Method for cultivating plant with changed drought resistance, ZmMADS27 protein and coding gene thereof

A drought-resistant, plant-based technology, applied in the biological field, can solve problems such as rapid water loss, inter-annual instability, and large evaporation, and achieve the effects of improving drought resistance, significant application and promotion value, and rapid breeding

Active Publication Date: 2022-07-01
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The annual precipitation in these areas ranges from 200-600 mm. In some places, the evaporation is large, the water loss is fast, and the precipitation is highly variable. The growth and development of corn has a low satisfaction rate of water needs and is unstable between years.

Method used

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  • Method for cultivating plant with changed drought resistance, ZmMADS27 protein and coding gene thereof
  • Method for cultivating plant with changed drought resistance, ZmMADS27 protein and coding gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Discovery of ZmMADS27 protein and its encoding gene

[0043] A new protein was discovered from maize B73, as shown in SEQ ID NO: 1 of the sequence listing, and named as ZmMADS27 protein.

[0044] In the genomic DNA of maize B73, the ZmMADS27 gene is shown in SEQ ID NO: 2 (9310 bp) of the sequence listing. In sequence 2, the reading frame of the transcript is nucleotides 794-8284, including 8 exons. The 8 exons are: nucleotides 1-185 of the reading frame, nucleotides 2636-2714, nucleotides 3324-3418, nucleotides 3866-3965, nucleotides 6205- Nucleotides 6246, nucleotides 6374-6415, nucleotides 7164-7327, nucleotides 7443-7491.

Embodiment 2

[0045] Example 2. Acquisition of transgenic plants and identification of drought resistance

[0046] 1. Construction of recombinant plasmids

[0047] The DNA molecule shown in sequence 3 of the sequence listing was inserted into the pBCXUN vector to obtain a recombinant plasmid pBECXUN-ZmMADS27, which was verified by sequencing. In the recombinant plasmid pBECXUN-ZmMADS27, the transcription of the DNA molecule shown in SEQ ID NO: 3 of the sequence listing is initiated by the Ubi promoter and terminated by the Nos terminator, thereby expressing the ZmMADS27 protein.

[0048] Second, the acquisition of ZmMADS27 gene overexpressing plants

[0049] 1. The recombinant plasmid pBECXUN-ZmMADS27 was introduced into Agrobacterium EHA105 to obtain a recombinant Agrobacterium.

[0050]2. Use the recombinant Agrobacterium prepared in step 1 to infect the embryogenic callus of maize B73, and then perform co-cultivation and resistance screening in sequence (the herbicide glufosinate is us...

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Abstract

The invention discloses a method for cultivating a plant with changed drought resistance, a ZmMADS27 protein and a coding gene of the ZmMADS27 protein. The protein provided by the invention is named as ZmMADS27 protein and is a protein shown as a sequence 1 in a sequence table. The gene for coding the ZmMADS27 protein also belongs to the protection range of the invention, and the gene is named as the ZmMADS27 gene. The invention also protects the application of the ZmMADS27 protein in regulating and controlling the drought resistance of plants. The maize drought resistance related gene resources are enriched, the maize drought resistance gene can be used for improving maize, the drought resistance of maize is improved, and the maize drought resistance gene has the advantages of being rapid in breeding and accurate in orientation. The invention has great application and popularization value for drought-resistant plant breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for cultivating plants with altered drought resistance, and a ZmMADS27 protein and its encoding gene. Background technique [0002] Drought and water shortage have become constraints to the world's agricultural and social development. In my country, irrigation water accounts for about 80% of the domestic water resources consumption, the actual utilization rate of natural precipitation in agriculture is less than 10%, and the effective utilization rate of agricultural irrigation water is only 30%-40%. my country's corn planting area is 24 million hectares, more than 50% of which are planted on dry land in the northeast, northwest and southwest regions that rely on natural precipitation and lack of water. The annual precipitation in these areas ranges from 200 to 600 mm. In some places, the evaporation is large, the water loss is fast, and the precipitation is very variable. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H5/10A01H6/46
CPCC07K14/415C12N15/8273
Inventor 王瑜巩志忠王亚琳
Owner CHINA AGRI UNIV
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