Long genome segment deletion system and use thereof

Abstract

The invention discloses a long genome segment deletion system. The long genome segment deletion system comprises two transcription activator-type effector TALENs of two target sequences of a specific splicing gene and is used for long genome segment deletion of a target gene after gene combination. The invention also discloses a use of the long genome segment deletion system. The long genome segment deletion system is convenient for use, is used for breeding of species producing large hereditary variation, provides rich genetic resources for breeding and has wide application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a genome long fragment deletion system, and also relates to the application of the genome long fragment deletion system. Background technique [0002] Bombyx mori is an important economic insect and a model organism of Lepidoptera. It has been bred and domesticated for more than 5,000 years in my country. The silk industry has always been in harmony with agriculture in history, and has made great contributions to the development of the Chinese nation's economy and the promotion of culture. Silk has inherent excellent characteristics such as graceful and elegant luster, light and comfortable touch, good drapability and curve of clothing materials, moderate water absorption and affinity to human skin, etc., and has been loved by consumers for a long time. Silk is also known as the "fiber queen" for this reason. At present, the total annual output value of my country's silk industry h...

AI Technical Summary

Problems solved by technology

Major breakthroughs have not been made in the problems of silkworm varieties such as robustness, silk quality, yield and other main economic traits, and the inherent defects of silk clothing such as easy yellowing and wrinkling have not been fundamentally resolved.

The update of variety resources is far behind the production needs, and the overall production efficiency of the silk industry is low

This is mainly because: 1) After hundreds of years of excavation, the potential of increasing production based on traditional breeding methods has been maximized. In addition to the particularity and limitations of natural conditions, it is difficult to break through the limitations of the variety resources themselves.

Although there are many types and quantities of silkworm species in existence, their genetic background is single, and there is no significant difference in their economic and biological traits.

After a hundred years of change of silkworm breeds, there is no significant improvement in economic and biological traits

2) As a textile fiber, silk is so expensive that many consumers are discouraged. In addition, silk is prone to yellowing and wrinkling, so it does not occupy a large market share in various textile fibers.

3) For a long time, the main purpose of human domestication and breeding of silkworms is to reel and weave silk, so the existing silkworm varieties are almost all the same multi-filament varieties, and the composition and characteristics of their cocoons are basically the same

Although the currently widely used molecular improvement tools such as transgenic and RNAi have been well applied in silkworms, there have been no reports of technologies that can directly edit and transform endogenous genes in silkworms, let alone deletion of long-segment genome sequences. Related reports

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