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A strain of Penicillium lavender that decomposes insoluble aluminum phosphate in acidic soil and its application

A technology of Penicillium purpuricum and acid soil is applied in soil remediation and biological fields to achieve the effect of improving the utilization efficiency of phosphate fertilizer, increasing the yield and increasing the content

Inactive Publication Date: 2019-04-26
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a species of Penicillium, there are few reports on its ability to solubilize phosphorus, especially insoluble phosphorus in acidic soils.

Method used

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  • A strain of Penicillium lavender that decomposes insoluble aluminum phosphate in acidic soil and its application
  • A strain of Penicillium lavender that decomposes insoluble aluminum phosphate in acidic soil and its application
  • A strain of Penicillium lavender that decomposes insoluble aluminum phosphate in acidic soil and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preliminary screening of embodiment 1 phosphorus-solubilizing microorganism

[0019] Weigh 10g of water-soaked feces and biogas residue provided by the pig farm of Wuhan Tianjian Agricultural Development Co., Ltd. into a triangular flask filled with 90mL of sterile saline, and shake at a constant temperature of 180r / min on a constant temperature shaker at 28°C for 30min to disperse the sample evenly In distilled water, stand for 20 minutes and take the supernatant to obtain a dilution factor of 10 -1 Bacterial suspension, diluted sequentially to obtain 10 -3 、10 -5 、10 -7 Bacterial suspension of double dilution, respectively take 0.1ml of bacterial suspension of each concentration gradient and spread evenly on the solid medium containing aluminum phosphate (AlPO 4 , 5g / L), each concentration was repeated three times, placed in a constant temperature incubator, and incubated at a constant temperature of 30°C for 5-7 days. Observe the colonies grown on the medium,...

Embodiment 2

[0020] The re-screening of embodiment 2 phosphorus-solubilizing microorganisms

[0021] The strains obtained from the primary screening were inoculated into 100 mL of inorganic phosphorus liquid medium containing aluminum phosphate (AlPO 4 , 5g / L), 30°C, 180rpm, cultivated for 15 days, and at the same time, the liquid phosphate-dissolving medium without adding any bacteria was used as a control group. After the culture solution was centrifuged at 5000r / min for 10min, the supernatant was taken, diluted to an appropriate concentration, and the content of soluble phosphorus was determined by the molybdenum-antimony anti-colorimetric method. The result is as figure 1 Shown, above-mentioned 6 bacterial strains screened by the present invention all have certain phosphorus-dissolving effect, can remove insoluble phosphorus (AlPO) in the culture medium 4 ) into soluble phosphorus, among which the PA01 strain had the highest phosphorus-dissolving efficiency, and the dissolved phospho...

Embodiment 3

[0022] Identification of embodiment 3 bacterial strain PA01

[0023] (1) Colony morphology of strain PA01

[0024] The colonies are white, velvety in texture, with round and neat edges; the colonies are colorless on the back or slightly yellow in the center. There are a large number of conidia on the front of the colony, with light yellow exudate; the flora is white, the hyphae are developed, the conidiophores are tubular, and conidia are produced on them, and the conidia are round.

[0025] (2) Molecular biological identification of strain PA01

[0026] The PA01 strain obtained from the above screening was identified by molecular biology methods, and the 18SrDNAITS sequence of the strain was amplified by PCR to obtain an amplified product with a length of about 600 bp. The amplified product was sequenced by a sequencing company, and the measured sequence was compared with The sequence in the GenBank database was compared by BLAST, and the result showed that the similarity b...

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Abstract

The invention discloses a strain of Penicillium janthinellum PA01 for decomposing insoluble aluminum phosphate in acidic soil, which is preserved in China Center for Type Culture Collection with the preservation number CCTCC NO: M2015757. The bacterial strain provided by the present invention enriches the genetic resources of wild phosphate-solubilizing bacteria, expands the reserve pool for understanding the whole genome breeding of phosphate bacteria, and the bacteria can increase the content of available phosphorus in acidic soil, improve the utilization efficiency of phosphorus fertilizer, increase the yield of crops, and reduce soil erosion ,with broadly application foreground.

Description

technical field [0001] The invention belongs to the field of soil remediation and biotechnology, in particular to a strain of Penicillium janthinellum PA01 and its dissolving effect on insoluble aluminum phosphate, which can be applied to dissolve insoluble phosphorus in acidic soil. technical background [0002] Phosphorus plays an important role in plant growth and development, but most of the phosphorus in soil cannot be directly absorbed by plants. According to the survey, 74% of the cultivated land in the country is deficient in phosphorus. In order to meet the production needs, most of the farmland in my country needs to apply a large amount of phosphorus fertilizer, but the seasonal utilization rate of the applied phosphorus fertilizer is only 5%-25%. Most of the phosphorus and soil Ca in 2+ , Fe 3+ 、Al 3+ etc. combined to form insoluble phosphate, which cannot be absorbed and utilized by plants. In acidic soil, it mainly exists in the form of Al-P and Fe-P. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/80C09K109/00
CPCC09K17/14C09K2109/00C12N1/145C12R2001/80
Inventor 朱端卫李震宇肖乃东周洋肖雪周文兵蔡建波赵建伟万小琼刘广龙华玉妹
Owner HUAZHONG AGRI UNIV
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