Method for separation and purification of zeaxanthin from capsanthin

Abstract

Belonging to the technical field of carotenoid preparation, the invention discloses a method for separation and purification of zeaxanthin from capsanthin. The method is characterized by including thepreparation steps of: subjecting capsanthin, alcohol, alkali and an antioxidant to saponification reaction; adjusting the pH value of the saponification product to 7-8, performing diluting a n-hexane-acetone-ethyl acetate mixed solution, carrying out desorption, and collecting a zeaxanthin desorption solution; recovering the solvent, and conducting cooling and crystallizing; and carrying out filtering, washing and drying to obtain zeaxanthin crystals. The method provided by the invention directly adopts capsanthin as the raw material, performs saponification treatment to obtain a zeaxanthin monomer, and then separate zeaxanthin with a content of 90% or above. The product is convenient for large-scale production.

Description

technical field [0001] The invention discloses a method for separating and purifying zeaxanthin from capsanthin, which belongs to the technical field of carotenoid preparation. Background technique [0002] Capsanthin, or capsanthin, is a tetraterpenoid orange-red pigment present in ripe red pepper fruits, which belongs to carotenoid pigments. Among them, the more polar red components are capsanthin and capsanthin, accounting for 50%~60% of the total, and the other is the less polar yellow component, the main components are zeaxanthin and carrot white. [0003] Zeaxanthin (Zeaxanthin, 3,3'-dihydroxy-β-carotene), also known as zeaxanthin, has a molecular formula of C40H56O2 and a molecular weight of 566.88. It belongs to the isoprene class and is an oxygen-containing carotenoid. It is isomer with lutein. With good antioxidant properties, it has unique functions in relieving visual fatigue, preventing age-related macular degeneration, protecting skin, enhancing immunity and...

AI Technical Summary

Problems solved by technology

[0004] The applicant found in the research: first, there is an urgent need for a method that can efficiently extract natural zeaxanthin; with the continuous development of toxicology and analysis techniques, studies have shown that although the purity of chemically synthesized zeaxanthin is relatively high , the production cost is low, but the product is easily mixed with a small amount of toxic chemicals, which can cause chromosomal mutation and carcinogenesis, so with the continuous improvement of awareness, naturally extracted zeaxanthin will occupy an active position in the market

Second, the natural zeaxanthin obtained by the existing separation method has low purity and high price; in the field of extracts, the purity is directly proportional to the price. When the purity of a single component reaches more than 80%, as the purity of a single component of the extract increases Increasing its selling price will increase exponentially; in order to obtain high-purity natural zeaxanthin, the existing methods are mostly supercritical extraction and subcritical fluid extraction. This method is extremely expensive and low in output, and cannot be applied to large-scale industrial production However, the relatively low-cost analytical column adsorption separation method has unsatisfactory purity and low yield.

Third, due to the problem of impurities, in the prior art, it is necessary to separate and remove impurities multiple times to obtain high-purity zeaxanthin, and there must be a loss of zeaxanthin in each separation, which leads to an unsatisfactory yield and causes raw material loss. waste, increased cost