Method for preparing fibrilia by using penicillium purpurogenum DB1 strains

A technology for producing Penicillium purpura and hemp fibers, which is applied in chemical post-treatment of fibers, methods based on microorganisms, biochemical equipment and methods, etc., and can solve the problems of high energy consumption in the chemical degumming process, inability to recycle waste water, and large loss of equipment And other problems, to achieve good results, short degumming cycle, short production process

Inactive Publication Date: 2012-04-11
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical degumming process consumes a lot of energy and equipment, and th

Method used

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  • Method for preparing fibrilia by using penicillium purpurogenum DB1 strains
  • Method for preparing fibrilia by using penicillium purpurogenum DB1 strains
  • Method for preparing fibrilia by using penicillium purpurogenum DB1 strains

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Screening and Obtaining of Flax Degumming Strains

[0041] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea. Firstly, seaweed and enrichment medium were cultured in the enrichment medium at room temperature for 30 days at a ratio of 1g: 20mL, and then 0.1mL of enrichment medium was applied to the separation medium. Culture medium, static culture at 30°C for 1 to 4 days, to obtain the dominant strain of wild-type flax biotreatment;

[0042] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 mL of tap water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g,K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 7-7.2 with NaOH;

[0043] (2) Inoculate the bacterial strain obtained in the step (1) into flax lignin nutrient medium, and culture at 28° C. for 72 hours. Flax lignin nutrient medium, its components include: fla...

Embodiment 2

[0046] Preparation of fermentation broth:

[0047] (1) The Penicillium purpurea DB1 strain was stored in potato dextrose agar medium, cultured at 30°C and 220 rpm for 48 hours, and added freezing buffer; the freezing buffer consisted of 0.0627 g of potassium dihydrogen phosphate, 0.0177 g of dipotassium hydrogen phosphate, citric acid Prepared by adding 0.0588g of sodium, 0.02645g of magnesium sulfate heptahydrate, 10mL of glycerin, and distilling to 100mL with water.

[0048] (2) In the sterilized and cooled 50mL potato dextrose medium, insert the bacteria ring obtained in step (1), and shake the flask for 48 hours at a rotation speed of 220r / min and a temperature of 30°C;

[0049] (3) Inoculate the culture medium obtained in step (2) into 5 L of flax lignin fermentation medium, and shake the flask for 48 hours at a rotation speed of 220 r / min and a temperature of 30° C. to obtain a fermentation broth; wherein, the formulation of the flax fermentation medium is: : Flax raw h...

Embodiment 3

[0051] The flax degumming process is as follows:

[0052] Put 5g of the original flax stem into a 250mL conical flask, divide and pack in the conical flask with the original flax stem and the fermentation broth of Example 2 at a bath ratio of 1:20, the liquid volume is 100mL, at 40°C, in a shaker at 200rpm Respectively degumming for 1 day; when the degumming time is up, remove the degumming solution and stop the biological treatment. The biologically treated raw flax stems are inactivated, and the process is as follows: the biologically treated flax raw stems are mixed with the degumming solution at a ratio of 1:10, and treated at 70°C for 3 hours, wherein the peroxide The consumption of sodium percarbonate is 3% of the original stem weight of flax, the consumption of urea is 0.05% of the original stem weight of flax, the consumption of sodium tripolyphosphate is 2% of the original stem weight of flax, and the consumption of disodium edetate is 0.1% by weight of the original ...

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Abstract

The invention relates to a method for preparing fibrilia by using penicillium purpurogenum DB1 strains. The method comprises the following steps: (1) preserving the penicillium purpurogenum stoll DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria, the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of preparing hemp fiber by using fungi, and in particular relates to a method for preparing hemp fiber by using the Penicillium purpurea DB1 strain. Background technique [0002] Hemp is one of the raw materials for textiles, and its varieties mainly include flax, ramie, hemp, jute, kenaf, apocynum, sisal, abaca, nettle and velvetle. Hemp fiber is a general term for fibers obtained from various hemp plants. It has the incomparable advantages of other natural fibers such as moisture absorption, breathability, heat dissipation and antibacterial. As one of the countries with the richest hemp resources in the world, my country produces hemp fiber and its products not only to meet the needs of the domestic market, but also exported to the United States, Western Europe and Southeast Asian countries as bulk commodities, with an average annual foreign exchange earning of nearly 10 billion U.S. dollars. Driven by the national...

Claims

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Application Information

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IPC IPC(8): D01C1/00C12N1/14C12S11/00C12R1/80
Inventor 丁若垚刘国亮董政娥黎征帆郁崇文张兴群
Owner DONGHUA UNIV
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