A method for preparing ceramide from rice bran by fermentation method

A technology of ceramide and fermentation method, which is applied in the field of fermentation engineering, can solve the problems of long production cycle, large energy consumption, large amount of organic solvent, etc., and achieve the effects of reducing production cost and environmental pollution, short fermentation cycle and simple operation

Active Publication Date: 2021-06-29
BEIJING POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of ceramide in rice bran is relatively high, as in the patents "composition containing ceramide extracted from rice bran as an active ingredient and having anti-atopic activity [(publication (announcement) number: CN105311041A]" and "a kind of The method for extracting high-purity ceramide from rice bran [(public (announcement) number: CN106008253B] ", all provide the use of organic solvents such as ethanol, petroleum ether, through long-term heating and leaching to prepare ceramide from rice bran However, due to the cell wall structure of plant cells, the substances in the wall are not easy to be separated and extracted, resulting in large energy consumption, large amount of organic solvent and low extraction yield during the extraction process.
However, in "A Process for Preparation of Ceramide by Fermentation [(Publication (Announcement) No.: CN106119310A]", Penicillium purpurea was used to prepare ceramide by fermentation. Although this invention simplifies the preparation process of ceramide, but Using methanol as a solvent in the preparation process, the residual methanol will cause harm to the human body, and the production cycle of the invention is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing ceramide from rice bran by fermentation, the method specifically comprising:

[0038] (1) Raw material processing: after drying the rice bran in an oven at 80°C, remove impurities, then crush and pass through a 20-40 mesh sieve;

[0039] (2) Strain activation: cut G. lucidum 1 cm from the slope with an inoculation hook 2 The slanted hyphae with the culture medium were inserted into the middle of the solid medium, and cultured at 30°C for 5 days;

[0040] (3) Seed liquid preparation: mash the solid medium with Ganoderma lucidum with an inoculation hook, then add appropriate amount of sterile water to obtain Ganoderma lucidum seed liquid;

[0041](4) Fermentation culture: mix Ganoderma lucidum seed liquid and rice bran raw material powder at a mass ratio of 5:1, put them into a fermenter, and cultivate them at 30°C for 5 days, and control the oxygen concentration in the space of the fermenter to 0.025mol / L ;

[0042] (5) Homogenizing and breaking...

Embodiment 2

[0046] A method for preparing ceramide from rice bran by fermentation, the method specifically comprising:

[0047] (1) Raw material processing: after drying the rice bran in an oven at 80°C, remove impurities, then crush and pass through a 20-40 mesh sieve;

[0048] (2) Strain activation: use an inoculation hook to cut G. leucocontextum 1 cm from the inclined surface 2 The slanted hyphae with the culture medium were inserted into the middle of the solid medium, and cultured at 28°C for 5 days;

[0049] (3) Seed liquid preparation: mash the solid medium with Ganoderma lucidum with an inoculation hook, then add appropriate amount of sterile water to obtain Ganoderma lucidum seed liquid;

[0050] (4) Fermentation culture: mix Ganoderma lucidum seed liquid and rice bran raw material powder in a mass ratio of 3:1, put them in a fermenter, and cultivate them at 28°C for 5 days, and control the oxygen concentration in the space of the fermenter to 0.02mol / L ;

[0051] (5) Homogen...

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Abstract

The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for preparing ceramide from rice bran by a fermentation method. The method comprises the following steps: after removing impurities, pulverizing and sieving the rice bran, adding Ganoderma lucidum to the rice bran powder for fermentation; separating the fermented liquid, taking the clear liquid to obtain a ceramide solution, and concentrating and crystallizing the obtained ceramide solution to obtain Ceramide solid. The present invention utilizes Ganoderma lucidum to ferment rice bran, which not only can significantly improve the dissolution of ceramide in rice bran, but also produces a certain amount of ceramide from Ganoderma lucidum. Environmental protection, and not subject to regional climate restrictions, can significantly reduce economic costs.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for preparing ceramide from rice bran by a fermentation method. Background technique [0002] Ceramide is N-fatty acyl sphingosine, also known as molecular nails and sialome, is a signal transduction substance widely present in eukaryotic cells, mainly as a structural component of cell membrane or intracellular Exists in free molecular form. Ceramide is an active ingredient in pharmaceuticals, food and cosmetics that has received much attention in recent years. According to reports, the biological functions of ceramide mainly include the following aspects: ① cell adhesion; ② regulation of cell proliferation and differentiation; ③ induction of apoptosis ; ③ regulation of protein secretion; ④ the role of inflammation. The application in cosmetics can act as a barrier to prevent the loss of water, and has the functions of protecting and repair...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C231/24C07C233/18C12P13/02C12R1/645
CPCC07C231/24C12P13/02C07C233/18
Inventor 闫征辛秀兰李双石于海龙刘卉
Owner BEIJING POLYTECHNIC
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