Method for oriented biosynthesis of GAMG

A biosynthesis and seed technology, applied in the biosynthesis of monoglucuronic acid glycyrrhetinic acid, and the field of directional catalysis of glycyrrhizic acid to synthesize GAMG, can solve the problems of poor catalysis orientation, easy environmental pollution, and low chemical synthesis selectivity, Achieve the effect of low cost, single product and high reaction efficiency

Inactive Publication Date: 2009-12-16
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of this invention is to provide a kind of method of directional biosynthesis monoglucuronyl glycyrrhetinic acid (GAMG), to over

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1: Biosynthesis of GAMG

[0020] Seed medium: each liter of medium contains 5g glycyrrhizic acid, 3g glucose, NH 4 NO 3 3g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.001g, sterilized at 121℃ for 20min;

[0021] Fermentation culture: each liter of medium contains 5g glycyrrhizic acid, NH 4 NO 3 3g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.001g, sterilized at 121℃ for 20min;

[0022] Transformation solution: 10g glycyrrhizic acid, 0.05M pH 7.0 phosphate buffer at a constant volume of 500ml.

[0023] The slant strain producing Penicillium violaceum was connected to the seed culture medium, and cultured in a shaker at 30°C for 24h at 180r / min. The seed liquid was transferred to the fermentation medium with a volume ratio of 1:10 inoculum, cultured on a shaker at 28°C and 180r / min for 96 hours to obtain a fermentation broth. The fermentation broth was added to the conversion broth at a ratio of 50%. The concentration of glycyrrhiz...

Example Embodiment

[0024] Example 2: Biosynthesis of GAMG

[0025] Seed medium: 0.5g glycyrrhizic acid, 1g glucose, NH 4 NO 3 3g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.001g, distilled water 1000mL, 121℃ sterilized for 20min;

[0026] Fermentation medium: glycyrrhizic acid 0.5g, NH 4 NO 3 3g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.001g, distilled water 1000mL, 121℃ sterilized for 20min;

[0027] Transformation solution: 8g glycyrrhizic acid, 0.05M pH 7.0 phosphate buffer solution with a constant volume of 500ml.

[0028] The penicillium violaceum-producing slant strain was connected to the seed culture medium, and cultured on a shaker at 25°C for 20 hours at 100 r / min. The seed liquid is transferred to the fermentation medium at a volume ratio of 1:10 inoculum, cultured in a shaker at 25°C and 100r / min for 72 hours to obtain a fermentation broth. The fermentation broth is added to the conversion broth at a ratio of 30%. The concentration of glycyrrhizic ...

Example Embodiment

[0029] Example 3: Biosynthesis of GAMG

[0030] Seed medium: glycyrrhizic acid 8g, glucose 5g, NH 4 NO 3 3g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, 1000mL distilled water, 121°C sterilization for 20min;

[0031] Fermentation medium: Glycyrrhizic acid 8g, NH 4 NO 3 3g, KH 2 PO 4 1g, MgSO4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, 1000mL distilled water, 121°C sterilization for 20min;

[0032] Transformation solution: glycyrrhizic acid 40g, 0.05M pH 7.0 phosphate buffer solution with a constant volume of 500ml.

[0033] The slant strain producing Penicillium violaceum was connected to the seed culture medium, and cultured on a shaker at 40°C for 24h at 300r / min. The seed liquid was transferred to the fermentation medium with a volume ratio of 1:10 inoculum. The fermentation broth was obtained by culturing at 40°C and 150r / min in a shaker for 96 hours. The fermentation broth was added to the conversion broth at a ratio of 50%. The concentration of glycy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for oriented biosynthesis of GAMG, and belongs to the technical fields of biosynthesis and biotransformation. The method comprises the following steps: producing corresponding beta-D-glucuronidase by penicillium purpurogenum stoll in a fermentation medium; adding the obtained beta-D-glucuronidase into conversion solution containing glycyrrhizic acid substrate, orientedly hydrolyzing the glycyrrhizic acid under the action of enzyme, and obtaining a coarse product of GAMG; and performing organic solvent extraction, resin adsorption and purification to obtain a GAMG product. The method takes microbialenzyme as a catalyst, and has high reaction efficiency, no pollution, simple steps, and low cost; and the product is single and has high purity.

Description

technical field [0001] The invention relates to a biosynthetic method of monoglucuronyl glycyrrhetinic acid (GAMG), in particular to a method for directionally catalyzing glycyrrhizic acid to synthesize GAMG under the action of microbial enzymes, and belongs to the technical field of biosynthesis and biotransformation. Background technique [0002] Licorice is a leguminous plant of the genus Glycyrrhizae. Glycyrrhizic acid is one of the main active ingredients in licorice, which has anti-inflammatory, anti-virus, anti-tumor, and anti-digestive ulcer effects. In addition, glycyrrhizic acid is also an important natural sweetener, its sweetness is about 200 times that of sucrose, and its calories are low, so it is widely used as a food additive. [0003] Glycyrrhizin (GL) is one of the main active ingredients of the traditional Chinese medicine licorice. It consists of two glucuronic acids connected by pentacyclic triterpene saponins through β-1,2 glycosidic bonds and β-1,3 gl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/56C12R1/80
Inventor 李春王小艳邹树平冯世江戴大章
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap