A Rapid Breeding Method Based on Ion Implantation for Artificial Hybrids of Eucalyptus valeriana

A technology of ion implantation and hybridization, applied in planting substrates, seed and rhizome treatment, botany equipment and methods, etc., can solve the problems of luck and other factors

Active Publication Date: 2018-05-11
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of ion implantation is that there is an element of luck in the same way that a good new variety can be selected in one step in the natural forest.

Method used

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  • A Rapid Breeding Method Based on Ion Implantation for Artificial Hybrids of Eucalyptus valeriana
  • A Rapid Breeding Method Based on Ion Implantation for Artificial Hybrids of Eucalyptus valeriana
  • A Rapid Breeding Method Based on Ion Implantation for Artificial Hybrids of Eucalyptus valeriana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Embodiment 1 The selection of female parent and male parent seed

[0079] (1) Select female parent and male parent pollen to determine provenance

[0080] The female Eucalyptus urophylla was identified as the 14534 excellent provenance of Mt. Egon in Indonesia, and the pollen of the male parent Eucalyptus urophylla came from the 13659 excellent provenance of North of Laura, Queensland, Australia; these two provenances were confirmed to be their own tree species by early determination excellent provenance.

[0081] (2) Collection and preparation of seeds, and treatment of controls

[0082] From September to December every year, it is the flowering season of Eucalyptus urophylla. The pollen of Eucalyptus urophylla excellent tree collected in advance is pollinated on the stigma of the flower buds of Eucalyptus urophylla without stamens in bagging, and hybrid seeds of Eucalyptus urophylla are obtained. Collect capsules and artificial hybrid seeds in April, and collect the...

Embodiment 2

[0091] Embodiment 2 The making of seed carrying plate and seed placement treatment

[0092] (1) Production of aluminum carrier plate

[0093] Select an aluminum plate with a thickness of 2.25 mm and cut the plate size to 6.5×6.5 cm 2 ; The surface or surroundings of the aluminum plate are smooth and flat, and the center of the plate increases to 6×6 cm 2 seed spreading and processing space, that is, the actual injection area is 6×6 cm 2 , the one-time seed injection volume can be increased and the injection plate is more uniformly irradiated, thus saving the total injection time.

[0094] (2) Handling of the carrier plate

[0095] Stick double-sided tape on the injection area to adhere or isolate seeds of different strains; prepare multiple holding plates for holding seeds of different strains when injecting, and inject multiple strains at the same time when injecting at the same concentration; see figure 1 .

[0096] (3) Seed placement

[0097] Put 500-1,000 pure seeds ...

Embodiment 3

[0098] Example 3 A N-based + Rapid Breeding Method of Ion Implantation Artificial Hybrid of Eucalyptus valeriana

[0099] The experiment was carried out with the hybrid species of Eucalyptus spp., early injection: September in Zhengzhou; sowing seedlings: Guangzhou, from October to April of the following year:

[0100] (1) Injection method

[0101] 2.25 mm thick, 6×6 cm 2 The implanted seeds, such as artificial hybridization of eucalyptus with tails on the carrier plate, are placed in a vacuum N ion implanter. The ion implantation conditions are: a domestic high-energy ion implanter with a magnetic field strength of 3.0×10 -2 T, the current intensity is 2A, the injection beam range is 2.5 (μA), 50~80 KeV, 3×10 15 e / cm 2 Dose, inject the vacuum degree of the target chamber to 10 -3 Pa, in N + Ion implantation, at this time, the implantation time needs to be 1-2 h;

[0102] If using 1×10 15 e / cm 2 dose, the injection time is short and the treatment effect is not goo...

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Abstract

The invention discloses a rapid breeding method of Eucapyptus urophylla + E. tereticornis artificial hydrids based on ion implantation. According to the method, Eucapyptus urophylla + E. tereticornis artificial hydrids which are obtained by using Eucalyptus urophylla as the female parent and Eucalyptus tereticornis as the male parent are used as the material for breeding and implantation; by improving thickness and size of seed trays and optimizing ion implantation conditions, plant variation is rapidly realized; and finally by optimizing seedling and management technology and selecting proper testing index and standard, rapid selection of aim characteristics and continuous breeding material is realized. Within short time, excellent variant strains of Eucapyptus urophylla + E. tereticornis artificial hydrids are obtained. According to the invention, positive variation frequency of Eucapyptus urophylla + E. tereticornis artificial hydrids is raised, positive variant rate of seedling stage can reach 28.3%, damage is small, variant character is stable, the current generation can be utilized, and breeding cycle is greatly shortened.

Description

technical field [0001] The invention belongs to the fields of forestry biotechnology breeding and tree cultivation, and in particular relates to a rapid breeding method based on ion implantation of artificial hybrids of Eucalyptus valeriana. Background technique [0002] Eucalyptus belongs to Myrtaceae, native to Australia and its neighboring islands, and has been introduced to tropical and subtropical regions around the world in the past hundred years. Eucalyptus can be used not only for afforestation, but also as an important industrial timber species and an excellent raw material for pulp and paper making, which has good social, ecological and economic benefits. Eucalyptus is suitable for growing in sunny, warm and humid, fertile and loose sites. It can tolerate drought and barrenness. It is widely planted in low mountains and hills in South China, and is suitable for various site types such as iron brick red soil, red red soil, and mountain red red soil. Eucalyptus has ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01C1/00A01G9/029A01G17/00
CPCA01C1/00A01G22/00A01G24/00A01H1/02Y02A40/22Y02P60/40
Inventor 李光友徐建民陆钊华吴世军
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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