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Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof

A technology for producing Penicillium purpura and plants, applied in botany equipment and methods, microbial-based methods, biochemical equipment and methods, etc., can solve the high cost of chemical nematicides, teratogenicity, side effects of parasitic nematode natural enemies, etc. question

Active Publication Date: 2016-02-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in recent years, chemical nematicides are not only costly, but also have unsatisfactory control effects. At the same time, there are few new nematicides developed and the range of choices is narrow. The use of most varieties will kill some carnivorous nematodes and nematodes that prey on plant pathogenic nematodes. Soil-dwelling young animals have great side effects on the activation of soil, ecological protection, and the reproduction of natural enemies of parasitic nematodes, and some species even have the risk of teratogenicity, carcinogenicity, or affecting fertility (Hou Jinli. 2015. Research on the Prevention and Control of Plant Parasitic Nematodes in my country Progress. Modern Agricultural Science and Technology. 7:136)

Method used

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  • Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof
  • Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof
  • Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0042] Example 1. Isolation and Identification of Penicillium purpurogenum MHZ111

[0043] 1.1 Strain isolation

[0044] Strain MHZ111 was isolated from permafrost in Mohe, Heilongjiang, China by dilution plate isolation method. Weigh 10g of Mohe permafrost and put it into 90ml sterile water, use a 1ml sterile pipette to draw 1ml10 -1 Concentration of bacterial solution in a tube of 9ml sterile water, shake well that is 10 -2 Concentration of bacterial solution, the same method, sequentially diluted to 10 -4 . the above 10 -2 、10 -3 and 10 -4 Spread the soil bacteria solution on the PDA plate respectively, dry it and place it upside down in an incubator at 25°C for 3-5 days, transfer the isolated fungus to the PDA slope for cultivation, and store it in the refrigerator after it grows well . The strain numbered MHZ111 was taken for the following identification.

[0045] 1.2 Strain identification

[0046] 1.2.1 Observation of strain morphology

[0047] Pick the strain...

Embodiment 2

[0062] Embodiment 2, plant parasitic nematode inhibitor and nematicidal activity thereof

[0063] 1. Cultivation of Penicillium purpurogenum MHZ111

[0064] 1.1 Test tube culture

[0065] Penicillium purpurogenum MHZ111 was inoculated into PDA medium and cultured at 25°C for 7 days to obtain test tube species.

[0066] Among them, potato dextrose agar (PDA) medium: peeled potatoes, cut 200g into small pieces, boiled with water for 30min, filtered through 4 layers of gauze, added 20g of glucose, 17g of agar, distilled water to 1000mL, boiled and mixed, 121°C Sterilize for 20 minutes to obtain PDA medium.

[0067] 1.2 Liquid fermentation culture of Penicillium purpurogenum MHZ111

[0068] Inoculate the test tube seed of 1.1 into a 500mL Erlenmeyer flask containing 125mL PD liquid medium, at 25°C-28°C, the shaker rotates at 200r min -1 ~220r·min -1 1. Ferment for 72 hours to obtain Penicillium purpurogenum MHZ111 fermentation broth.

[0069] Among them, potato dextrose (PD)...

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Abstract

The invention discloses penicillium purpurogenum for poisoning plant parasitic nematodes and a preparing method and application thereof. The bacterial strain number of the penicillium purpurogenum for poisoning plant parasitic nematodes is MHZ111, and the register number of the penicillium purpurogenum for poisoning plant parasitic nematodes in the China General Microbiological Culture Collection Center is CGMCC No.11630. A plant parasitic nematode inhibitor capable of being dissolved in ethyl acetate is a substance extracted from a penicillium purpurogenum MHZ111 culture by means of ethyl acetate. The root-knot nematode second-stage juvenile killing ability is obviously higher than that of abamectin and fosthiazate when the contents of active ingredients are 1.5 g / L and 1.0 g / L respectively. Incubation of root-knot nematodes can hardly be achieved when the contents of the active ingredients are both 1.5 g / L. The root-knot nematode oocyst incubation restraining effect is similar to that of abamectin and fosthiazate when the contents of active ingredients are 1.0 g / L and 1.5 g / L respectively.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a Penicillium purpuragenes poisoning and killing plant parasitic nematodes and a preparation method and application thereof. Background technique [0002] Plant parasitic nematodes refer to a type of nematodes that can parasitize various tissues of plants, make plants stunted, and transmit other plant pathogens while infecting hosts, causing plant disease symptoms. It occurs and harms many countries and regions in the world, and there are various types. At present, there are more than 200 genera and 5,000 species of plant parasitic nematodes recorded in the world (Chen Lijie, Duan Yuxi. 2006. Classification and identification of plant parasitic nematode species resources. Chinese Journal of Parasitology and Parasitic Diseases, 24:s29-33 ). It is estimated that the global annual losses caused by plant parasitic nematodes to agriculture and forestry produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P5/00C12R1/80
Inventor 董丹刘伟成刘霆张涛涛吴慧玲田兆丰刘德文张殿鹏卢彩鸽赵娟
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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