Process and device for continuously producing glycyrrhetinic acid monoglucuronide by using immobilized cells

A technology of immobilizing cells and glycyrrhetic acid is applied in biochemical cleaning devices, enzymology/microbiology devices, microorganism-based methods, etc., which can solve the problems of complicated separation and purification steps, many impurities in products, and high costs, and achieve Long service life, high product purity and energy saving effect

Inactive Publication Date: 2014-06-18
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the catalysts used to convert glycyrrhizic acid into monoglucuronoglycyrrhetinic acid by biological methods are mainly microbial cells or enzymes extracted from microbial cells, but there are ofte

Method used

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  • Process and device for continuously producing glycyrrhetinic acid monoglucuronide by using immobilized cells
  • Process and device for continuously producing glycyrrhetinic acid monoglucuronide by using immobilized cells
  • Process and device for continuously producing glycyrrhetinic acid monoglucuronide by using immobilized cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Fabrication of packed bed reactors

[0025] The packed bed reactor provided in this example is a plexiglass column with an inner diameter of 50 mm, a height of 500 mm, a reactor volume of 981 mL, a diameter of the isolation sieve chassis of 48 mm, and a support column height of 50 mm.

Embodiment 2

[0027] (1) Preparation of Penicillium purpurea Li-3CGMCC NO.5446 cells

[0028] Inoculate the Penicillium purpurea Li-3 cultured on the slant into the seed medium, cultivate at 32°C, 170r / min for 72h, and then transfer to the secondary seed medium at 32°C, 170r / min with an inoculation amount of 1.5% Cultivate for 24 hours to obtain secondary seed liquid. The cultivated secondary seed culture solution was inoculated into a 1000mL Erlenmeyer flask containing 300mL fermentation medium at an inoculum volume of 10% (v / v) for fermentation at a fermentation temperature of 30°C and a rotational speed of 170r / min. The obtained Penicillium purpogenum Li-3 was centrifuged at 12000 rpm for 10 min, and then the thalline was washed 3 times with distilled water.

[0029] The composition of the seed liquid medium is 5.0g glucose and 3.0g NaNO per liter of water 3 , 1.0g K 2 HPO 4 , 0.5gMgSO 4 ·7H 2 O, 0.5g KCl, 0.01g FeSO 4 ·7H 2 O; The composition of the fermenter medium is 4.5g glyc...

Embodiment 3

[0037] (1) The preparation of Penicillium purpogenum Li-3CGMCC NO.5446 cells was the same as described in Example 2.

[0038] (2) Preparation of immobilized Penicillium purpogenum Li-3CGMCC NO.5446

[0039] Add distilled water to prepare 20% bacterial suspension for the fermented bacteria, add 1% diatomaceous earth, stir evenly, and mix with twice the volume of 2.5% sodium alginate solution sterilized at 121°C for 20 minutes. Draw the suspension of sodium alginate bacteria with a syringe, drop into 2% CaCl on a magnetic stirrer running at low speed 2 Immobilize in the solution to form immobilized gel beads of uniform size, the maximum difference in diameter ≤ 0.5mm, immobilize for 12 hours, and prepare gel beads with a diameter of 3mm, and store them in 0.9% physiological saline in a refrigerator at 4°C middle.

[0040] (3) Packing of immobilized cells into a column is the same as described in Example 2.

[0041] (4) Production of monoglucuronyl glycyrrhetinic acid by conti...

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Abstract

The invention discloses a process and device for continuously producing glycyrrhetinic acid monoglucuronide by converting glycyrrhizic acid by using immobilized cells of Li-3(Penicillium purpurogenum Li-3) CGMCC NO.5446 and belongs to the field of biochemical engineering. According to the process, penicillium purpurogenum Li-3 is immobilized by using diatomite and calcium alginate, and the glycyrrhetinic acid monoglucuronide is continuously produced by converting the glycyrrhizic acid in a packed bed reactor with an isolation screen by using the immobilized cells. The packed bed reactor with the isolation screen is manufactured by adding an isolation screen into a common packed bed reactor so as to load beads of the immobilized cells via different layers of a packed bed so that damage caused by the self-weight of the beads is reduced; by adopting the process and the device, the activity of the cells can be kept for a long time, the service life of the beads of the immobilized cells is prolonged, the product purity is improved, the production cost is greatly reduced, and large-scale continuous production of glycyrrhetinic acid monoglucuronide is possible.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a process for continuously producing monoglucuronoglycyrrhetinic acid by using immobilized cells of Penicillium purpurationum Li-3CGMCC NO.5446 to convert glycyrrhizinic acid and a device used therein. Background technique [0002] Glycyrrhetinic acid monoglucuronide (GAMG) is a derivative of glycyrrhizin (GL) modified to remove a glucuronic acid group. It is a natural functional sweetener with high sweetness and low calories. , the sweetness is more than 1000 times that of sucrose. Monoglucuronyl glycyrrhetinic acid is stable in nature, and has the advantages of remarkable high temperature resistance, acid resistance and high pressure resistance, sweetness hysteresis, good emulsification and foaming properties. Glycyrrhetinic acid monoglucuronide not only retains the anti-inflammation, anti-virus, and anti-tumor effects of glycyrrhizic acid, but because of its moderate polarit...

Claims

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Application Information

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IPC IPC(8): C12P19/56C12M1/00C12R1/80
Inventor 李春肖玉清黄申吕波戴大章
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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