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Preparation method for seawater microbial flocculants

A microbial flocculant, flocculant technology, applied in the field of preparation of seawater microbial flocculant by using Penicillium purpura to achieve ideal flocculation effect

Inactive Publication Date: 2011-03-30
TIANJIN SEA WATER DESALINATION & COMPLEX UTILIZATION INST STATE OCEANOGRAPHI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no microbial flocculant specially used for seawater treatment. Therefore, in order to better serve seawater desalination and direct utilization, it is necessary to study microbial flocculants suitable for seawater flocculation treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, preparation fermentation medium

[0017] Take fresh potatoes and scrape off the skin. Weigh 200g of peeled potatoes and cut them into small pieces with a diameter of about 0.5cm. Add 1L of distilled water to a beaker, put in the cut potato pieces, and heat to boil. After boiling for 1 hour, stop heating, wait for the liquid to cool down, use 4 layers of gauze to filter and remove potato pieces, and obtain potato juice. Add 20 g of sucrose to the potato juice, add appropriate amount of distilled water to make up 1 L, and sterilize at 115° C. for 20 minutes to prepare 1 L of fermentation medium.

Embodiment 2

[0018] Embodiment 2, the fermentation culture of bacterial classification

[0019] Inoculate the Penicillium purpurea EL-O2 strain into the fermentation medium prepared in Example 1, and use the HDL HZQ-QX full-temperature shaking shaker to cultivate for 3 days at a temperature of 30°C and a rotation speed of 180rpm to obtain a purple-green strain Fermentation medium of mold species.

Embodiment 3

[0020] Embodiment 3, preparation seawater microbial flocculant finished product

[0021] The fermentation broth of Penicillium purpuragenes prepared in Example 2 was centrifuged using a Sigma 3K15 desktop high-speed refrigerated centrifuge, and centrifuged at a temperature of 4°C and a speed of 12000 rpm for 5 minutes. After centrifugation, pour 1L of the fermentation supernatant in the centrifuge tube into a beaker and place the beaker in an ice bath, add 2L of pre-cooled absolute ethanol to the liquid in the beaker, stir slightly at first, and then mix Stand still for 6h. Then it was centrifuged again by a high-speed refrigerated centrifuge, the centrifugation temperature was 4°C, the rotation speed was 12000rpm, and the centrifugation time was 5min. After centrifugation, collect the precipitate in the centrifuge tube, and use Boyikang FD-1A-50 freeze-vacuum dryer to freeze-vacuum-dry for 2 days at a temperature of -45°C and a vacuum of 13pa to obtain 1.128g of powdered sea...

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Abstract

The invention discloses a preparation method for seawater microbial flocculants. In the invention, penicillium purpurogenum separated and sieved from coast intertidal zone sludge is adopted to prepare the seawater microbial flocculants; potato juice is taken as raw materials to prepare fermentation medium; penicillium purpurogenum EL-O2 strains are inoculated in the fermentation medium, and are cultured at 25-35DEG C at shaker rotating speed of 160-200rpm to obtain ferment culture solution; the ferment culture solution is centrifugalized at a high speed under the conditions that the temperature is 4-8DGE C and the rotating speed is 8000-12000rpm to obtain ferment liquid supernatant; the ferment liquid supernatant is precipitated with ethanol and is centrifugalized at a high speed at 4-8DEG C and at the rotating speed of 10000-12000rpm to obtain precipitate; and the precipitate is subject to vacuum freeze-drying under the conditions that the temperature is 45DEG C below zero to 20DEG C below zero and the vacuum degree is lower than 15pa to obtain the finished seawater microbial flocculants. The seawater microbial flocculants having high flocculation activities and being specially used for sea water processing have ideal flocculation effect and simple process, and are convenient to apply and prompt.

Description

technical field [0001] The invention relates to a preparation method of a microbial flocculant, in particular to a method for preparing a seawater microbial flocculant by using Penicillium purpurea. Background technique [0002] With economic development and population increase, human demand for water resources is increasing year by year. In recent years, in order to alleviate the increasing shortage of fresh water resources, the development of seawater resources has become an important way to alleviate the tension of water resources, and many coastal cities have begun to implement seawater desalination and direct utilization technologies. Most of the water source for desalination and direct utilization is near-shore seawater, where the seawater is easily polluted and often contains a large amount of suspended solids, colloids, and microorganisms. Therefore, the primary problem to be solved for desalination and direct utilization of seawater is seawater purification. In the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/52C12P1/02C12R1/80C02F103/08
Inventor 张雨山王静张秀芝郝建安杨波郭鸿博
Owner TIANJIN SEA WATER DESALINATION & COMPLEX UTILIZATION INST STATE OCEANOGRAPHI
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