed, ferment at 37°C for 15 days, pour out the fermented material, and mix in the fruit and vegetable juice. Then vacuum freeze-dry to obtain the solid-state fermentation mixed enzyme of fruit and vegetable and Aspergillus oryzae.

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PUM

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Abstract

The present invention discloses a mixed enzyme production process by solid fermentation of fruits and vegetables and microorganisms and the production process includes the following steps: step one, washing and cleaning fresh fruits and vegetables; step two, juicing the cleaned fruits and vegetables using a juice extractor and obtaining the fruit and vegetable juice and the fruit and vegetable residue; step three, adding water content adjusting raw materials according to different water content of the obtained fruit and vegetable residue to adjust the water content of the fruit and vegetable residue, and mixing the above materials evenly to obtain a base material; step four, putting the step three obtained base material into a fermentation bottle to conduct sterilization; step five, cooling the sterilized fruit and vegetable base material to room temperature, and inoculating microorganism liquid strains to conduct solid fermentation; and step six, after the end of solid fermentation, blending the obtained fruit and vegetable juice into the solid fermented materials, conducting low temperature drying, and thereby obtaining the fruit and vegetable and microorganism mixed enzymes containing both fruit and vegetable enzymes and microbial enzymes. The mixed enzymes can not only fully preserve the enzyme activity of fruits and vegetables, but also obtain the enzyme ingredients of microorganisms, and in addition, the production process can also achieve the full advantage of the entire fruits and vegetables, does not produce any waste, and is ecological and environmental protective.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a production process of fruit and vegetable solid-state fermentation mixed enzymes. Background technique [0002] Enzyme, a protein with catalytic activity, is essentially an enzyme, which is a catalyst for all biochemical reactions in organisms, and the human body is no exception. According to the different sources of enzymes, enzymes derived from plants are usually called plant enzymes, such as papain from papaya, bromelain from pineapple, etc., all belong to plant enzymes; enzymes derived from animals are called animal enzymes, such as animal pancreas from sources Trypsin, pepsin extracted from gastric juice, etc.; microbial enzymes derived from microorganisms, such as amylase and pectinase derived from Aspergillus niger. [0003] As a catalytically active protein, an enzyme has the following properties: [0004] (1) The vast majority of enzymes are sensitive to heat,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L33/00
Inventor 陈小林严杰凯赵敏邵丽芳
Owner 杭州德润全健康产业发展有限公司
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