32 results about "Penicillium purpurogenum" patented technology

The invention discloses a preparation method for seawater microbial flocculants. In the invention, penicillium purpurogenum separated and sieved from coast intertidal zone sludge is adopted to prepare the seawater microbial flocculants; potato juice is taken as raw materials to prepare fermentation medium; penicillium purpurogenum EL-O2 strains are inoculated in the fermentation medium, and are cultured at 25-35DEG C at shaker rotating speed of 160-200rpm to obtain ferment culture solution; the ferment culture solution is centrifugalized at a high speed under the conditions that the temperature is 4-8DGE C and the rotating speed is 8000-12000rpm to obtain ferment liquid supernatant; the ferment liquid supernatant is precipitated with ethanol and is centrifugalized at a high speed at 4-8DEG C and at the rotating speed of 10000-12000rpm to obtain precipitate; and the precipitate is subject to vacuum freeze-drying under the conditions that the temperature is 45DEG C below zero to 20DEG C below zero and the vacuum degree is lower than 15pa to obtain the finished seawater microbial flocculants. The seawater microbial flocculants having high flocculation activities and being specially used for sea water processing have ideal flocculation effect and simple process, and are convenient to apply and prompt.

The invention discloses an anti-bacterial mould-proof floor film and a preparation technology thereof. The floor film is prepared from the following raw materials in parts by weight: 80-100 parts of PVC resin powder, 2.5-4 parts of a silver-carrying nano TiO2 antibacterial agent, 2-3 parts of a mildew preventive, 12-16 parts of epoxidized soybean oil, 5-7 parts of a plasticizer and 3-5 parts of a stabilizer. The raw materials of the floor film are added with the silver-carrying nano TiO2 antibacterial agent with good antibacterial property, breeding of microorganisms such as bacteria can be effectively inhibited, and a floor is prevented from being eroded by the bacteria; and the mildew preventive in the raw materials is a compound of pyridine sulfur-copper-zinc and N-octyl-4-isothiazolin-3-one, can inhibit growth of gram positive and negative bacteria, penicillium purpurogenum and aspergillus fumigatus and has excellent mildew-proof property.

The invention discloses a process and device for continuously producing glycyrrhetinic acid monoglucuronide by converting glycyrrhizic acid by using immobilized cells of Li-3(Penicillium purpurogenum Li-3) CGMCC NO.5446 and belongs to the field of biochemical engineering. According to the process, penicillium purpurogenum Li-3 is immobilized by using diatomite and calcium alginate, and the glycyrrhetinic acid monoglucuronide is continuously produced by converting the glycyrrhizic acid in a packed bed reactor with an isolation screen by using the immobilized cells. The packed bed reactor with the isolation screen is manufactured by adding an isolation screen into a common packed bed reactor so as to load beads of the immobilized cells via different layers of a packed bed so that damage caused by the self-weight of the beads is reduced; by adopting the process and the device, the activity of the cells can be kept for a long time, the service life of the beads of the immobilized cells is prolonged, the product purity is improved, the production cost is greatly reduced, and large-scale continuous production of glycyrrhetinic acid monoglucuronide is possible.

The invention relates to a method for preparing fibrilia by using penicillium purpurogenum DB1 strains. The method comprises the following steps: (1) preserving the penicillium purpurogenum stoll DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria, the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.

The invention belongs to the field of medical chemical engineering and relates to a chromone dipolymer derivative as well as a preparation method and application thereof. Specifically, the invention relates to a compound with formula I, wherein S and R respectively represent absolute configurations of corresponding marking carbon atoms; R1-R3 independently represent various substituent groups, respectively; R3 and H connected by a wavey line can orientate alpha or beta. The invention further relates to penicillium purpurogenum of the compound with the formula I. Experiments prove that the compound with the formula I has good anti-tumor activity, can be used for preparing biological reagents such as a tumor cell killing agent, a tumor cell proliferation inhibitor and an anti-tumor agent, and further can be used for preparing anti-tumor and anti-cancer drugs. The formula I is as shown in the specification.

The invention relates to a penicillium purpurogenum DB1 strain and preparation and application thereof. A preparation method of the strain comprises the following steps of: (1) putting rotten seaweed into an enriched medium for stationary culture at room temperature, coating the enriched medium onto an isolation medium, and stationary culture for 1-4 days at 30 DEG C to obtain a wild flax biological treatment strain; (2) inoculating the strain obtained in the step (1) into a flax lignin enriched medium, and culturing at 28 DEG C for 72 hours; and (3) selecting a strain with lignin degrading capability from the flax lignin enriched medium to obtain the penicillium purpurogenum DB1 strain. The penicillium purpurogenum DB1 strain can be applied to preparation processes of flax, hemp, jute orred jute fiber for spinning by degumming with peroxides. The strain has the advantages of short growing period, difficultly polluted, low treatment cost, good fiber quality after treatment, mild reaction condition, high heat resistance and environmental friendliness; and the strain has a simple process and is suitable for mass industrial production.

The invention relates to a method for preparing a Beta-glucuronidase crude enzyme preparation, and belongs to the field of biotechnology (fermentation engineering). The method comprises the following steps: adding an exogenous accelerant into an enzyme production culture medium containing glycyrrhizic acid or glycyrrhetate, wherein the exogenous accelerant is total liquorice extractives, or total liquorice polysaccharides and/or total liquorice flavone; promoting penicillium purpurogenum Li-3 (Collection No.: CGMCC No. 5446) of strains to induce the generation of Beta-glucuronidase. Compared with the method without the accelerant adding step, the method has the advantages that glucuronidase is generated 5 to 48 hours earlier, and the activity of glucuronidase is improved by 10 to 200 percent.

The invention discloses penicillium purpurogenum for poisoning plant parasitic nematodes and a preparing method and application thereof. The bacterial strain number of the penicillium purpurogenum for poisoning plant parasitic nematodes is MHZ111, and the register number of the penicillium purpurogenum for poisoning plant parasitic nematodes in the China General Microbiological Culture Collection Center is CGMCC No.11630. A plant parasitic nematode inhibitor capable of being dissolved in ethyl acetate is a substance extracted from a penicillium purpurogenum MHZ111 culture by means of ethyl acetate. The root-knot nematode second-stage juvenile killing ability is obviously higher than that of abamectin and fosthiazate when the contents of active ingredients are 1.5 g/L and 1.0 g/L respectively. Incubation of root-knot nematodes can hardly be achieved when the contents of the active ingredients are both 1.5 g/L. The root-knot nematode oocyst incubation restraining effect is similar to that of abamectin and fosthiazate when the contents of active ingredients are 1.0 g/L and 1.5 g/L respectively.

The invention provides a process for preparing ceramide by a fermentation method. The process comprises the technical flow processes of preparation of a culture medium, culture of penicillium purpurogenum EFOZ-02, sample preparation, HPLC (High Performance Liquid Chromatography) separation, drying and the like. When the process provided by the invention is used for producing the ceramide, the penicillium purpurogenum EFOZ-02 can secrete a great number of ceramide type substances into fermentation liquid, so that an any derivatization process is not needed; the operation is simple; the extraction is convenient; the microbial fermentation period is short; the effect taking speed is high; the limitation by climates in districts is avoided; importantly, in reported studies about the penicillium purpurogenum at prevent, the existence of the existing toxin in metabolites is not discovered; certain safety is ensured; by using the process provided by the invention for producing the ceramide, the economic cost can be greatly reduced; and the large-scale industrial production of the ceramide can be promoted. Therefore the process has huge market application values.

The invention belongs to the field of microbiology, in particular to a penicillium strain which can efficiently degrade wastewater and ammonia nitrogen in odor, and the application in treatment of sewage and odor pollution thereof. Penicillium, also Penicillium purpurogenum by classification, has been preserved in the China General Microbiological Culture Collection Center (CGMCC), CGMCC No. 8036. The bacterial strain has the advantages of efficiently degrading wastewater and ammonia nitrogen in odor, and efficiently treating sewage and odor pollution.

The invention belongs to the field of biological pesticides, and discloses a litchi endogenetic Penicillium purpurogenum which is prepared by taking litchi endogenetic microorganisms as screening objects through separating and has a significant inhibition effect on litchi downy blight, as well as a biological preparation and an application thereof in controlling litchi downy blight. The Penicillium purpurogenum, with a Preservation Number of CCTCC NO:M2013738, is named 'Penicillium purpurogenum' GY-19, preserved in China Center for Type Culture Collection (CCTCC) on December 31, 2013 at Wuhan University, Wuhan, China. The strain is from litchi endogenetic fungi, can be colonized on the surfaces of litchi fruits well, and has a strong inhibition effect on litchi downy blight, therefore, the Penicillium purpurogenum has an excellent biocontrol effect on controlling litchi downy blight; and the Penicillium purpurogenum is environment-friendly and non-toxic in biogenetic derivation and low in culture conditions, and has a small influence on the ecological environment, therefore, the Penicillium purpurogenum has an extremely good development prospect.

The invention belongs to the field of pharmaceutical chemicals, and relates to drimane-type sesquialter terpene cyclohexenone derivative, a preparation method thereof and application. Particularly, the drimane-type sesquialter terpene cyclohexenone derivative relates to a compound in a formula I, wherein Arabic numerals in the formula I indicate scalar potentials, and an R and an S respectively represent absolute configurations of carbon atoms with the corresponding potentials. The invention further relates to the preparation method of the compound, a composition containing the compound and the anti-tumor application. The invention further relates to penicillium purpurogenum BD-1-6 capable of being used for preparing the compound. The compound in the formula I can effectively kill tumor cells or suppress proliferation of the tumor cells, has excellent anti-tumor activity and has the potential of being used as an anti-tumor medicine. Attached is the formula I.

The invention provide a fermentation medium and method for improving the activity of beta-glucuronidase produced by fungi. The fermentation medium is a solution prepared by filtering boiled potatoes and water and adding a promoter and water, wherein the promoter is glycyrrhizic acid or a salt thereof. According to the fermentation medium and method provided by the invention, Penicillium purpurogenum Li-3, Talaromyces pinophilum Li-93, Aspergillus terreus Li-20 and Aspergillus ustus Li-62 are subjected to fermentation and culture for production of beta-glucuronidase; and it is found that the fermentation enzyme activity unit of each fungus is increased by 100 to 600% and fermentation time is brought forward by 36 to 60 h compared with the prior art. Thus, the fermentation medium and method provided by the invention have the characteristics of simple operation, low cost, high fermentation enzyme activity unit and short enzyme production time.

The invention belongs to the field of medical and chemical industry, relates to indoline diketopiperazine spiro-compounds as well as a preparation method and use thereof, and in particular relates to compounds as shown in formula I or pharmaceutically acceptable salts of the compounds. The compounds have a framework dimerization molecular structure condensed by terracycloindoline diketopiperazine and 1-oxa spiro[4, 5] decane. Two ethylene oxide structures are arranged on the spiro hexatomic ring. The invention further relates to penicillium purpurogenum for preparing the compounds as shown in the formula I. Experiments show that the compounds can be used for preparing cytoskeletal protein inhibitors, apoptosis inducers, tumor cell proliferation inhibitors, tumor cell killing agents or antitumor drugs. The compounds provided by the invention have good antitumor activity.

The invention belongs to the field of pharmaceutical chemical industry and relates to a lipopeptide compound as well as compositions, a preparation method and application thereof, in particular to a compound I or pharmaceutically acceptable salts thereof, wherein R represents hydroxymethyl, C1-10 linear chain or branch chain alkoxy, C2-10 linear chain or branch chain alkenyloxy, C2-10 linear chain or branch chain alkynyloxy, C6-10 linear chain or branch chain aryloxy or C2-18 saturated or unsaturated fat acyloxy methyl. The invention also relates to penicillium purpurogenum for preparing the compound. Tests prove that the compound can be used for preparing a lethal cytotoxin reagent or an antitumor agent on tumor cells. The compound disclosed by the invention has favorable antitumor activity.

The invention relates to the technical field of pear tree rot disease treatment, and discloses a method for preventing and treating rot disease by using endophyte. The method comprises the following steps: S1, preparing penicillium purpurogenum; S2, preparing sterile filtrate; S3, preparing a virus-carrying flat plate; S4, selecting pear tree branches; S5, punching holes in the branches by using a puncher, wherein the hole reaches the xylem; and S6, after treatment, placing the branches in an incubator to be subjected to moisture preservation treatment. According to the invention, a fungus JK2 is separated from a pear tree branch having rot disease, the fungus JK2 is identified as penicillium purpurogenum through morphology and molecular biology, a plate confrontation test shows that the fungus JK2 has strong inhibitory activity on pear tree rot fungi, the inhibition rate reaches 95% or above, an in-vitro pear tree branch identification result shows that the JK2 has a good prevention and treatment effect on the pear tree rot disease, and the penicillium purpurogenum is an endophytic fungus, is harmless to the environment and human bodies, and is beneficial to development of biopesticides.