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Process for preparing ceramide by fermentation method

A technology of ceramide and fermentation method, applied in the field of preparation technology of ceramide, can solve the problems of ceramide safety doubt, ceramide safety hazard and high economic cost, and achieves the advantages of quick effect, short fermentation period and reduced economic cost. Effect

Pending Publication Date: 2016-11-16
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing ceramides are all obtained by separating animals and plants, and the yield is low and the economic cost is high.
With the occurrence of animal diseases such as mad cow disease, the safety of animal-derived ceramide has been questioned, and the safety of chemically synthesized ceramide in industry is also relatively harmful to the human body.

Method used

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  • Process for preparing ceramide by fermentation method
  • Process for preparing ceramide by fermentation method
  • Process for preparing ceramide by fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Analysis of strains, preparation and identification of ceramide

[0042] 1. Morphological observation and genomic DNA sequence analysis of Penicillium purpuragenicum EFOZ-02

[0043] (1) Morphological observation of Penicillium purpogenum EFOZ-02 strain

[0044] The morphology of the strain was observed as Figures 1 to 8 shown.

[0045] (2) Genomic DNA sequence analysis

[0046] Genomic DNA sequence analysis such as Figure 9 shown by Figure 9 It can be seen that the ITS gene region of Penicillium purpogenum in area 95 is the most similar to our strain EFOZ-02, and 95 is the closest to our strain.

[0047] 2. Preparation of ceramide

[0048] (1) Preparation of culture medium

[0049] A. Seed medium (Cha's medium)

[0050] Ingredients: 3g sodium nitrate, 1g dipotassium hydrogen phosphate, 0.5g magnesium sulfate, 0.5g potassium chloride, 0.01g ferrous sulfate, 30g sucrose, 1000mL distilled water.

[0051] Preparation method:

[0052] ① Weighing and ...

Embodiment 2

[0082] Example 2 Preparation of Ceramide

[0083] (1) Preparation of culture medium

[0084] A. Seed medium (Cha's medium)

[0085] Ingredients: sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1000mL.

[0086] Preparation method:

[0087] ①Weighing and melting: Measure about 2 / 3 of the required amount of water and add it to the beaker. Weigh sucrose, sodium nitrate, dipotassium hydrogen phosphate, potassium chloride, and magnesium sulfate, respectively, and add them into water one by one to dissolve. Add 1 mL of 0.1% ferrous sulfate solution to ml medium;

[0088] ②Add all the remaining water;

[0089] ③ Packing, plugging and dressing;

[0090] ④ High pressure steam sterilization: 100 Pa sterilization for 20 min.

[0091] B. Fermentation medium (PDA medium)

[0092] Ingredients: 200g potatoes, 20g sucrose, 2g peptone, 3g dipotassium hydrogen phosphate, 1g magnesiu...

Embodiment 3

[0110] Example 3 Preparation of Ceramide

[0111] (1) Preparation of culture medium

[0112] A. Seed medium (Cha's medium)

[0113] Ingredients: sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1000mL.

[0114]  Preparation method:

[0115] ①Weighing and melting: Measure about 2 / 3 of the required amount of water and add it to the beaker. Weigh sucrose, sodium nitrate, dipotassium hydrogen phosphate, potassium chloride, and magnesium sulfate, respectively, and add them into water one by one to dissolve. Add 1 mL of 0.1% ferrous sulfate solution to ml medium;

[0116] ②Add all the remaining water;

[0117] ③ Packing, plugging and dressing;

[0118] ④ High pressure steam sterilization: 100 Pa sterilization for 20 min.

[0119] B. Fermentation medium (PDA medium)

[0120] Ingredients: 200g potatoes, 20g sucrose, 2g peptone, 3g dipotassium hydrogen phosphate, 1g magnesi...

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Abstract

The invention provides a process for preparing ceramide by a fermentation method. The process comprises the technical flow processes of preparation of a culture medium, culture of penicillium purpurogenum EFOZ-02, sample preparation, HPLC (High Performance Liquid Chromatography) separation, drying and the like. When the process provided by the invention is used for producing the ceramide, the penicillium purpurogenum EFOZ-02 can secrete a great number of ceramide type substances into fermentation liquid, so that an any derivatization process is not needed; the operation is simple; the extraction is convenient; the microbial fermentation period is short; the effect taking speed is high; the limitation by climates in districts is avoided; importantly, in reported studies about the penicillium purpurogenum at prevent, the existence of the existing toxin in metabolites is not discovered; certain safety is ensured; by using the process provided by the invention for producing the ceramide, the economic cost can be greatly reduced; and the large-scale industrial production of the ceramide can be promoted. Therefore the process has huge market application values.

Description

technical field [0001] The invention relates to a process for preparing ceramide, more particularly to a process for preparing ceramide by fermentation. Background technique [0002] With the advancement of science and technology and the improvement of living standards, people expect to obtain biological preparation products with extensive practical applications and huge economic value. Ceramide undoubtedly meets various needs of people. [0003] Ceramide is an amide formed by the condensation of sphingosine and long-chain fatty acids, and is mainly present in the cell membrane and intercellular matrix of the stratum corneum. Ceramide was first isolated from the nerve sheath of animals, hence the name. In the daily chemical beauty industry, ceramide can strengthen the anti-aging function, keep the skin elastic, smooth and delicate, and reduce the formation of facial wrinkles. In medicine, ceramide plays an important messenger role in inducing biological effects of various...

Claims

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Application Information

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IPC IPC(8): C12P13/02C12R1/80
CPCC12P13/02
Inventor 涂俊铭汪乐苏平
Owner HUBEI NORMAL UNIV
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