Penicillium purpurogenum as well as biological preparation and application thereof in controlling litchi downy blight

A technology for producing Penicillium purpuricum and Phytophthora litchi, applied in the direction of chemicals for biological control, biocides, microorganism-based methods, etc., to achieve the requirements of low culture conditions, strong inhibitory effect, and excellent biocontrol effect. Effect

Active Publication Date: 2014-05-07
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective measures for the control of litchi downy mildew disease are mainly based on chemical control, but due to the environmental pollution caused by the extensive use of chemical pesticides and the problem of drug resistance of pathogens are becoming more and more promin

Method used

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  • Penicillium purpurogenum as well as biological preparation and application thereof in controlling litchi downy blight
  • Penicillium purpurogenum as well as biological preparation and application thereof in controlling litchi downy blight
  • Penicillium purpurogenum as well as biological preparation and application thereof in controlling litchi downy blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Isolation, purification and preservation of producing Penicillium purpura in litchi

[0027] (1) PDA medium configuration: Weigh 200g of potatoes, wash, peel and chop them, add 1000mL of water to boil for 0.5h, filter with gauze, add 20g of glucose and 20g of agar, fully dissolve, filter with hot gauze, and sterilize at 121°C After 20 minutes, divide into petri dishes and store for later use after cooling.

[0028] (2) Isolation and purification of Penicillium purpura producing in litchi:

[0029] The lychee fruit samples used for separation were collected from the lychee base of the College of Horticulture of South China Agricultural University, the lychee garden of Shenzhen Qilin Villa and the Shenzhen Baoan Nongke Orchard. The selected lychee varieties were Nuomici, Feizixiao and Guiwei. species, divided into three groups.

[0030] Refer to the conventional tissue separation method with slight modifications: wash the healthy tissue (leaves, fruits) wi...

Embodiment 2

[0036] Embodiment 2: The confrontation culture method measures the activity of Penicillium purpurea

[0037] (1) The preparation of PDA medium is the same as in Example 1; the preparation of PDB liquid medium is the same as that of PDA solid medium except that agar is not added. bottle (100mL culture medium), stoppered, bandaged, sterilized at 121°C for 20min, cooled and stored for later use.

[0038] (2) Preparation of carrot medium: Weigh 200g of carrot, squeeze the juice with a juicer, filter it with 16 layers of gauze, make up the filtrate to 1000mL of water, add 15g of agar, fully heat and dissolve, and then pack it into conical flasks ( 100mL culture medium per bottle), sterilized at 121°C for 20min, cooled and stored for later use.

[0039] (3) Preparation of single colony and plate of Penicillium purpura: Use an inoculation needle to pick out the mycelium of Penicillium on a PDA medium plate and culture at 28°C for 4-5 days to obtain a single colony; Holes are punche...

Embodiment 3

[0048] Embodiment 3: Production of Penicillium purpura GY-19 in litchi prevents litchi downy mildew

[0049] (1) The preparation of PDA medium is the same as in Example 1. The preparation of carrot medium and PDB liquid medium is the same as in Example 2.

[0050] (2) The preparation of the fermentation broth and filtrate of Penicillium purpura strain GY-19 produced in litchi, the cultivation of Lychee downy mildew and the preparation of its sporangia suspension are the same as in Example 2.

[0051] (3) Pick healthy lychee fruits with uniform maturity and uniform size, rinse them in running water, and dry them in the air. Put the lychee fruits in the fermentation liquid of Penicillium purpurea and its sterile filtrate prepared in step (2) respectively. Soak for about 5 minutes, take it out until the liquid drops stop dripping, and put it in a sterile fresh-keeping box for moisturizing treatment. The method of spraying inoculation of sporangia of Lychee peronospora (10 4 pe...

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Abstract

The invention belongs to the field of biological pesticides, and discloses a litchi endogenetic Penicillium purpurogenum which is prepared by taking litchi endogenetic microorganisms as screening objects through separating and has a significant inhibition effect on litchi downy blight, as well as a biological preparation and an application thereof in controlling litchi downy blight. The Penicillium purpurogenum, with a Preservation Number of CCTCC NO:M2013738, is named 'Penicillium purpurogenum' GY-19, preserved in China Center for Type Culture Collection (CCTCC) on December 31, 2013 at Wuhan University, Wuhan, China. The strain is from litchi endogenetic fungi, can be colonized on the surfaces of litchi fruits well, and has a strong inhibition effect on litchi downy blight, therefore, the Penicillium purpurogenum has an excellent biocontrol effect on controlling litchi downy blight; and the Penicillium purpurogenum is environment-friendly and non-toxic in biogenetic derivation and low in culture conditions, and has a small influence on the ecological environment, therefore, the Penicillium purpurogenum has an extremely good development prospect.

Description

technical field [0001] The invention belongs to the field of biopesticides, and in particular relates to a strain of Penicillium purpurea produced in litchi which has a significant inhibitory effect on litchi downy mildew isolated from litchi endophytic microorganisms as the screening object, and biological preparations thereof and the prevention and control of litchi downy mildew disease application. Background technique [0002] Litchi (Litchi chinensis Sonn) is an important subtropical economic crop in the southern region. It is known as "the precious fruit of China". It has high economic value and is one of the most competitive fruits in the international market in my country. However, in the main producing areas of litchi in my country, downy mildew caused by Peronophythora litchii Chen ex Ko et al. is one of the most important diseases that cause litchi yield reduction. The prevalence of the disease has the characteristics of indirect outbreaks and is greatly affected...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/80
Inventor 习平根江立群徐丹丹姜子德许再元陈金红李敏慧黄陆浩钟瑾
Owner SOUTH CHINA AGRI UNIV
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