Group of laminaria japonica base morphology QTLs and breeding application thereof

A technology of kelp and base, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of less research on kelp QTL genetic analysis, fewer primer sequences, and molecular breeding, etc., to achieve enrichment Effect of Screening Breeding Resources

Inactive Publication Date: 2019-04-02
OCEAN UNIV OF CHINA
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, compared with terrestrial crops, kelp QTL genetic analysis research is less
Three QTLs for leaf length traits and two QTLs for leaf width traits have been reported (Liu FL, et al. QTL mapping for frond length and width Laminaria japonica Aresch Using AFLP and SSR Markers. Mar Biotechnol 2010,12:386-394 .); In addition, there are few primer sequences published about the QTL molecular markers of Laminaria, which is far from meeting the needs of molecular breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Group of laminaria japonica base morphology QTLs and breeding application thereof
  • Group of laminaria japonica base morphology QTLs and breeding application thereof
  • Group of laminaria japonica base morphology QTLs and breeding application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Construction of genetic population

[0053] Cross the female gametophytes of S. longissima (S. longissima) with the male gametophytes of S. japonica. After the F1 generation of kelp is bred at sea, select individuals with excellent traits and both parental traits for indoor collection of spores and self-breeding f 2 generation.

Embodiment 2

[0054] Embodiment 2: the construction of genetic map

[0055] (1) Using the CTAB method to extract the parents and 102 F 2 Separate the genomic DNA of the population, and dilute the qualified DNA to 50ng / μL, and use 1% agarose gel electrophoresis to detect the purity and integrity of the DNA;

[0056] (2) Using 49 pairs of AFLP, 88 pairs of SRAP and 1546 pairs of SSR primers developed in the laboratory, the parents and randomly selected F 2The two individuals of the isolated group were used as templates for PCR amplification, and the products were electrophoresed in denatured polyacrylamide gels. After staining and developing, the size of the bands was judged, and 37 pairs of polymorphic AFLP primers, 22 pairs of polymorphic AFLP primers, and 22 pairs of polymorphic primers were screened out. Morphic SRAP primers and 139 pairs of polymorphic SSR primers;

[0057] (3) Using polymorphic primers for 102 F 2 The isolated population was analyzed for molecular markers to obtain g...

Embodiment 3

[0062] Embodiment 3: QTL analysis of kelp base morphology

[0063] combined parent and F 2 The phenotypic data was analyzed by using the Composite Interval Mapping (CIM) of the software MapMaker for QTL analysis, and the results are shown in Table 2.

[0064] Table 2 Analysis results of QTLs for basal morphology of Laminaria

[0065]

[0066]

[0067] The inventor obtains a group of basal morphological QTLs of Laminaria qtl-jbxt-02 and qtl-jbxt-28 (such as figure 2 shown).

[0068] The above qtl-jbxt-02 is located in the linkage group LG02; the molecular markers adjacent to the above qtl-jbxt-02 are A20f380-S244f303, which are located by molecular markers A20f380 and S244f303.

[0069] The above qtl-jbxt-28 is located in the linkage group LG28; the molecular markers adjacent to the above qtl-jbxt-28 are H1057f16-A18f410, which are located by molecular markers H1057f16 and A18f410.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a group of laminaria japonica base morphology QTLs, molecular markers thereof and a breeding application thereof, relates to the field of molecular breeding. The QTLs related to morphology of laminaria japonica bases are explored and used in the breeding of laminaria japonica base morphological traits. The provided QTL related to the morphology of the laminaria japonica bases is qtl-jbxt-02 which is located by molecular markers of A20f380 and S244f303. Another provided QTL related to the morphology of the laminaria japonica bases is qtl-jbxt-28 which is located by molecular markers of H1057f16 and A18f410. The molecular markers and QTLs assist in screening the morphology of the laminaria japonica bases, characteristic amplification bands of the molecular markers are only need to be detected to predict the morphology of the laminaria japonica bases, an identification method is simple and selection efficiency is high.

Description

technical field [0001] The invention relates to the field of molecular breeding, in particular to a group of kelp basal morphological QTLs, molecular markers and breeding applications thereof. Background technique [0002] Kelp is an important large brown algae, rich in iodine, fucoidan, mannitol, alginate and other substances. It can be widely used in food, medicine, chemical industry, ecology, agriculture and other fields, with rich economic value. The main cultured species of kelp in my country are kelp, long-leaved kelp (S.longissima), Rishiri kelp (S.ochotensis) and some hybrid varieties. With the increase of people's demand for kelp products, improved varieties play a vital role in the development of kelp industry. Chinese kelp researchers have successively improved kelp varieties through continuous self-directed breeding, hybrid breeding, haploid breeding, etc., and cultivated "Haiqing series", "901", "Dongfang 2", "Dongfang 3 "And other relatively good kelp varieti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘涛
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap