Ionic liquid loaded microencapsulated penicillium purpureum cell and preparation method and application thereof

A technology for producing Penicillium purpuricum and ionic liquid, which is applied in biochemical equipment and methods, microorganism-based methods, immobilized on or in inorganic carriers, etc. The effect of easy recovery and high biocatalytic performance

Pending Publication Date: 2019-07-12
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a preparation method of microencapsulated Penicillium purpura-producing cells loaded with ionic liquids, to solve the problem that the biosynthesis of GAMG by existing microencapsulated cells is relatively low compared to isolated cells

Method used

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  • Ionic liquid loaded microencapsulated penicillium purpureum cell and preparation method and application thereof
  • Ionic liquid loaded microencapsulated penicillium purpureum cell and preparation method and application thereof
  • Ionic liquid loaded microencapsulated penicillium purpureum cell and preparation method and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0042] Prepare a solution of CMC (sodium carboxymethylcellulose) at a concentration of 1.4% w / v and a solution of CaCl at a concentration of 1.0% w / v 2 (Calcium chloride) mixed solution, sterilized by high-pressure steam at 121°C for 20 minutes, after the solution was naturally cooled to room temperature, 15% v / v of [MBPy]Tf irradiated by ultraviolet light was added under sterile conditions 2 N, magnetically stir the mixed solution until the ionic liquid is evenly dispersed in the solution, then add 3% v / v bacterial suspension (the strain is Penicillium purpurogenum CGMCC No.446), and magnetically stir evenly. The mixed solution was dripped into the 1.2% w / v SA (sodium alginate) solution by magnetic stirring (120r / min) at a constant speed through the peristaltic pump (120r / min), spherical microencapsulated cells appeared rapidly in the SA solution, and the dripping Stir for 5 minutes after the addition, then filter the SA solution with a sieve with a pore size of about 1mm, ri...

Embodiment 12

[0045] Prepare a solution of CMC (sodium carboxymethylcellulose) at a concentration of 1.0% w / v and a solution of CaCl at a concentration of 0.5% w / v 2 (Calcium chloride) mixed solution, sterilized by high-pressure steam at 121°C for 20min, after the solution was naturally cooled to room temperature, 5% v / v of [MBPy]Tf irradiated by ultraviolet light was added under sterile conditions 2 N, magnetically stir the mixed solution until the ionic liquid is evenly dispersed in the solution, then add 2% v / v bacterial suspension (the bacterial strain is Penicillium purpurogenum CGMCC No.446, and magnetically stir evenly. The mixed solution is passed through Peristaltic pump (120r / min) drips into 0.6% w / v SA (sodium alginate) solution with magnetic stirring (120r / min) at a constant speed, spherical microencapsulated cells appear rapidly in the SA solution, stir after the addition 5min, then filter the SA solution with a sieve with a pore size of about 1 mm, rinse the formed microencaps...

Embodiment 13

[0047] Prepare a solution of CMC (sodium carboxymethylcellulose) at a concentration of 1.8% w / v and a solution of CaCl at a concentration of 0.25% w / v 2 (Calcium chloride) mixed solution, sterilized by high-pressure steam at 121°C for 20min, after the solution was naturally cooled to room temperature, 30% v / v of [MBPy]Tf irradiated by ultraviolet light was added under sterile conditions 2 N, magnetically stir the mixed solution until the ionic liquid is uniformly dispersed in the solution, then add 6% v / v bacterial suspension (bacterial strain is Penicillium purpurogenum (Penicillium purpurogenum) CGMCC No.446, and magnetically stir evenly. This mixed solution is passed through Peristaltic pump (120r / min) drips into 1.4% w / v SA (sodium alginate) solution with magnetic stirring (120r / min) at a constant speed, spherical microencapsulated cells appear rapidly in the SA solution, stir after the addition 5min, then filter the SA solution with a sieve with a pore size of about 1 mm,...

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Abstract

The invention discloses an ionic liquid loaded microencapsulated penicillium purpurogenum cell and a preparation method and application thereof. The preparation method comprises the following steps of: providing a mixed solution containing sodium carboxymethyl cellulose, calcium chloride and [MBPy] Tf2N, adding a bacterial suspension of penicillium purpurogenum CGMCC No. 446, then adding the uniformly mixed solution into a sodium alginate solution dropwise, shaping to obtain a microencapsulated cell, and immersing the microencapsulated cell into a calcium chloride solution for solidification to obtain the loaded ionic liquid microencapsulated penicillium purpurogenum cell. The invention further discloses the application of the ionic liquid loaded microencapsulated penicillium purpureum cell in biosynthesis of glycyrrhetinic acid monoglucuronide. According to the ionic liquid loaded microencapsulated penicillium purpurogenum cell and the preparation method and application thereof, the GAMG yield change of the ionic liquid loaded microencapsulated penicillium purpureum cell based on the culture time is close to that of the culture process of free cells, so that the microencapsulatedsystem has excellent biocatalytic efficiency.

Description

technical field [0001] The invention relates to a microencapsulated cell, in particular to a microencapsulated cell loaded with an ionic liquid and a preparation method and application thereof. Background technique [0002] Microencapsulated cell technology provides technical support for increasing cell culture density, enhancing adversity tolerance, increasing the conversion of substrates, improving the stability and reuse of microbial whole-cell catalysis in the free cell culture process. However, the microencapsulation environment is different from the free cell culture process. Studies have found that cells in the microencapsulation environment have growth lag, metabolic lag, and reduced differentiation levels. [0003] Ionic liquids (ILs) are generally room temperature molten salts composed of anions and cations. Because of their unique physical and chemical properties, ILs have been widely used in the field of biology. When ionic liquid acts as a biocatalytic medium o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/12C12N1/14C12P33/00C12R1/80
CPCC12N1/14C12N11/12C12N11/14C12P33/00
Inventor 曹红欧阳巧凤谢睆
Owner JIAXING UNIV
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